Expression of JhI-1^hs.T:SV5\V5 by heat pulses delivered daily allows the rescue JhI-1^ED24 mutants to adulthood. The adult flies do not require any additional heat pulses for their survival. Both male and female escapers not expressing JhI-1^hs.T:SV5\V5 any longer show low fertility and in a few days after eclosion they become completely sterile. Resuming JhI-1^hs.T:SV5\V5 expression restores their fertility. After 8 days without JhI-1^hs.T:SV5\V5 expression, oogenesis in JhI-1^ED24 females stalls at stages 8-9. Extra-numeral germ-line cells are observed in mutant egg chambers. In contrast to wild-type egg chambers that display a single oocyte connected to 15 nurse cells surrounded by a layer of follicle cells, mutant egg chambers can have three oocytes and more than 40 nurse cells of different size. Some mutant egg chambers have the follicle cell layer completely missing or composed of significantly fewer cells. Several mutant cysts fuse as they bud off from the germarium. Upon dissection of mutant males, mutant testes is found thinner than those of control males. The middle section of mutant testes is reduced in diameter compared with controls. Mutant testes display several defects. Compared with wild-type, mutant testes show a reduction in the number of mitotically dividing gonialblasts at the apical tip. All post-meiotic germ cells (e.g. onion stage or elongated spermatids) are absent or underrepresented in the mutants. Compared with wild-type, mutant testes contain about half the number of cysts of individualising spermatids that appear as bundles of needle-shaped nucei. The most abundant cysts of mutant testes are those with sixteen fully-grown primary spermatocytes. These pre-meiotic cells contain a large nucleus with partially condensed chromosomes and misshapen Nebenkerns indicating a failure of meiosis.

One heat-shock treatment is sufficient to rescue early lethality and developmental arrest in JhI-1^ED24 mutants expressing JhI-1^hs.T:SV5\V5. The mutants undergo two larval molts and reach third instar but still fail to attain wild-type size and never pupariate. Instead, the keep crawling in the food for about ten days and then die. Wing discs dissected from the rescued larvae are smaller, but do not show elevated levels of cell death.

The growth arrest caused by homozygous JhI-1^ED24 can be partially rescued with one or two heat pulses delivered to larvae carrying JhI-1^hs.T:SV5\V5. However, only endoreplicating tissues increase in size during this rescue period, mitotic tissues fail to reach wild-type size.