FB2024_03 , released June 25, 2024
Allele: Dmel\sasUAS.cLa
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General Information
Symbol
Dmel\sasUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lee
FlyBase ID
FBal0290173
Feature type
allele
Associated gene
Dmel\sas
Associated Insertion(s)
Carried in Construct
P{UAS-sas.L}
Key Links
Transgenic product class
cDNA
(Lee et al., 2013)
wild_type
(Lee et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lee et al., 2013)
wild_type
(Lee et al., 2013)
Mutagen
in vitro construct
(Lee et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-sas.L}
Cytology
Description

UASt regulatory sequences drive expression of a full-length (1693aa) sas cDNA.

(Lee et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
sas
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stage 16 embryos expressing sasScer\UAS.cLa under the control of Scer\GAL4repo.PU have a normal pattern of Fas2-positive axons, but ~5% of segments exhibit inner longitudinal bundle midline crossing. The glial lattice is well-organised.

      Stage 16 embryos expressing sasScer\UAS.cLa under the control of Scer\GAL4elav.PU have a normal pattern of Fas2-positive axons, but in ~14% of segments exhibit inner longitudinal bundle midline crossing.

      Stage 16 embryos expressing sasScer\UAS.cLa under the control of Scer\GAL4sim.PU have a normal pattern of Fas2-positive axons, but in ~5% of segments exhibit inner longitudinal bundle midline crossing.

      (Lee et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of sasScer\UAS.cLa under the control of Scer\GAL4Fas2-Mz507 almost completely rescues the CNS defects seen in sas15/Df(3R)ED5221, Ptp69D1/Df(3L)8ex25 double mutant embryos.

      Expression of sasScer\UAS.cLa under the control of Scer\GAL4Fas2-Mz507 almost completely rescues the CNS defects seen in sas15, Ptp69D1/Df(3L)8ex25 double mutant embryos.

      A Ptp10D1 mutant background enhances the ectopic midline crossing phenotype seen in stage 16 embryos expressing sasScer\UAS.cLa under the control of Scer\GAL4sim.PU. No effect is seen when sasScer\UAS.cLa is expressed under the control of Scer\GAL4elav.PU.

      A Ptp10D1 mutant background enhances the ectopic midline crossing phenotype seen in stage 16 embryos expressing sasScer\UAS.cLa in glia under the control of Scer\GAL4repo.PU. The inner Fas2-positive axon bundle crosses the midline, but the outer bundles usually do not. The pattern of glial nuclei is quite disorganised; the double row of nuclei along each longitudinal tract is replaced by aggregations of varying width, and there is no segmentally repeated pattern of nuclei near the midline.

      (Lee et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sasScer\UAS.cLa
      sasUAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Lee
      Secondary FlyBase IDs
        References (2)