A 3475 base pair fragment from the flanking non-coding or intronic region of norpA is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Ecol\lexAGMR15B03/diaRNAi.lexAop is a suppressor of neoplasia | larval stage phenotype of EgfrUAS.cBa, Scer\GAL4ap-md544, psqKK111691
Ecol\lexAGMR15B03/diaRNAi.lexAop is a suppressor of increased cell number | larval stage phenotype of EgfrUAS.cBa, Scer\GAL4ap-md544, psqKK111691
rprlexAop.cHa/Ecol\lexAGMR15B03 is a suppressor of neoplasia phenotype of EgfrUAS.cBa, Scer\GAL4ap-md544, psqJF03109
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of neoplasia | larval stage phenotype of Ras85DV12.UAS, Scer\GAL4ap-md544, dlg1GD4689
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of increased cell number | larval stage phenotype of Ras85DV12.UAS, Scer\GAL4ap-md544, dlg1GD4689
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of neoplasia | larval stage phenotype of BacA\p35UAS.cHa, Scer\GAL4hh.PU, rodGD8768
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of neoplasia | larval stage phenotype of Ras85DV12.UAS, Scer\GAL4hh.PU, dlg1GD4689
rprUAS.C, rprlexAop.cSa, Ecol\lexAGMR15B03, Scer\GAL4He.PZ is a non-suppressor of neoplasia | larval stage phenotype of Ras85DV12.UAS, Scer\GAL4hh.PU, dlg1GD4689
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of neoplasia | larval stage phenotype of BacA\p35UAS.cHa, Bub3GD9924, Scer\GAL4ap-md544
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of increased cell number | larval stage phenotype of BacA\p35UAS.cHa, Bub3GD9924, Scer\GAL4ap-md544
Ecol\lexAGMR15B03/diaRNAi.lexAop is a suppressor of wing disc phenotype of EgfrUAS.cBa, Scer\GAL4ap-md544, psqKK111691
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of wing disc phenotype of BacA\p35UAS.cHa, Bub3GD9924, Scer\GAL4ap-md544
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of myoblast | increased number | larval stage phenotype of BacA\p35UAS.cHa, Bub3GD9924, Scer\GAL4ap-md544
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of wing disc phenotype of BacA\p35UAS.cHa, Scer\GAL4hh.PU, rodGD8768
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of wing disc phenotype of Ras85DV12.UAS, Scer\GAL4ap-md544, dlg1GD4689
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of myoblast | increased number | larval stage phenotype of Ras85DV12.UAS, Scer\GAL4ap-md544, dlg1GD4689
rprlexAop.cSa/Ecol\lexAGMR15B03 is a non-suppressor of wing disc phenotype of Ras85DV12.UAS, Scer\GAL4hh.PU, dlg1GD4689
rprUAS.C, rprlexAop.cSa, Ecol\lexAGMR15B03, Scer\GAL4He.PZ is a non-suppressor of wing disc phenotype of Ras85DV12.UAS, Scer\GAL4hh.PU, dlg1GD4689
The co-expression of rodGD8768 and BacA\p35UAS.cHa under the control of either Scer\GAL4ap-md544 or Scer\GAL4hh.PU induces wing disc tumor-like growth. Myoblast ablation (by expressing rprlexAop.cSa under the control of Ecol\lexAGMR15B03) does not suppress the tumor-like growth.
The co-expression of Ras85DV12.UAS and dlg1GD4689 under the control of Scer\GAL4ap-md544 induces wing disc neoplasia. Myoblast ablation on the host (by expressing rprlexAop.cSa under the control of Ecol\lexAGMR15B03) does not suppress the tumor-like growth.
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.