FlyBase Allele Report: Dmel\plum[Δ1]

General Information

Symbol

Dmel\plum^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0288228

Feature type

allele

Associated gene

Dmel\plum

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Yu et al., 2013)

Mutagen

FLPase

(Yu et al., 2013, Yu et al., 2013)

Progenitor genotype

P{XP}CG5455^d09586

(Yu et al., 2013)

PBac{WH}f05453

(Yu et al., 2013)

Cytology

Description

Scer\FRT-mediated recombination between the two progenitor insertions have resulted in the deletion of genomic sequence including most of plum.

(Yu et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:26,461,413..26,529,454 [-]

Comment:

Deletion resulting from FLP-catalyzed recombination using the FRT-containing transposons PBac{WH}f05453 and P{XP}CG5455^d09586.

(Yu et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body beta'-lobe

(Marmor-Kollet et al., 2019)

adult mushroom body beta-lobe

(Marmor-Kollet et al., 2019)

adult mushroom body beta-lobe | cell non-autonomous | somatic clone

(Marmor-Kollet et al., 2019)

alpha/beta Kenyon cell | adult stage

(Marmor-Kollet et al., 2019)

alpha/beta Kenyon cell | adult stage | cell non-autonomous | somatic clone

(Marmor-Kollet et al., 2019)

axon | adult stage

(Meltzer et al., 2019)

axon | adult stage | cell non-autonomous | somatic clone

(Marmor-Kollet et al., 2019)

embryonic/larval neuromuscular junction | larval stage

(Yu et al., 2013, Yu et al., 2013)

gamma Kenyon cell | adult stage | somatic clone

(Meltzer et al., 2019)

gamma Kenyon cell | somatic clone

(Yu et al., 2013)

gamma Kenyon cell | somatic clone, with Scer\GAL4^Tab2-201Y, plum^KK103873

(Yu et al., 2013)

larval transverse nerve

(Yu et al., 2013, Yu et al., 2013)

Detailed Description

Statement

Reference

plum^Δ1 homozygotes show adult mushroom body defects:

late-born β/β'-axons over-extend beyond the midline in about half brains and often the β/β'-lobes from both hemispheres seem to fuse; γ-neurons show severe pruning defects.

plum^V11X homozygous β-axons only over-extend beyond the midline if in a plum^V11X homozygous background, but not in a heterozygous background.

(Marmor-Kollet et al., 2019)

plum^Δ1 mushroom body gamma neuron clones frequently show pruning defects.

(Meltzer et al., 2019)

The transverse nerve makes inappropriate connections at the neuromuscular junction in plum^Δ1 larvae.

(Yu et al., 2013)

Homozygous mushroom body neuroblast clones show a severe pruning defect at the pupal stage.

8% of homozygous single cell mushroom body neuroblast clones in an otherwise heterozygous background fail to prune their branches, retaining their larval-specific dorsal axon branches into the adult stage. The fraction of unpruned single cell clones is increased if the homozygous plum^Δ1 cells are also expressing plum^KK103873 under the control of Scer\GAL4^Tab2-201Y.

The frequency of improper, ectopic projections at the larval neuromuscular junction is increased compared to wild-type controls, with the majority of the ectopic connections being made by the transverse nerve.

(Yu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

plum^Δ1 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa

(Marmor-Kollet et al., 2019)

plum^Δ1 has abnormal size | adult stage phenotype, suppressible | partially by Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa

(Marmor-Kollet et al., 2019)

plum^Δ1 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by Scer\GAL4^Tab2-201Y/babo^Q302D.UAS

(Yu et al., 2013)

plum^Δ1 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible | partially by Scer\GAL4^Tab2-201Y/Smox^SDVD.UAS

(Yu et al., 2013)

plum^Δ1 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by Scer\GAL4^Tab2-201Y/EcR^B1.UAS

(Yu et al., 2013)

plum^Δ1 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by babo^UAS.cBa/Scer\GAL4^Tab2-201Y

(Yu et al., 2013)

plum^Δ1/plum^Δ2 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/EcR^B1.UAS

(Yu et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

plum^Δ1 has adult mushroom body beta-lobe phenotype, suppressible | partially by Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa

(Marmor-Kollet et al., 2019)

plum^Δ1 has adult mushroom body beta'-lobe phenotype, suppressible | partially by Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa

(Marmor-Kollet et al., 2019)

plum^Δ1 has alpha/beta Kenyon cell | adult stage phenotype, suppressible | partially by Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa

(Marmor-Kollet et al., 2019)

plum^Δ1 has gamma Kenyon cell | somatic clone phenotype, suppressible by Scer\GAL4^Tab2-201Y/babo^Q302D.UAS

(Yu et al., 2013)

plum^Δ1 has gamma Kenyon cell | somatic clone phenotype, suppressible | partially by Scer\GAL4^Tab2-201Y/Smox^SDVD.UAS

(Yu et al., 2013)

Scer\GAL4^Tab2-201Y, plum^Δ1 has gamma Kenyon cell | somatic clone phenotype, suppressible by EcR^B1.UAS, Scer\GAL4^Tab2-201Y

(Yu et al., 2013)

plum^Δ1 has gamma Kenyon cell | somatic clone phenotype, suppressible by babo^UAS.cBa/Scer\GAL4^Tab2-201Y

(Yu et al., 2013)

plum^Δ1/plum^Δ2 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/EcR^B1.UAS

(Yu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of babo^{Q302D.Scer\UAS} under the control of Scer\GAL4^Tab2-201Y rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of Smox^{SDVD.Scer\UAS} under the control of Scer\GAL4^Tab2-201Y partially rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

(Yu et al., 2013)

Expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^Tab2-201Y largely rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of babo^Scer\UAS.cBa under the control of Scer\GAL4^Tab2-201Y rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^elav.PU partially rescues the formation of ectopic connections at the neuromuscular junction in plum^Δ1/plum^Δ2 larvae.

(Yu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

plum^Δ1 is rescued by Scer\GAL4^Tab2-201Y/plum^UAS.Tag:FLAG

(Yu et al., 2013)

plum^Δ1 is rescued by Scer\GAL4^Tab2-201Y/plum^UAS.Tag:HA

(Yu et al., 2013)

plum^Δ1 is rescued by Scer\GAL4^Tab2-201Y/plum^{ΔIg1-3.UAS.Tag:FLAG}

(Yu et al., 2013)

plum^Δ1 is rescued by Scer\GAL4^Tab2-201Y/plum^{ΔCyt.UAS.Tag:FLAG}

(Yu et al., 2013)

plum^Δ1 is rescued by plum^{ECD.UAS.Tag:M(mCd8a),Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Yu et al., 2013)

plum^Δ1 is rescued by plum^GFP,Tag:HA

(Yu et al., 2013)

plum^Δ1 is rescued by plum^ΔCyt.Tag:HA

(Yu et al., 2013)

Partially rescued by

plum^Δ1 is partially rescued by plum^UAS.Tag:FLAG/Scer\GAL4^GMR44E04

(Marmor-Kollet et al., 2019)

plum^Δ1 is partially rescued by Scer\GAL4^Tab2-201Y/plum^{ΔIg1-4.UAS.Tag:FLAG}

(Yu et al., 2013)

Not rescued by

plum^Δ1 is not rescued by Scer\GAL4^GMR44E04/plum^{ΔIg1-3.UAS.Tag:FLAG}

(Marmor-Kollet et al., 2019)

plum^Δ1 is not rescued by Scer\GAL4^GMR44E04/plum^{ΔIg1-4.UAS.Tag:FLAG}

(Marmor-Kollet et al., 2019)

plum^Δ1 is not rescued by Scer\GAL4^GMR44E04/plum^{ΔCyt.UAS.Tag:FLAG}

(Marmor-Kollet et al., 2019)

plum^Δ1 is not rescued by plum^{ECD.UAS.Tag:M(mCd8a),Tag:FLAG}/Scer\GAL4^GMR44E04

(Marmor-Kollet et al., 2019)

plum^Δ1 is not rescued by plum^{ΔIg4.UAS.Tag:FLAG}/Scer\GAL4^Tab2-201Y

(Yu et al., 2013)

Comments

Expression of plum^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y fully rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of plum^{ΔIg1-3.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of plum^{ΔIg1-4.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y partially rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of plum^{ΔIg4.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y shows very little ability to rescue the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones (rescue seen in 1 out of 16 cases).

Expression of plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

plum^{T:Avic\GFP,T:Ivir\HA1} rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

plum^{ΔCyt.T:Ivir\HA1} rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of plum^{ECD.Scer\UAS.T:Mmus\Cd8a,T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of plum^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Tab2-201Y in mushroom body neuroblast clones is sufficient to rescue the γ neuron pruning defects seen in plum^Δ1 homozygous brains.

(Yu et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

plum^Δ1

(Draper et al., 2024, Marmor-Kollet et al., 2019, Meltzer et al., 2019, Wang et al., 2019, Yu et al., 2013, Yu et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

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