FB2024_03 , released June 25, 2024
Allele: Dmel\Ns3K350N.UAS.Venus
Open Close
General Information
Symbol
Dmel\Ns3K350N.UAS.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0285388
Feature type
allele
Associated gene
Dmel\Ns3
Associated Insertion(s)
Carried in Construct
P{UAS-YFP-Ns3.K350N}
Key Links
Transgenic product class
dominant_negative_variant
(Hartl et al., 2013)
missense_variant
(Hartl et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Hartl et al., 2013)
missense_variant
(Hartl et al., 2013)
Mutagen
in vitro construct
(Hartl et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-YFP-Ns3.K350N}
Cytology
Description

UAS regulatory sequences drive expression of an antimorphic version of Ns3 which contains the amino acid replacement K350N. The protein is tagged at the N-terminal end with Venus.

(Hartl et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ns3
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Ns3K350N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Ddc.PL results in developmental delay and a reduced adult body weight compared to controls.

      (Hartl et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Ns3K350N.UAS.Venus is partially rescued by Scer\GAL4Ddc.PL/Ns3UAS.Venus

      (Hartl et al., 2013)
      Fails to rescue

      Ns3K350N.UAS.Venus/Scer\GAL4Ddc.PL fails to rescue Ns3G0431

      (Hartl et al., 2013)
      Comments

      Co-expression of Ns3Scer\UAS.T:Avic\GFP-YFP.Venus rescues the developmental delay and a reduced adult body weight caused by expression of Ns3K350N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Ddc.PL.

      Expression of Ns3K350N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Ddc.PL fails to rescue the reduced adult body weight and developmental delay of Ns3G0431 males.

      (Hartl et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ns3K350N.Scer\UAS.T:Avic\GFP-YFP.Venus
      Ns3K350N.UAS.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (1)