FB2024_03 , released June 25, 2024
Allele: Dmel\Ns3UAS.Venus
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General Information
Symbol
Dmel\Ns3UAS.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0221153
Feature type
allele
Associated gene
Dmel\Ns3
Associated Insertion(s)
Carried in Construct
P{UAS-ns3.YFP}
Key Links
Transgenic product class
wild_type
(Kaplan et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kaplan et al., 2008)
Mutagen
in vitro construct
(Kaplan et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-ns3.YFP}
Cytology
Description

UAS regulatory sequences drive expression of the Ns3 open reading frame tagged with Venus fluorescent protein.

(Kaplan et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ns3
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Tub.PU/Ns3UAS.Venus rescues Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4Tub.PU/Ns3UAS.Venus rescues Ns3G0431

      (Kaplan et al., 2008)
      Partially rescues

      Scer\GAL4Ddc.PL/Ns3UAS.Venus partially rescues Ns3K350N.UAS.Venus

      (Hartl et al., 2013)

      Scer\GAL4elav.PLu/Ns3UAS.Venus partially rescues Ns3G0431

      (Kaplan et al., 2008)
      Fails to rescue

      Scer\GAL4l.Trhn.PK/Ns3UAS.Venus fails to rescue Ns3G0431

      (Hartl et al., 2013)

      Scer\GAL471B/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4en-e16E/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4Lsp2.PH/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL42-286/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4repo/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4ChAT.7.4/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4Tdc2.PC/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4386Y/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4dimm-929/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4S3.Hug/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4Ilp2.PR/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4NPF.1/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4NPFR.6.6/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)

      Scer\GAL4Orco.7.087/Ns3UAS.Venus fails to rescue Ns3G0431

      (Kaplan et al., 2008)
      Comments

      Expression of Ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Ddc.PL completely rescues the reduced adult body weight and partially rescues the developmental delay of Ns3G0431 males.

      Co-expression of Ns3Scer\UAS.T:Avic\GFP-YFP.Venus rescues the developmental delay and a reduced adult body weight caused by expression of Ns3K350N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Ddc.PL.

      Expression of Ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4l.Trh.PK fails to rescue the developmental delay and reduced viability of Ns3G0431 males.

      Expression of Ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4ple.PF substantially rescues the developmental delay and reduced viability of Ns3G0431 males.

      (Hartl et al., 2013)

      The developmental delay, reduced viability and growth defects of ns3G0431 females are rescued by expression of ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4tub.

      (Kaplan et al., 2008)

      The developmental delay, reduced viability and growth defects of ns3G0431 males are completely rescued by expression of ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4tub.

      The reduced wing area seen in ns3G0431 flies is not rescued by expression of ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL471B.

      Expression of ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4en-e16E does not increase cell size in the posterior wing of ns3G0431 animals.

      Expression of ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of one of Scer\GAL4Lsp2.PH, Scer\GAL42-286, Scer\GAL4repo, Scer\GAL4Cha.7.4, Scer\GAL4ple.PF, Scer\GAL4Tdc2.PC, Scer\GAL4386Y, Scer\GAL4dimm-929, Scer\GAL4S3.hug, Scer\GAL4Ilp2.PR, Scer\GAL4npf.1, Scer\GAL4NPFR1.6.6 or Scer\GAL4Or83b.7.087 does not rescue the developmental delay or growth defects of ns3G0431 animals.

      Expression of ns3Scer\UAS.T:Avic\GFP-YFP.Venus under the control of one of Scer\GAL4elav.PLu or Scer\GAL4Ddc.PL partially rescues the developmental delay and growth defects of ns3G0431 animals.

      (Kaplan et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ns3Scer\UAS.T:Avic\GFP-YFP.Venus
      Ns3UAS.Venus
      ns3Scer\UAS.T:Avic\GFP-YFP.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (4)