FB2024_03 , released June 25, 2024
Allele: Dmel\gishMI08417
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General Information
Symbol
Dmel\gishMI08417
Species
D. melanogaster
Name
FlyBase ID
FBal0284858
Feature type
allele
Associated gene
Dmel\gish
Associated Insertion(s)
Mi{MIC}gishMI08417
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    Mi{MIC}gishMI08417
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    Mi{MIC}
    Also carries
    attP
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        gish04895/gishMI08417 as well as gishMI08417/gishKG03891 transheterozygous mutant males show decreased average number of germline stem cells in their testes already at post-eclosion day 1 and as the flies age the GSCs continue to be lost at a rapid rate, this however is not due to excessive apoptosis and neither do the mutant GSCs lose their adhesion to the hub cells as shown in gish04895/gishMI08417 or gishMI08417/gishKG03891 males.

        No significant difference in the number of GSCs is observed in gishMI08417 heterozygotes, as compared to wild-type controls.

        The germline stem cells in the heat-shock induced gishMI08417 mutant germline clones in testes of adult male flies are rapidly lost as the flies age.

        (Chen et al., 2017)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        gish04895/gishMI08417 is rescued by gishgish.F

        (Chen et al., 2017)

        gish04895/gishMI08417 is rescued by gishUASp.F/Scer\GAL4VP16.nanos.UTR

        (Chen et al., 2017)

        gishMI08417/gishKG03891 is rescued by gishgish.F

        (Chen et al., 2017)

        gishMI08417/gishKG03891 is rescued by gishnanos.F

        (Chen et al., 2017)

        gishMI08417/gishKG03891 is rescued by gishUASp.F/Scer\GAL4VP16.nanos.UTR

        (Chen et al., 2017)
        Not rescued by

        gish04895/gishMI08417 is not rescued by gishUASp.F/Scer\GAL4C587

        (Chen et al., 2017)
        Comments

        The age-progressive loss of germline stem cells in adult male testes observed in gish04895/gishMI08417 or gishMI08417/gishKG03891 transheterozygotes is fully rescued by combination with gishgish.F. Similarly, combination with gishnos.F also rescues the phenotype in gishMI08417/gishKG03891 mutants and both gish04895/gishMI08417 and gish04895/gishMI08417 mutants can be rescued by expression of gishScer\UAS.P\T.F under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 (but not Scer\GAL4C587).

        (Chen et al., 2017)
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        gishMI08417
        (Chen et al., 2017, Bellen, 2013.6.4)
        Name Synonyms
        Secondary FlyBase IDs
          References (2)