FlyBase Allele Report: Dmel\gish[KG03891]

General Information

Symbol

Dmel\gish^KG03891

Species

D. melanogaster

Name

FlyBase ID

FBal0130262

Feature type

allele

Associated gene

Dmel\gish

Associated Insertion(s)

P{SUPor-P}gish^KG03891

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}gish^KG03891

Cytology

Description

Insertion in the third exon.

(Tan et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,281,016..16,281,016 [-]

(Li et al., 2020, Howlett et al., 2013, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | dominant

(Tan et al., 2010)

decreased cell number | adult stage | progressive (with gish⁰⁴⁸⁹⁵)

(Chen et al., 2017)

decreased cell number | adult stage | progressive (with gish^MI08417)

(Chen et al., 2017)

male sterile

(Tan et al., 2010)

partially lethal - majority die | recessive

(Tan et al., 2010)

Phenotype Manifest In

male germline stem cell | adult stage | progressive (with gish⁰⁴⁸⁹⁵)

(Chen et al., 2017)

male germline stem cell | adult stage | progressive (with gish^MI08417)

(Chen et al., 2017)

Detailed Description

Statement

Reference

gish⁰⁴⁸⁹⁵/gish^KG03891 as well as gish^MI08417/gish^KG03891 transheterozygous mutant males show decreased average number of germline stem cells in their testes already at post-eclosion day 1 and as the flies age the GSCs continue to be lost at a rapid rate, this however is not due to excessive apoptosis and neither do the mutant GSCs lose their adhesion to the hub cells as shown in gish^MI08417/gish^KG03891 males.

No significant difference in the number of GSCs is observed in gish^KG03891 heterozygotes, as compared to wild-type controls.

(Chen et al., 2017)

Homozygotes show strong, but incomplete lethality.

Heterozygotes show a significant impairment in 3 minute, 30 minute and 3 hour memory after olfactory conditioning compared to wild-type flies.

Heterozygotes and homozygotes show normal shock and odour avoidance.

(Tan et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

gish^KG03891/gish^KG03891 is an enhancer | somatic clone of visible phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

NOT Suppressor of

Statement

Reference

gish[+]/gish^KG03891 is a non-suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

gish[+]/gish^KG03891 is a non-suppressor of bang sensitive | semidominant phenotype of para^bss1

(Howlett et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

gish^KG03891/gish^KG03891 is an enhancer | somatic clone of wing phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

NOT Suppressor of

Statement

Reference

gish[+]/gish^KG03891 is a non-suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

gish^KG03891/+ does not modify the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096, but the presence of gish^KG03891/gish^KG03891 clones in the wings of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 leads to a greatly enhanced fused wing phenotype.