FlyBase Allele Report: Dmel\PRL-1[UAS.cPa]

General Information

Symbol

Dmel\PRL-1^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pagarigan

FlyBase ID

FBal0284665

Feature type

allele

Associated gene

Dmel\PRL-1

Associated Insertion(s)

Carried in Construct

P{UAS-PRL-1.P}

Also Known As

UAS-PRL-1

Key Links

Transgenic product class

cDNA

(Pagarigan et al., 2013)

wild_type

(Pagarigan et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pagarigan et al., 2013)

wild_type

(Pagarigan et al., 2013)

Mutagen

in vitro construct

(Pagarigan et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-PRL-1.P}

Cytology

Description

UASt regulatory sequences drive expression of a full-length PRL-1 cDNA (LD12894).

(Pagarigan et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

PRL-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by Src64B^UY1332

(Pagarigan et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head capsule | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Pagarigan et al., 2013)

axon | adult stage, with Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

eye | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Pagarigan et al., 2013)

mechanosensory neuron | adult stage, with Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

neuron projection | adult stage | ectopic, with Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

synapse | increased number, with Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

wing, with Scer\GAL4^en-e16E

(Pagarigan et al., 2013)

wing disc | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Pagarigan et al., 2013)

Detailed Description

Statement

Reference

In adult mechanosensory neurons expressing PRL-1^UAS.cPa under the control of Scer\GAL4^pnr-MD237, axon terminals appear less mature than controls but do not show significant changes in terminal arbor territory; axons show small protrusions on the main axon branch that show significantly more synapses (Syt1 puncta), and even longer filopodium-like protrusions in terminal region, which are not detected in controls.

Posterior dorsocentral bristle neurons of individuals that are both PRL-1²/PRL-1⁴ transheterozygous and express PRL-1^UAS.cPa under the control of Scer\GAL4^pnr-MD237 do not show significant projection defects (terminal arbor territory or number of synapses/ Syt1-positive puncta) in the contralateral branch, as compared to controls.

(Urwyler et al., 2019)

Expression of PRL-1^Scer\UAS.cPa in the posterior compartment of the wing disc under the control of Scer\GAL4^en-e16E reduces the surface area of the adult wing by 20%. Expression in the dorsal compartment using Scer\GAL4^ap-md544 leads to an upwards curvature, which is also indicative of a decrease in surface area.

Expression of PRL-1^Scer\UAS.cPa in the developing eye (using Scer\FLP1^ey.4x.Exel under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) leads to a smaller eye and head capsule.

Wing disc clones expressing PRL-1^Scer\UAS.cPa (under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C}) contain fewer cells than controls.

Ubiquitous expression of PRL-1^Scer\UAS.cPa under the control of Scer\GAL4^Act.PU prevents larval growth; although the larvae consume food, most stall in the first instar larval stages of development for 2-6 days before dying. The larvae do not exhibit any obvious morphological defects.

Flies co-expressing PRL-1^Scer\UAS.cPa and PRL-1^Scer\UAS.NC in the dorsal compartment of the wing disc under the control of Scer\GAL4^ap-md544 do not display any wing curvature.

(Pagarigan et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^pnr-MD237, sktl^UAS.cHa

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^pnr-MD237, sktl^UAS.cHa

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^ap-md544 is a suppressor | partially of lethal - all die before end of pupal stage phenotype of Scer\GAL4^ap-md544, Src64B^UY1332

(Pagarigan et al., 2013)

PRL-1^UAS.cPa, Scer\GAL4^ap-md544 is a suppressor | partially of hyperplasia | larval stage phenotype of Scer\GAL4^ap-md544, Src64B^UY1332

(Pagarigan et al., 2013)

PRL-1^UAS.cPa, Scer\GAL4^ap-md544 is a suppressor | partially of increased cell death | larval stage phenotype of Scer\GAL4^ap-md544, Src64B^UY1332

(Pagarigan et al., 2013)

NOT Suppressor of

Statement

Reference

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Akt^HM04007, Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a non-suppressor of abnormal size | adult stage phenotype of Akt^HM04007, Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

Other

Statement

Reference

PRL-1^UAS.cPa, Ras85D^V12.UAS, Scer\GAL4^ap-md544 has decreased rate of movement | larval stage phenotype

(Pagarigan et al., 2013)

PRL-1^UAS.cPa, Ras85D^V12.UAS, Scer\GAL4^ap-md544 has lethal - all die before end of prepupal stage phenotype

(Pagarigan et al., 2013)

PRL-1^UAS.cPa, Scer\GAL4^ap-md544, Src64B^UY1332 has some die during pupal stage phenotype

(Pagarigan et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a suppressor of mechanosensory neuron of posterior dorsocentral bristle phenotype of Scer\GAL4^pnr-MD237, sktl^UAS.cHa

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a suppressor of neuron projection | adult stage phenotype of Scer\GAL4^pnr-MD237, sktl^UAS.cHa

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^ap-md544 is a suppressor of wing disc phenotype of Scer\GAL4^ap-md544, Src64B^UY1332

(Pagarigan et al., 2013)

NOT Suppressor of

Statement

Reference

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a non-suppressor of mechanosensory neuron of posterior dorsocentral bristle phenotype of Akt^HM04007, Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a non-suppressor of neuron projection | adult stage phenotype of Akt^HM04007, Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

PRL-1^UAS.cPa, Scer\GAL4^pnr-MD237 is a non-suppressor of synapse | adult stage phenotype of Akt^HM04007, Scer\GAL4^pnr-MD237

(Urwyler et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of PRL-1^Scer\UAS.cPa partially suppresses the pupal lethality seen when Src64B^UY1332 is expressed in the dorsal compartment of the wing disc under the control of Scer\GAL4^ap-md544, enabling 45% of the expected adults to eclose. The overgrowth and apoptosis seen in the larval wing discs are also suppressed by PRL-1^Scer\UAS.cPa.

Expression of PRL-1^Scer\UAS.cPa enhances the lethality seen when Ras85D^V12.Scer\UAS is expressed in the dorsal compartment of the wing disc under the control of Scer\GAL4^ap-md544. Fewer than 10% of mutants reach the prepupal stage, compared to 90% when Ras85D^V12.Scer\UAS is expressed alone. The larvae appear lethargic.

(Pagarigan et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/PRL-1^UAS.cPa rescues PRL-1^LL07771

(Kula-Eversole et al., 2021)

PRL-1^UAS.cPa/Scer\GAL4^pnr-MD237 rescues PRL-1²

(Urwyler et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PRL-1^Scer\UAS.cPa

PRL-1^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pagarigan

Secondary FlyBase IDs

References (3)

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