lethal, with Scer\GAL4ey.PH
eye, with Scer\GAL4ey.PH
Expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4ey.PH results in the lack of eclosion of flies. When flies are removed from their pupal casings, they are found arrested at different developmental stages ranging from early pupae to almost fully developed flies. As a result, some cephalic structures consist of only the presumptive proboscis still attached to the larval mouth hook, while others are fully formed though lacking any eyes. Transgenic lines expressing Ivir\M2H37A.Scer\UAS at lower level verify that the toxin is working specifically to ablate the eyes. A small number of flies from these crosses are able to eclose and they display a total absence of eye tissue, reduced eyes, and/or cyclopia.
Expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4msn.PL results in the ablation of almost all lamellocytes. Those lamellocytes that remain are aggregated into large melanotic masses in third instar larvae.
Expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4He.PZ results in a significant reduction in hemocyte cells, including ablation of the salivary glands in all third instar larvae and melanotic masses in the hemolymph that attached to organelles in roughly half of the total population screened.
Scer\GAL4HCH.Hand>Ivir\M2H37A.Scer\UAS induced cell death does not commence until mid to late second instar larval stage. Scer\GAL4HCH.Hand>Ivir\M2H37A.Scer\UAS-expressing adult flies display zero to one pericardial cell associated with the dorsal vessel. Expression of Scer\GAL4HCH.Hand>Ivir\M2H37A.Scer\UAS results in the specific ablation of excretory cells (pericardiac nephrocytes) that flank the dorsal vessel. These flies die prematurely compared with controls.
Analysis of the phase of lethality resulting from the expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4HCH.Hand indicates no significant death (as compared with controls) during embryogenesis, larval or pupal stages.
No ablation of Scer\GAL4elav-C155>Ivir\M2H37A.Scer\UAS-expressing nervous system cells is observed during embryonic stages. Ablation of cells only begins to be apparent during the middle of the second instar larval stage.
Ivir\M2H37A.Scer\UAS expresses a toxic ion channel protein that can be used in combination with a Scer\GAL4 driver for specific cell ablation.