FlyBase Allele Report: Dmel\rg[UAS.cVa]

General Information

Symbol

Dmel\rg^UAS.cVa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct of Volders

FlyBase ID

FBal0281963

Feature type

allele

Associated gene

Dmel\rg

Associated Insertion(s)

Carried in Construct

P{UAS-rg.V}

Key Links

Transgenic product class

cDNA

(Volders et al., 2012)

wild_type

(Volders et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Volders et al., 2012)

wild_type

(Volders et al., 2012)

Mutagen

in vitro construct

(Volders et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-rg.V}

Cytology

Description

UASt regulatory sequences drive expression of a full-length 10.5kb rg cDNA.

(Volders et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^n-syb.PS has no effect on bouton number at the neuromuscular junction in third instar larvae.

(Volders et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/rg^UAS.cVa rescues rg^γ5

(Volders et al., 2012)

Scer\GAL4^ey-OK107/rg^UAS.cVa rescues rg^γ5

(Volders et al., 2012)

Scer\GAL4^elav.PU/rg^UAS.cVa rescues rg¹

(Volders et al., 2012)

rg^UAS.cVa/Scer\GAL4^GMR.PU rescues rg^FDD

(Volders et al., 2012)

rg^UAS.cVa/Scer\GAL4^nSyb.PS rescues rg^FDD

(Volders et al., 2012)

Partially rescues

Scer\GAL4^Mef2.247/rg^UAS.cVa partially rescues rg^γ5

(Volders et al., 2012)

Scer\GAL4^elav.PU/rg^UAS.cVa partially rescues rg^FDD

(Volders et al., 2012)

Scer\GAL4^ey-OK107/rg^UAS.cVa partially rescues rg^FDD

(Volders et al., 2012)

Fails to rescue

Scer\GAL4^GH298/rg^UAS.cVa fails to rescue rg^γ5

(Volders et al., 2012)

rg^UAS.cVa/Scer\GAL4^GH146 fails to rescue rg^γ5

(Volders et al., 2012)

Scer\GAL4^Mef2.247/rg^UAS.cVa fails to rescue rg^FDD

(Volders et al., 2012)

Comments

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^GMR.PU rescues the rough eye phenotype of rg^FDD flies.

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive and appetitive memory seen in rg¹ flies in an olfactory conditioning assay.

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive and appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay. If expression of rg^Scer\UAS.cVa is restricted to the adult stage 36 hours before the olfactory conditioning assay (using the temperature-sensitive Scer\GAL80^ts.αTub84B allele), the rescue of aversive memory is abolished.

Expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^ey-OK107 rescues the loss of immediate aversive and appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay.

The mushroom body defects seen in hemizygous rg^γ5 males are rescued by expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ey-OK107, but are not rescued by expression under the control of Scer\GAL4^Mef2.247.

Expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^GH298 or Scer\GAL4^GH146 fails to rescue the loss of immediate aversive and appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay.

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive memory seen in rg^FDD flies and partially rescues the loss of immediate appetitive memory seen in rg^FDD flies in an olfactory conditioning assay.

The mushroom body defects seen in hemizygous rg^FDD males are partially rescued by expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ey-OK107, but are not rescued by expression under the control of Scer\GAL4^Mef2.247.

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^n-syb.PS rescues the increased bouton number at the neuromuscular junction which is seen in hemizygous rg^FDD male third instar larvae.

(Volders et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78793

w^* rg^γ5; P{UAS-rg.V}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

rg^Scer\UAS.cVa

rg^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Volders

Secondary FlyBase IDs

References (3)

Report Sections

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