FB2024_03 , released June 25, 2024
Allele: Mmus\Plk1UASp.GFP
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General Information
Symbol
Mmus\Plk1UASp.GFP
Species
M. musculus
Name
FlyBase ID
FBal0281051
Feature type
allele
Associated gene
Mmus\Plk1
Associated Insertion(s)
Carried in Construct
P{UASp-Plk1.GFP}
Transgenic product class
wild_type
(Pearson et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Pearson et al., 2006)
Mutagen
in vitro construct
(Pearson et al., 2006)
Progenitor genotype
Carried in construct
P{UASp-Plk1.GFP}
Cytology
Description

UASp regulatory sequences drives expression of Mmus\Plk1 which is tagged at the N-terminal end with GFP.

(Pearson et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Mmus\Plk1
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos expressing Mmus\Plk1Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 show mitotic defects. Cycle 10-11 embryos show mitotic defects in 4.8% of nuclei, while cycle 12-13 embryos show defects in 22.4% of nuclei. In the 12th mitosis, chains of interconnected nuclei and free centrosomes are seen. The actin cytoskeleton is disorganised.

      (Pearson et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Mmus\Plk1UASp.GFP/Scer\GAL4VP16.mat.αTub67C is an enhancer of lethal phenotype of poloS025604

      (Pearson et al., 2006)
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Mmus\Plk1Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 does not rescue the sterility of polo1/poloS025604 females.

      The majority of poloS025604 homozygotes expressing Mmus\Plk1Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 only survive to the second larval instar stage, in contrast to poloS025604 single homozygotes which survive to the third larval instar stage.

      (Pearson et al., 2006)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mmus\Plk1Scer\UAS.P\T.T:Avic\GFP
      Mmus\Plk1UASp.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)