FlyBase Allele Report: Scer\GAL4[bun-GSG5961]

General Information

Symbol

Scer\GAL4^bun-GSG5961

Species

S. cerevisiae

Name

FlyBase ID

FBal0280521

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{Switch2}bun^GSG5961

Carried in Construct

Also Known As

GS5961, 5961GS, pSwitch^AMP, 5961-GS

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{Switch2}bun^GSG5961

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{Switch2}

enhancer trap

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

midgut imaginal island

(Mathur et al., 2010)

adult stage

intestinal stem cell

(Filer et al., 2017, Foronda et al., 2014)

Additional Information

Statement

Reference

ScerGAL4^bun-GSG5961 drives mifepristone-inducible expression in adult intestinal stem cells.

(Foronda et al., 2014)

ScerGAL4^bun-GSG5961 drives mifepristone-inducible expression primarily in neurons. Weak expression is observed in 2-5 lateral cells/hemisegment in the ventral nerve cord. Strong expression is obversed in a small ventral cluster of neurons in the brain that form projections surrounding the brain midline. Moderate expression is observed in the optic lobe primodia; weak expression is observed in a few scattered cells that may be glia. In the PNS, strong expression is observed in class I dendritic arborizing neuron (type I md-da) and abdominal ventral bipolar neuron vbp (vbd); weak expression is observed in a subset of external sensory and chordotonal organs.

(Nicholson et al., 2008)

Marker for

midgut imaginal island

(Mathur et al., 2010)

Reflects expression of

bun

(Mathur et al., 2010, Nicholson et al., 2008)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | adult stage, with Nrg^HMS01638

(Resnik-Docampo et al., 2021)

decreased occurrence of cell division | adult stage, with Polr3A^GD4257

(Filer et al., 2017)

decreased occurrence of cell division | adult stage | RU486 conditional, with Akt^GD1361

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage | RU486 conditional, with bsk^GD10555

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage | RU486 conditional, with bsk^K53R.UAS

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage | RU486 conditional, with chico¹

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage | RU486 conditional, with InR^K1409A.UAS

(Biteau et al., 2010)

decreased occurrence of cell division | adult stage | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Biteau et al., 2010)

increased cell number | adult stage, with Hsap\L1CAM^UAS.cGa

(Resnik-Docampo et al., 2021)

increased cell number | adult stage, with Nrg^167.UAS

(Resnik-Docampo et al., 2021)

increased occurrence of cell division | adult stage, with btl::Egfr^λ.UAS

(Resnik-Docampo et al., 2021)

increased occurrence of cell division | adult stage, with Hsap\L1CAM^UAS.cGa

(Resnik-Docampo et al., 2021)

increased occurrence of cell division | adult stage, with Nrg^167.UAS

(Resnik-Docampo et al., 2021)

long lived, with PEK^GD5584

(Wang et al., 2015)

long lived, with Polr3A^GD4257

(Filer et al., 2017)

long lived, with srl^UAS.cRa

(Rera et al., 2011)

long lived, with Xbp1^UAS.RB

(Wang et al., 2015)

long lived | RU486 conditional, with Akt^GD1361

(Biteau et al., 2010)

long lived | RU486 conditional, with bsk^GD10555

(Biteau et al., 2010)

long lived | RU486 conditional, with bsk^K53R.UAS

(Biteau et al., 2010)

long lived | RU486 conditional, with InR^K1409A.UAS

(Biteau et al., 2010)

long lived | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Biteau et al., 2010)

long lived | RU486 conditional, with srl^UAS.cRa

(Rera et al., 2011)

Phenotype Manifest In

adult gut | RU486 conditional, with Akt^GD1361

(Biteau et al., 2010)

adult gut | RU486 conditional, with bsk^GD10555

(Biteau et al., 2010)

adult gut | RU486 conditional, with bsk^K53R.UAS

(Biteau et al., 2010)

adult gut | RU486 conditional, with InR^K1409A.UAS

(Biteau et al., 2010)

adult gut | RU486 conditional, with Pi3K92E^{D954A.UAS.Tag:MYC}

(Biteau et al., 2010)

adult midgut epithelium, with Polr3A^GD4257

(Filer et al., 2017)

adult midgut progenitor cell | late third instar larval stage, with N^{Intra1768.UAS.Tag:FLAG}

(Mathur et al., 2010)

adult posterior midgut epithelium, with btl::Egfr^λ.UAS

(Resnik-Docampo et al., 2021)

adult posterior midgut epithelium, with Hsap\L1CAM^UAS.cGa

(Resnik-Docampo et al., 2021)

adult posterior midgut epithelium, with Nrg^167.UAS

(Resnik-Docampo et al., 2021)

adult posterior midgut epithelium, with Nrg^HMS01638

(Resnik-Docampo et al., 2021)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS has increased occurrence of cell division | adult stage phenotype, non-enhanceable by Nrg^HMS01638, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Suppressed by

Statement

Reference

Nrg^167.UAS, Scer\GAL4^bun-GSG5961 has increased occurrence of cell division | adult stage phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

NOT suppressed by

Statement

Reference

Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS has increased occurrence of cell division | adult stage phenotype, non-suppressible by Nrg^HMS01638, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

NOT Enhancer of

Statement

Reference

Nrg^HMS01638, Scer\GAL4^bun-GSG5961 is a non-enhancer of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS

(Resnik-Docampo et al., 2021)

Suppressor of

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961 is a suppressor of increased occurrence of cell division | adult stage phenotype of Nrg^167.UAS, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, BacA\p35^UAS.cUa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, BacA\p35^UAS.cUa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

NOT Suppressor of

Statement

Reference

Nrg^HMS01638, Scer\GAL4^bun-GSG5961 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS

(Resnik-Docampo et al., 2021)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS has adult posterior midgut epithelium phenotype, non-enhanceable by Nrg^HMS01638, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Suppressed by

Statement

Reference

Nrg^167.UAS, Scer\GAL4^bun-GSG5961 has adult posterior midgut epithelium phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

NOT suppressed by

Statement

Reference

Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS has adult posterior midgut epithelium phenotype, non-suppressible by Nrg^HMS01638, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

NOT Enhancer of

Statement

Reference

Nrg^HMS01638, Scer\GAL4^bun-GSG5961 is a non-enhancer of adult posterior midgut epithelium phenotype of Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS

(Resnik-Docampo et al., 2021)

Suppressor of

Statement

Reference

Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961 is a suppressor of adult posterior midgut epithelium phenotype of Nrg^167.UAS, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, BacA\p35^UAS.cUa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional | cell non-autonomous of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

NOT Suppressor of

Statement

Reference

Nrg^HMS01638, Scer\GAL4^bun-GSG5961 is a non-suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^bun-GSG5961, btl::Egfr^λ.UAS

(Resnik-Docampo et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pswitch^AMP

(Mathur et al., 2010)

Scer\GAL4^GSG5961

Scer\GAL4^Switch1.AMP

Scer\GAL4^bun-GSG5961

Name Synonyms

Secondary FlyBase IDs

FBal0243218
FBal0220217

References (18)

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