FB2024_03 , released June 25, 2024
Allele: Dmel\cosH29
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General Information
Symbol
Dmel\cosH29
Species
D. melanogaster
Name
FlyBase ID
FBal0278219
Feature type
allele
Associated gene
Dmel\cos
Associated Insertion(s)
Carried in Construct
Also Known As
cos2H29
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
    Nature of the Allele
    Allele class

    amorphic allele - molecular evidence

    (Christiansen et al., 2013)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Amino acid replacement: R15term.

    (Christiansen et al., 2013)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2R:7,386,937..7,386,937 [-]
    Nucleotide change:

    A7386937T

    Amino acid change:

    R15term | cos-PA; R15term | cos-PB

    Reported amino acid change:

    R15term

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    (Christiansen et al., 2013)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        cosH29 homozygous follicle cell clones show an excess of polar/stalk cells, as compared to controls; clones in the germarium, however, are of comparable sizes to control clones.

        (Dai et al., 2017)

        Homozygous clones in the eye induced using the ey-FLP/FRT system result in overgrowth of the eye. Most of the overgrown tissue is cos[+], suggesting that the clone acts non-autonomously (which is confirmed by analysing clones in the eye disc). Overgrowth is also seen in the head cuticle and the antennae.

        Homozygous clones in the eye disc that cross the morphogenetic furrow (MF) boundary are associated with additional BrdU incorporation at the boundary of the clone. Within mutant clones anterior to the MF, there is a marked decrease in BrdU incorporation compared to controls.

        Homozygous clones in the eye induced using the ey-FLP cell-lethal method result in eyes that are smaller than normal. Overgrowth of the head cuticle and antenna is seen in these animals.

        (Christiansen et al., 2012)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Suppressed by
        Suppressor of
        Statement
        Reference

        cosH29 is a suppressor of increased cell death phenotype of hidGMR.PG

        (Christiansen et al., 2013)
        Phenotype Manifest In
        Suppressed by
        Suppressor of
        Statement
        Reference

        cosH29 is a suppressor of eye phenotype of hidGMR.PG

        (Christiansen et al., 2013)
        Other
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Eyes mosaic for cosH29 (generated using the ey-FLP/FRT system) moderately suppress the WGMR.PG eye phenotype. This suppression is cell non-autonomous.

        Eye discs mosaic for cosH29 (generated using the ey-FLP/FRT system) suppress the WGMR.PG cell death phenotype by increasing the apoptosis resistance of neighbouring non-mutant cells - cosH29 clones themselves are unprotected from WGMR.PG-induced apoptosis. This cell non-autonomous suppression occurs only in the first, and not the second, apoptotic wave.

        The suppression of the WGMR.PG eye phenotype seen in eyes mosaic for cosH29 (generated using the ey-FLP/FRT system) is reverted by expression of ciCe-2.Scer\UAS.T:Ivir\HA1 (via the MARCM system) in the cosH29 clones.

        The suppression of the WGMR.PG eye phenotype seen in eyes mosaic for cosH29 (generated using the ey-FLP/FRT system) is abrogated by heterozygosity for Df(1)N-8, or both DlRevF10 and SerRX82.

        (Christiansen et al., 2013)

        Expression of ci75.Scer\UAS.cKa under the control of Scer\GAL4tub.PU in cosH29 clones in the eye suppresses the overgrowth phenotype caused by the cosH29 clones.

        (Christiansen et al., 2012)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        Cos2H29
        (Moore et al., 2022)
        cos2H29
        (Christiansen et al., 2013, Christiansen et al., 2012)
        cosH29
        (Dai et al., 2017)
        Name Synonyms
        Secondary FlyBase IDs
          References (4)