UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing an miRNA cluster (includes the mir-310, mir-311, mir-312 and mir-313 pre-miRNA hairpins) has been inserted into the DsRed(Unk) 3'UTR.
Scer\GAL4unspecified, mir-310UAS.cluster.DsRed(Unk), mir-311UAS.cluster.DsRed(Unk), mir-312UAS.cluster.DsRed(Unk), mir-313UAS.cluster.DsRed(Unk) has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, suppressible | partially by BacA\p35UAS.cHa, Scer\GAL4unspecified
Clones expressing P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} (carries mir-310Scer\UAS.cluster.T:Disc\RFP, mir-311Scer\UAS.cluster.T:Disc\RFP, mir-312Scer\UAS.cluster.T:Disc\RFP and mir-313Scer\UAS.cluster.T:Disc\RFP) under the control of Scer\GAL4unspecified are difficult to recover in the wing imaginal disc. Co-expression of armS10.Scer\UAS.T:Hsap\MYC significantly rescues the viability of these clones.
Expression of P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} (carries mir-310Scer\UAS.cluster.T:Disc\RFP, mir-311Scer\UAS.cluster.T:Disc\RFP, mir-312Scer\UAS.cluster.T:Disc\RFP and mir-313Scer\UAS.cluster.T:Disc\RFP) under the control of Scer\GAL4bbg-C96 results in wing notching.
Co-expression of armS10.Scer\UAS.T:Hsap\MYC rescues the wing notching seen in flies expressing P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} (carries mir-310Scer\UAS.cluster.T:Disc\RFP, mir-311Scer\UAS.cluster.T:Disc\RFP, mir-312Scer\UAS.cluster.T:Disc\RFP and mir-313Scer\UAS.cluster.T:Disc\RFP) under the control of Scer\GAL4bbg-C96.
Co-expression of P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} (carries mir-310Scer\UAS.cluster.T:Disc\RFP, mir-311Scer\UAS.cluster.T:Disc\RFP, mir-312Scer\UAS.cluster.T:Disc\RFP and mir-313Scer\UAS.cluster.T:Disc\RFP) suppresses the specification of extra wing margin bristles caused by expression of armS10.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4bbg-C96.
Expression of P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} under the control of Scer\GAL4Bx-MS1096 results in wings which are curved, small and folded.
Co-expression of BacA\p35Scer\UAS.cHa rescues the viability of clones in the wing disc expressing P{UAS-DsRed-mir-310+mir-311+mir-312+mir-313.L} (carries mir-310Scer\UAS.cluster.T:Disc\RFP, mir-311Scer\UAS.cluster.T:Disc\RFP, mir-312Scer\UAS.cluster.T:Disc\RFP and mir-313Scer\UAS.cluster.T:Disc\RFP) under the control of Scer\GAL4unspecified. The recovered clones are small, consisting of just one to three cells and appear to be delaminating from the epithelial plane.