FlyBase Allele Report: Dmel\Med[GD1483]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\Med^GD1483

General Information

Symbol

Dmel\Med^GD1483

Species

D. melanogaster

Name

FlyBase ID

FBal0277891

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

medea RNAi

Key Links

Genomic Maps

Transgenic product class

(Takaesu et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Takaesu et al., 2012)

Mutagen

in vitro construct

(Takaesu et al., 2012)

Progenitor genotype

Carried in construct

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Takaesu et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA with Apc^GD1333, Ras85D^V12.cUa.UAS, p53^GD4483

(Quintero and Bangi, 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^eya.PB

(Richard et al., 2017)

Phenotype Manifest In

eye | decreased number, with Scer\GAL4^eya.PB

(Richard et al., 2017)

Detailed Description

Statement

Reference

Adults expressing Med^GD1483 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Med^GD1483 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD1483} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Med^GD1483, Scer\GAL4^eya.PB has visible | adult stage phenotype, enhanceable by Inx3^RNAi.UAS.WIZ, Scer\GAL4^eya.PB

(Richard et al., 2017)

Suppressed by

Statement

Reference

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has abnormal cell number | adult stage | somatic clone - tissue specific phenotype, suppressible | partially by sc^JF02104, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell growth rate | adult stage | somatic clone - tissue specific phenotype, suppressible by sc^JF02104, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell size | adult stage | somatic clone - tissue specific phenotype, suppressible by sc^JF02104, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

NOT suppressed by

Statement

Reference

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell growth rate | adult stage | somatic clone - tissue specific phenotype, non-suppressible by Tk^JF01818, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has abnormal cell number | adult stage | somatic clone - tissue specific phenotype, non-suppressible by InR^GL00139, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell size | somatic clone - tissue specific phenotype, non-suppressible by InR^GL00139, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has abnormal cell number | adult stage | somatic clone - tissue specific phenotype, non-suppressible by TkR99D^JF02663, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell size | adult stage | somatic clone - tissue specific phenotype, non-suppressible by TkR99D^JF02663, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has abnormal cell number | adult stage | somatic clone - tissue specific phenotype, non-suppressible by Tk^JF01818, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

NOT Enhancer of

Statement

Reference

Scer\GAL80^ts.αTub84B, Med^GD1483, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Med^GD1483, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Other

Statement

Reference

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell size | adult stage | somatic clone - tissue specific phenotype

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased occurrence of cell division | adult stage | somatic clone - tissue specific phenotype

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has short lived | somatic clone - tissue specific phenotype

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has increased cell growth rate | somatic clone - tissue specific phenotype

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has abnormal cell number | adult stage | somatic clone - tissue specific phenotype

(Quintero and Bangi, 2023)

Inx3^RNAi.UAS.WIZ, Med^GD1483, Scer\GAL4^eya.PB has visible | adult stage phenotype

(Richard et al., 2017)

Inx3^RNAi.UAS.WIZ, Med^GD1483, Scer\GAL4^eya.PB has decreased size | adult stage phenotype

(Richard et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Med^GD1483, Scer\GAL4^eya.PB has eye | decreased number phenotype, enhanceable by Inx3^RNAi.UAS.WIZ, Scer\GAL4^eya.PB

(Richard et al., 2017)

Suppressed by

Statement

Reference

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has adult enteroendocrine cell | increased number | somatic clone - tissue specific phenotype, suppressible | partially by sc^JF02104, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

NOT suppressed by

Statement

Reference

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has intestinal stem cell | increased number | cell non-autonomous | adult stage | somatic clone - tissue specific phenotype, non-suppressible by Pten^RNAi.UAS.cUa, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has intestinal stem cell | increased number | cell non-autonomous | adult stage | somatic clone - tissue specific phenotype, non-suppressible by InR^GL00139, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Apc^GD1333, Med^GD1483, Ras85D^V12.cUa.UAS, Scer\GAL4^{Act5C.PS-FRT3}, p53^GD4483 has intestinal stem cell | increased number | cell non-autonomous | adult stage | somatic clone - tissue specific phenotype, non-suppressible by TkR99D^JF02663, Scer\GAL4^{Act5C.PS-FRT3}

(Quintero and Bangi, 2023)

Other

Statement

Reference

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

v19688

w¹¹¹⁸; P{GD1483}v19688

v19689

w¹¹¹⁸; P{GD1483}v19689

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Med^GD1483

medea^dsRNA

(Luo et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections