FlyBase Allele Report: Dmel\Irk2[UAS.cDa]

General Information

Symbol

Dmel\Irk2^UAS.cDa

Species

D. melanogaster

Name

FlyBase ID

FBal0277727

Feature type

allele

Associated gene

Dmel\Irk2

Associated Insertion(s)

Carried in Construct

P{UAS-Irk2.D}

Also Known As

UAS-irk2

Key Links

Transgenic product class

wild_type

(Dahal et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Dahal et al., 2012)

Mutagen

in vitro construct

(Dahal et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Irk2.D}

Cytology

Description

UASt regulatory sequences drive expression of wild type Irk2.

(Dahal et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Irk2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Dahal et al., 2012)

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Dahal et al., 2012)

wing vein, with Scer\GAL4^Bx-MS1096

(Dahal et al., 2012)

Detailed Description

Statement

Reference

Expression of Irk2^Scer\UAS.cDa under the control of Scer\GAL4^Bx-MS1096 results in minor vein defects in ~20% of wings, similar to Scer\GAL4^Bx-MS1096 in the absence of a UAS transgene. Very little apoptosis is seen in these flies.

No wing vein defects or blistering are seen when Irk2^Scer\UAS.cDa is expressed under the control of Scer\GAL4^en-e16E.

(Dahal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Irk2^UAS.cDa, Scer\GAL4^Bx-MS1096 has visible | adult stage phenotype, enhanceable by tkv⁷/tkv[+]

(Dahal et al., 2012)

NOT Enhanced by

Statement

Reference

Irk2^UAS.cDa, Scer\GAL4^da.G32 has visible | adult stage phenotype, non-enhanceable by dpp[+]/dpp^hr92

(Dahal et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Irk2^UAS.cDa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, non-enhanceable by dpp[+]/dpp^hr92

(Dahal et al., 2012)

Irk2^UAS.cDa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, non-enhanceable by tkv⁷/tkv[+]

(Dahal et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of dpp^hr92 does not enhance the wing vein defects seen when Irk2^Scer\UAS.cDa is expressed under the control of Scer\GAL4^Bx-MS1096.

One copy of tkv⁷ does not enhance the wing vein defects seen when Irk2^Scer\UAS.cDa is expressed under the control of Scer\GAL4^Bx-MS1096.

(Dahal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Act5C.PI/Irk2^UAS.cDa partially rescues Df(3R)irk2B/Df(3R)Exel6194

(Dahal et al., 2012)

Scer\GAL4^en-e16E/Irk2^UAS.cDa partially rescues Df(3R)irk2B/Df(3R)Exel6194

(Dahal et al., 2012)

Comments

Expression of Irk2^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PI partially rescues the wing defects seen in male Df(3R)Exel6194/Df(3R)irk2B mutant flies. All phenotypes except for the loss of the L5 vein are fully rescued, although the proportion of flies exhibiting this phenotype is reduced from 92% to 22%.

Expression of Irk2^Scer\UAS.cDa in the posterior compartment of the wing disc under the control of Scer\GAL4^en-e16E partially rescues the wing defects seen in Df(3R)Exel6194/Df(3R)irk2B mutant flies. All phenotypes except for the loss of the L5 vein are fully rescued, although the proportion of flies exhibiting this phenotype is reduced from 92% to 37%.

(Dahal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Irk2^Scer\UAS.cDa

Irk2^UAS.cDa

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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