FlyBase Allele Report: Dmel\aPKC[ts]

General Information

Symbol

Dmel\aPKC^ts

Species

D. melanogaster

Name

FlyBase ID

FBal0277054

Feature type

allele

Associated gene

Dmel\aPKC

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

temperature conditional hypomorphic allele

Mutagen

Nature of the Allele

Allele class

temperature conditional hypomorphic allele

(Guilgur et al., 2012)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: F532L.

(Guilgur et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:14,947,665..14,947,665 [-]

Nucleotide change:

T14947665C

Amino acid change:

Reported amino acid change:

F532L

Comment:

The nucleotide change was not reported and the position of the mutation is ambiguous. The TTT (Phe) codon may have changed to either CTT (Leu) or TTA/G (Leu). The annotated position is the first base of the codon.

(Guilgur et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | heat sensitive (with aPKC^k06403)

(Guilgur et al., 2012)

abnormal cytokinesis | heat sensitive (with Df(2R)l4)

(Guilgur et al., 2012)

abnormal planar polarity (with aPKC^k06403)

(Guilgur et al., 2012)

abnormal planar polarity (with Df(2R)l4)

(Guilgur et al., 2012)

abnormal size | heat sensitive (with aPKC^k06403)

(Guilgur et al., 2012)

abnormal size | heat sensitive (with Df(2R)l4)

(Guilgur et al., 2012)

increased cell death | first instar larval stage | somatic clone

(Guilgur et al., 2012)

increased cell death | third instar larval stage | heat sensitive (with aPKC^k06403)

(Guilgur et al., 2012)

increased cell death | third instar larval stage | heat sensitive (with Df(2R)l4)

(Guilgur et al., 2012)

lethal | heat sensitive (with aPKC^k06403)

(Guilgur et al., 2012)

lethal | heat sensitive (with Df(2R)l4)

(Guilgur et al., 2012)

sterile (with Df(2R)l4)

(Guilgur et al., 2012)

Phenotype Manifest In

centrosome | embryonic stage | maternal effect (with Df(2R)l4)

(Jiang et al., 2015)

embryo | dorsal closure stage | heat sensitive (with aPKC^k06403)

(Guilgur et al., 2012)

embryo | dorsal closure stage | heat sensitive (with Df(2R)l4)

(Guilgur et al., 2012)

female germline cyst (with aPKC^k06403)

(Guilgur et al., 2012)

female germline cyst (with Df(2R)l4)

(Guilgur et al., 2012)

female germline cyst | somatic clone

(Guilgur et al., 2012)

wing disc | third instar larval stage | heat sensitive (with aPKC^k06403)

(Guilgur et al., 2012)

wing disc | third instar larval stage | heat sensitive (with Df(2R)l4)

(Guilgur et al., 2012)

Detailed Description

Statement

Reference

Embryos from aPKC^ts/Df(2R)l4 mothers exhibit abnormal centrosome positioning in the embryonic epithelium.

(Jiang et al., 2015)

aPKC^ts/aPKC^k06403 flies are viable at the permissive temperature (25[o]C) and adult flies appear morphologically normal. Germline cyst encapsulation phenotypes are seen immediately after the mesenchymal-to-epithelial transition in region three of the germarium. The resulting follicular epithelium adherens junctions, apicobasal polarity and actin-myosin cytoskeleton appear normal. At the restrictive temperature (30[o]C) very few flies eclose from the pupal cases, with occasional escaper flies that exhibit abdominal dorsal closure defects. At the semi-permissive temperature (27-28[o]C) viability is variable, but almost all flies show significant dorsal closure defects.

aPKC^ts/Df(2R)l4 flies are viable at the permissive temperature (25[o]C) and adult flies are morphologically normal. However, females are sterile, with no larvae hatching from laid eggs even when crossed with wild type males. At the restrictive temperature (30[o]C) very few flies eclose from the pupal cases, with occasional escaper flies. At the semi-permissive temperature (27-28[o]C) viability is variable, but almost all flies show significant abdominal dorsal closure defects. Cyst encapsulation phenotypes are seen in aPKC^ts/Df(2R)l4 mutant ovaries at the permissive temperature.

aPKC^ts/aPKC^k06403 third instar larval wing discs show a temperature sensitive induction of apoptosis. Whereas at the permissive temperatures (18[o]C and 25[o]C) there are low levels of cell extrusion and apoptosis, at the restrictive temperature (30[0]C) significantly higher levels of cell extrusion and apoptosis are seen, and the wings discs appear smaller than in controls. No phenotypes are seen in the heterozygous controls.

aPKC^ts/Df(2R)l4 third instar larval wing discs show a temperature sensitive induction of apoptosis. Whereas at the permissive temperatures (18[o]C and 25[o]C) there are low levels of cell extrusion and apoptosis, at the restrictive temperature (30[0]C) significantly higher levels of cell extrusion and apoptosis are seen, and the wings discs appear smaller than in controls. Much of the apoptosis is found in the basal region of the disc. No phenotypes are seen in the heterozygous controls.

At both the permissive and restrictive temperatures there is a significant decrease in the proportion of aPKC^ts/aPKC^k06403 mutant wing disc cells with the correct planar spindle orientation compared to controls. The decrease is greater at 30[0]C than at 25[o]C.

At both the permissive and restrictive temperatures there is a significant decrease in the proportion of aPKC^ts/Df(2R)l4 mutant wing disc cells with the correct planar spindle orientation compared to controls. The decrease is greater at 30[0]C than at 25[o]C.

Large aPKC^ts wing disc clones induced in first instar larvae show temperature-sensitive apoptosis.

Embryos maternally mutant for aPKC^ts fail to complete germ band extension (GBE), with a complete loss of epithelial integrity at the permissive temperature. Embryos develop normally until the onset of gastrulation and germ band extension.

Embryos maternally mutant for aPKC^ts/aPKC^k06403 fail to complete germ band extension (GBE), with a complete loss of epithelial integrity at the permissive temperature.

Somatic follicular cell clones mutant for aPKC^ts show temperature-sensitive cyst encapsulation defects.

(Guilgur et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

aPKC^ts/aPKC^k06403 has increased cell death | heat sensitive phenotype, suppressible by raps[+]/pins¹⁹³

(Guilgur et al., 2012)

Other

Statement

Reference

aPKC^ts/Df(2R)l4, Scer\GAL4^{VP16.mat.αTub67C}, par-1^HMS00405 has increased cell size | maternal effect | embryonic stage phenotype

(Jiang et al., 2015)

BacA\p35^UAS.cHa, Df(2R)l4, Scer\GAL4^sd-SG29.1, aPKC^ts has neoplasia | larval stage phenotype

(Guilgur et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

aPKC^ts/Df(2R)l4 has centrosome | maternal effect | embryonic stage phenotype, suppressible by par-1^HMS00405/Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

aPKC^ts/aPKC^k06403 has wing disc | third instar larval stage | heat sensitive phenotype, suppressible by raps[+]/pins¹⁹³

(Guilgur et al., 2012)

aPKC^ts/aPKC^k06403 has embryo | dorsal closure stage | heat sensitive phenotype, suppressible by raps[+]/pins¹⁹³

(Guilgur et al., 2012)

Other

Statement

Reference

aPKC^ts/Df(2R)l4, Scer\GAL4^{VP16.mat.αTub67C}, par-1^HMS00405 has centrosome | increased number | maternal effect | embryonic stage phenotype

(Jiang et al., 2015)

BacA\p35^UAS.cHa, Df(2R)l4, Scer\GAL4^sd-SG29.1, aPKC^ts has wing disc | larval stage phenotype

(Guilgur et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Maternal expression of par-1^HMS00405 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the abnormal chromosome positioning of embryos from aPKC^ts/Df(2R)l4 mothers, but also results in enlarged cells with multiple centrosomes.

(Jiang et al., 2015)

raps¹⁹³ dominantly suppresses the temperature sensitive induction of apoptosis seen in aPKC^ts/aPKC^k06403 third instar larval wing discs at the semi-permissive temperature (28[o]C). However, this suppression does not occur at the restrictive temperature (30[o]C).

raps¹⁹³ dominantly suppresses the abdominal dorsal closure defects seen in aPKC^ts/aPKC^k06403 mutant flies at the semi-permissive temperature (28[o]C). However, this suppression did not occur at the restrictive temperature (30[o]C).

(Guilgur et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^sd-SG29.1 at the permissive temperature (25[o]C) induces the development of large tumour-like tissues in the basal region of aPKC^ts/Df(2R)l4 mutant larval wing discs. Expression of BacA\p35^Scer\UAS.cHa does not induce tumors in heterozygous controls.

(Guilgur et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

aPKC^ts

apkc-ts

(Guilgur et al., 2012)

apkc^ts

(Bergstralh et al., 2016, Jiang et al., 2015, Guilgur et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

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