FB2024_03 , released June 25, 2024
Allele: Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
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General Information
Symbol
Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
Species
a. artificial
Name
FlyBase ID
FBal0276804
Feature type
allele
Associated gene
Zzzz\CAG
Associated Insertion(s)
Carried in Construct
P{UAS-CAG.99}
Also Known As
rCAG~100
Transgenic product class
trinucleotide_repeat_expansion
(McLeod et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
trinucleotide_repeat_expansion
(McLeod et al., 2005)
Mutagen
in vitro construct
(McLeod et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-CAG.99}
Cytology
Description

UASt regulatory sequences drive expression of a Zzzz\CAG-repeat containing 99 poly-glutamines (above the human pathogenic threshold of about 40 repeats) fused with C-terminal Tag:MYC and Tag:FLAG tags.

(McLeod et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Zzzz\CAG
Tagged with
Tag:FLAG
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  neurodegenerative disease
      is ameliorated by CPV\CrPV-1AUASp.Tag:FLAG
      (Eyk et al., 2019)
      is ameliorated by Hsap\ADARUAS.p110.cHa
      (Eyk et al., 2019)
      is ameliorated by Hsap\ADARUAS.p150.cHa
      (Eyk et al., 2019)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (McLeod et al., 2005)
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Co-expression of Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in differentiated cells of the eye using Scer\GAL4GMR.PF causes disruption to ommatidial patterning, rough eye, and some loss of pigmentation.

      Glial co-expression of Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG with Scer\GAL4repo is highly toxic and leads to lethality in strong expression lines. Substantial lethality is observed in weaker expression lines as well. Compared with wild-type, mutant animals surviving to adulthood show age-dependent progressive deterioration of climbing ability.

      (Samaraweera et al., 2013)

      At the standard culture temperature of 25[o]C, no significant reduction in viability is observed for Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG-expressing lines using Scer\GAL4da.G32 compared with empty vector controls. When grown at 29[o]C, complete lethality is observed in Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG-expressing progeny but not in empty vector controls using Scer\GAL4da.G32.

      Closer physical examination of adult flies reveals a phenotype in viable progeny expressing Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG using Scer\GAL4da.G32 at 25[o]C whereby the tergites of the dorsal abdomen are not correctly formed. In wild-type adults, the abdomen contains a series of regularly arranged tergites, while in Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG-expressing flies one or more of the tergite bands is split down the midline so that the two sides do not meet at all, or meet only partially.

      Expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in histoblasts using Scer\GAL4T155 results in an insertion-line dependent mild tergite-disruption phenotype that shows variable expressivity.

      (Lawlor et al., 2012)

      All fly lines (except P{UAS-CAG.99}cherC) expressing Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG alone under the control of Scer\GAL4GMR.PF display normal eye patterning. Co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in a rough eye phenotype.

      Pan-neural co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG using Scer\GAL4elav-C155 results in a significant level of male lethality, while females show a mild phenotype in the adult eye.

      (Lawlor et al., 2011)

      Expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in the developing eye under the control of Scer\GAL4GMR.PF causes a dramatic phenotype with disorganization of the ommatidia, loss of pigment cells and a collapsed eye.

      Depending on the insertion line used, expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in phenotypes ranging in severity from wild-type to lethality.

      (McLeod et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by 18wHM05241, Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by MstProxHM05012, Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by Toll-4HM05029/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by Toll-7HM05230/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by TolloHM05005/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by Toll-9HM05227/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by spzHM05024/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by dlJF02825/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has visible phenotype, suppressible by Dcr-2[+]/Dcr-2L811fsX

      (Lawlor et al., 2011)
      NOT suppressed by
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by 18wHM05241, Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by MstProxHM05012, Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by Toll-4HM05029/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by Toll-7HM05230/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by TolloHM05005/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by Toll-9HM05227/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by spzHM05024/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has ommatidium phenotype, suppressible by dlJF02825/Scer\GAL4GMR.PF

      (Samaraweera et al., 2013)

      Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG has eye phenotype, suppressible by Dcr-2[+]/Dcr-2L811fsX

      (Lawlor et al., 2011)
      NOT suppressed by
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in the presence of heterozygous mblE27 using Scer\GAL4da.G32 leads to no significant change in tergite-phenotype proportion compared with expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG alone.

      Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background.

      One of two lines expressing Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG using Scer\GAL4da.G32 show a more severe tergite phenotype in the presence of heterozygous Dcr-1Q1147X than when Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG is expressed alone.

      (Lawlor et al., 2012)

      Heterozygous Dcr-2L811fsX suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2L811fsX as revealed by sections through adult eyes.

      Co-expression of Dcr-2Scer\UAS.cDa with Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG/Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG at 18[o]C results in a loss-of-pigment phenotype which is not observed when Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG/Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG is expressed under the control of Scer\GAL4GMR.PF.

      The normal eye phenotype of flies expressing Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF is not modified by the co-expression of Dcr-2Scer\UAS.cDa.

      The eye phenotype resulting from the co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG using Scer\GAL4elav-C155 is significantly enhanced when Dcr-2Scer\UAS.cDa is also co-expressed.

      Co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PLu fails to induce defects in the adult eye or viability, however, flies show an age-dependent movement disorder (failure-to-climb phenotype) compared with controls.

      (Lawlor et al., 2011)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG
      Zzzz\CAG99.T:Hsap\MYC,T:Zzzz\FLAG
      Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (7)