FB2024_03 , released June 25, 2024
Allele: Dmel\ana2719
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General Information
Symbol
Dmel\ana2719
Species
D. melanogaster
Name
FlyBase ID
FBal0269127
Feature type
allele
Associated gene
Dmel\ana2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Wang et al., 2011)
Mutagen
ethyl methanesulfonate
(Wang et al., 2011)
Progenitor genotype
Cytology
Description

Amino acid replacement: R175term.

(Wang et al., 2011)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:8,899,598..8,899,598 [-]
Nucleotide change:

C8899598T

Amino acid change:

R175term | ana2-PA; R175term | ana2-PB

Reported amino acid change:

R175term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wang et al., 2011)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ana2169/ana2719 adults are uncoordinated and fail to perform in climbing assay (negative gravitaxis assay) when compared to controls.

      (Aydogan et al., 2018)

      Homozygous larvae have an increased number of neuroblasts in the central brain at 96 hours after larval hatching (approximately 250 neuroblasts compared to approximately 100 neuroblasts in wild-type larvae at this stage).

      Homozygous clones in the larval brain result in overgrowth in both type I and type II neuroblast lineages. The number of intermediate neural progenitors (derived from type II neuroblasts in wild type) is also increased.

      Homozygous and ana2719/Df(2R)Np3 neuroblasts show severe defects in mitotic spindle orientation. The spindles are not focused at the poles during mitosis.

      75% of neuroblasts expressing ana2GD13865 under the control of Scer\GAL4insc-Mz1407 in a ana2719/+ background have a spindle misorientation phenotype.

      5% of homozygous larval brain clones transplanted into the abdomens of wild-type hosts form tumours, proliferating massively and killing the hosts rapidly.

      (Wang et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      ana2169/ana2719 is rescued by Scer\GAL4insc-Mz1407/ana2N1.UAS.Venus

      (Wang et al., 2011)
      Partially rescued by

      FBal0343975:, /ana2169/ana2719 is partially rescued by ana2UAS.ana2.EGFP/ana2UAS.ana2.EGFP

      (Aydogan et al., 2018)
      Not rescued by

      ana2169/ana2719 is not rescued by Scer\GAL4insc-Mz1407/ana2C1.UAS.Venus

      (Wang et al., 2011)

      ana2169/ana2719 is not rescued by Scer\GAL4insc-Mz1407/ana2N2.UAS.Venus

      (Wang et al., 2011)

      ana2169/ana2719 is not rescued by Scer\GAL4insc-Mz1407/ana2C2.UAS.Venus

      (Wang et al., 2011)
      Comments

      Expression of ana2N1.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4insc-Mz1407 almost fully rescues the centrosome assembly and spindle orientation defects of ana2169/ana2719 neuroblasts.

      Expression of either ana2C1.Scer\UAS.T:Avic\GFP-YFP.Venus, ana2N2.Scer\UAS.T:Avic\GFP-YFP.Venus or ana2C2.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4insc-Mz1407 fails to rescue the spindle orientation defects of ana2169/ana2719 neuroblasts.

      (Wang et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ana2719
      (Jo et al., 2019, Aydogan et al., 2018, Cottee et al., 2015, Wang et al., 2011)
      dSTIL719
      (Cottee et al., 2013)
      l(2)719
      (Wang et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)