Pvf1EPg11235, PvrUAS.GFP, Scer\GAL4slbo.2.6 has border follicle cell phenotype
The effect on border cell cluster migration of expressing PvrScer\UAS.T:Avic\GFP under the control of Scer\GAL4c522 in a single border follicle cell in a EgfrdsRNA.slbo Pvf1EP1624 background (to remove endogenous guidance information) has been studied. These clusters are dynamic and display disordered movement including cluster tumbling. In those border cell clusters where the single PvrScer\UAS.T:Avic\GFP-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the PvrScer\UAS.T:Avic\GFP-expressing cell is in the back, the cluster on average moves relatively more backwards and when the PvrScer\UAS.T:Avic\GFP-expressing cell is on the side of the cluster, border cell migration is unaltered compared to control clusters without a PvrScer\UAS.T:Avic\GFP-expressing cell.
Co-expression of Pvf1EPg11235 and PvrScer\UAS.T:Avic\GFP under the control of Scer\GAL4slbo.2.6 results in strong defects in border cell migration, with no migration being seen in approximately 60% of stage 10 egg chambers.