FlyBase Allele Report: Dmel\Pvf1[EPg11235]

General Information

Symbol

Dmel\Pvf1^EPg11235

Species

D. melanogaster

Name

FlyBase ID

FBal0127284

Feature type

allele

Associated gene

Dmel\Pvf1

Associated Insertion(s)

P{EPg}Pvf1^EPg11235

Carried in Construct

Also Known As

EP-Pvf1, EPg11235

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Duchek et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EPg}Pvf1^EPg11235

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPg}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type, with Scer\GAL4^dpp.blk1

(Macias et al., 2004)

wild-type, with Scer\GAL4^hh-Gal4

(Macias et al., 2004)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

border follicle cell & filopodium, with Scer\GAL4^slbo.2.6

(Fulga and Rorth, 2002)

border follicle cell | somatic clone, with Scer\GAL4^slbo.2.6

(Mathieu et al., 2007)

border follicle cell | somatic clone, with Scer\GAL4^Tub.PU

(Mathieu et al., 2007)

male terminalia, with Pvf1^UASp.cRb, Scer\GAL4^en-e16E

(Macias et al., 2004)

male terminalia, with Pvf1^UASp.cRb, Scer\GAL4^hh-Gal4

(Macias et al., 2004)

male terminalia, with Scer\GAL4^en-e16E

(Macias et al., 2004)

male terminalia, with Scer\GAL4^hh-Gal4

(Macias et al., 2004)

Detailed Description

Statement

Reference

Border follicle cell clones expressing Pvf1^EPg11235 under the control of Scer\GAL4^tub show mild defects in posterior migration towards the oocyte.

Border follicle cells expressing Pvf1^EPg11235 under the control of Scer\GAL4^slbo.2.6 show mild defects in posterior migration towards the oocyte.

(Mathieu et al., 2007)

When expression is driven by Scer\GAL4^dpp.blk1 or Scer\GAL4^ptc-559.1 there is no detectable phenotype. Expression driven by Scer\GAL4^en-e16E or Scer\GAL4^hh-Gal4 causes incompletely penetrant male terminalia rotation phenotype. Penetrance is increased by the addition of extra Pvf1, in the form of P{UASp2-Pvf1.Rb}.

(Macias et al., 2004)

Border cells in the ovaries of Pvf1^EPg11235; Scer\GAL4^slbo.2.6 animals form significantly fewer long cellular extensions (20-40 μm) and intermediate cellular extensions (10-20 μm).

(Fulga and Rorth, 2002)

When Pvf1^EPg11235 is driven by Scer\GAL4^slbo.2.6, a modest but detectable delay on border cell migration is seen: All border cell clusters move by stage 10, and one third arrive at the oocyte.

(Duchek et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Pvf1^EPg11235, Scer\GAL4^slbo.2.6 has border follicle cell | somatic clone phenotype, enhanceable by hep^Act.UAS, Scer\GAL4^slbo.2.6

(Mathieu et al., 2007)

Pvf1^EPg11235, Scer\GAL4^slbo.2.6 has border follicle cell & filopodium phenotype, enhanceable by Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(Fulga and Rorth, 2002)

Pvf1^EPg11235, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Pvr^UAS.cDa, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

NOT Enhanced by

Statement

Reference

Pvf1^EPg11235, Scer\GAL4^Tub.PU has border follicle cell | somatic clone phenotype, non-enhanceable by puc^PBac3929/puc^PBac3929

(Mathieu et al., 2007)

Pvf1^EPg11235, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, non-enhanceable by vn^EPg35521, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

NOT suppressed by

Statement

Reference

Pvf1^EPg11235, Scer\GAL4^Tub.PU has border follicle cell | somatic clone phenotype, non-suppressible by puc^PBac3929/puc^PBac3929

(Mathieu et al., 2007)

Enhancer of

Statement

Reference

Pvf1^EPg11235, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(Fulga and Rorth, 2002)

Other

Statement

Reference

Pvf1^EPg11235, Pvr^UAS.GFP, Scer\GAL4^slbo.2.6 has border follicle cell phenotype

(Inaki et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Pvf1^EPg11235 and Pvr^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^slbo.2.6 results in strong defects in border cell migration, with no migration being seen in approximately 60% of stage 10 egg chambers.

(Inaki et al., 2012)

The posterior migration defects seen in border follicle cell clones expressing Pvf1^EPg11235 under the control of Scer\GAL4^tub are not affected if the cells are also homozygous for puc^PBac3929.

The posterior migration defects seen in border follicle cells expressing Pvf1^EPg11235 under the control of Scer\GAL4^slbo.2.6 are slightly enhanced if the cells are also co-expressing hep^Act.Scer\UAS.

(Mathieu et al., 2007)

Border cells in the ovaries of Pvf1^EPg11235; Pvr^DN.Scer\UAS; Scer\GAL4^slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm).

(Fulga and Rorth, 2002)

When Pvr^Scer\UAS.cDa is added to Pvf1^EPg11235, Scer\GAL4^slbo.2.6 an enhancement of the border cell migration phenotype is seen. A quarter of the border cells at stage 10 are at the oocyte, but another quarter do not move at all. The addition of vn^EPg35521 has no effect. the addition of Pvf1^EPg11235 to Egfr^DN.Scer\UAS when driven by Scer\GAL4^slbo.2.6 leads to an enhancement of the border cell migration phenotype. Border cells do not reach the oocyte at stage 10. They usually migrate less than halfway, and are sometimes found off track.

(Duchek et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Pvf1^EPg11235

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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