Scer\GAL80FRT.αTub84B is a non-suppressor of visible phenotype of DCTN1-p150Δ.UAS, Scer\GAL4CCAP.PP
Scer\GAL80FRT.αTub84B is a non-suppressor of visible phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF
Scer\GAL80FRT.αTub84B is a non-suppressor of wing phenotype of DCTN1-p150Δ.UAS, Scer\GAL4CCAP.PP
Scer\GAL80FRT.αTub84B is a non-suppressor of eye phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF
Scer\GAL80Scer\FRT.αTub84B has no effect on the wing expansion defect caused by expression of GlΔ.Scer\UAS under the control of Scer\GAL4Ccap.PP. When FLPase is used to remove the Scer\FRT cassette in Scer\GAL80Scer\FRT.αTub84B, generating Scer\GAL80αTub84B.PB which expresses Scer\GAL80, the failure of wing expansion caused by expression of GlΔ.Scer\UAS under the control of Scer\GAL4Ccap.PP is completely suppressed.
Scer\GAL80Scer\FRT.αTub84B has no effect on the eye degeneration and depigmentation caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF. When FLPase is used to remove the Scer\FRT cassette in Scer\GAL80Scer\FRT.αTub84B, generating Scer\GAL80αTub84B.PB which expresses Scer\GAL80, the eye phenotype caused by expression of Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is completely suppressed.
Flies carrying Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF and Scer\GAL80αTub84B.PG have normal eyes, due to repression of Scer\GAL4GMR.PF expression by Scer\GAL80αTub84B.PG. This repression can be removed using FLPase (which removes the Scer\FRT cassette containing the Scer\GAL80 coding region from Scer\GAL80αTub84B.PG).