UAS regulatory sequences drive expression of a short inverted repeat.
Adults expressing parkHMS01651 under the control of Scer\GAL4Act5C.PI or Scer\GAL4ple.PF show a progressive decline in climbing activity, and a progressive decrease in DA neuron number (PPL1, PPL2, PPM1/2 and PPM3 clusters); DA neurons also show decreased abundance of mitochondria.
The expression of parkHMS01651 under the control of Scer\GAL4Act.PU in clones within mosaic midguts results in enterocytes during pupariation, but not after puparium formation, exhibiting a significant increase in the transverse cell area, a significant increase in the relative mitochondrial content and a significant decrease in the relative amount of autophagosomes, as assessed by a Atg8a fluorescent reporter, as compared to non-clone enterocytes.
Expression of parkHMS01651 under the control of Scer\GAL4tin.CΔ4 evokes heart tube hypocontractility at one and four weeks after eclosion, and increases cardiomyocyte mitochondrial size. The lifespan of these flies is similar to controls but the flies display an accelerated age-related decline in climbing performance. Heart tube end-systolic and end-diastolic dimensions are increased and mitochondria have decreased DNA, increase mitochondrial reactive oxygen species (ROS) and increased numbers of depolarized organelles.
Scer\GAL4ple.PF, parkHMS01651 has decreased cell number | adult stage | progressive phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has abnormal neuroanatomy | adult stage phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has abnormal neuroanatomy | adult stage phenotype, suppressible by ParisGD9020, Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has decreased cell number | adult stage | progressive phenotype, suppressible by ParisGD9020, Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by ParisGD9020, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is an enhancer of decreased cell number | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is an enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is a non-enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is a non-enhancer of decreased cell number | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is a non-enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4GMR.long is a suppressor of visible phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.long
parkHMS01651, Scer\GAL4ple.PF is a non-suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is a non-suppressor of decreased cell number | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is a non-suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has dopaminergic neuron | adult stage | decreased number phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has mitochondrion | adult stage phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF
Scer\GAL4ple.PF, parkHMS01651 has dopaminergic neuron | adult stage | decreased number phenotype, suppressible by ParisGD9020, Scer\GAL4ple.PF
Scer\GAL4tin.CΔ4, parkHMS01651 has mitochondrion phenotype, suppressible by Sod1UAS.cWa/Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has mitochondrion phenotype, suppressible by Sod2UAS.cMa/Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has mitochondrion phenotype, suppressible by MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has adult heart phenotype, suppressible by Sod1UAS.cWa/Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has adult heart phenotype, suppressible by Sod2UAS.cMa/Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has adult heart phenotype, suppressible by MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has cardial cell phenotype, suppressible by Sod1UAS.cWa/Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has cardial cell phenotype, suppressible by Sod2UAS.cMa/Scer\GAL4tin.CΔ4
Scer\GAL4tin.CΔ4, parkHMS01651 has cardial cell phenotype, suppressible by MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
parkHMS01651, Scer\GAL4ple.PF is an enhancer of dopaminergic neuron | adult stage | decreased number phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is an enhancer of mitochondrion | adult stage phenotype of Hsap\ZNF746UAS.cDa, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4ple.PF is a non-enhancer of dopaminergic neuron | adult stage | decreased number phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
parkHMS01651, Scer\GAL4GMR.long is a suppressor of eye phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.long
parkHMS01651, Scer\GAL4tin.CΔ4 is a suppressor of adult heart phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
parkHMS01651, Scer\GAL4tin.CΔ4 is a suppressor of cardial cell phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
parkHMS01651, Scer\GAL4tin.CΔ4 is a suppressor | partially of mitochondrion phenotype of MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4
parkHMS01651, Scer\GAL4ple.PF is a non-suppressor of dopaminergic neuron | adult stage | decreased number phenotype of ParisUAS.ORF.GW.Tag:HA, Scer\GAL4ple.PF
Expression of SodScer\UAS.cWa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.
Expression of Sod2Scer\UAS.cMa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.
Expression of CG6878KK108566 suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.
Co-expression of MarfmiRNA.cUa.Scer\UAS and parkHMS01651 under the control of Scer\GAL4tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content usually seen in flies expressing parkHMS01651 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen in parkHMS01651, and appear fragmented as is seen with MarfmiRNA.cUa.Scer\UAS.
Co-expression of parkHMS01651 partially suppresses eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Co-expression of parkHMS01651 does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies.