FlyBase Allele Report: Dmel\ckn[K.Δ324-331]

General Information

Symbol

Dmel\ckn^K.Δ324-331

Species

D. melanogaster

Name

FlyBase ID

FBal0265796

Feature type

allele

Associated gene

Dmel\ckn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Weng et al., 2011)

Mutagen

ethyl methanesulfonate

(Weng et al., 2011)

Progenitor genotype

P{GSV1}GS1205

(Weng et al., 2011)

Cytology

Description

Deletion which removes ckn residues 324-331, but does not result in a frameshift.

(Weng et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Weng et al., 2011)

abnormal neuroanatomy | embryonic stage (with Df(2R)BSC330)

(Weng et al., 2011)

Phenotype Manifest In

larval intersegmental nerve | embryonic stage

(Weng et al., 2011)

larval intersegmental nerve | embryonic stage (with Df(2R)BSC330)

(Weng et al., 2011)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Weng et al., 2011)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage (with Df(2R)BSC330)

(Weng et al., 2011)

Detailed Description

Statement

Reference

ckn^K.Δ324-331 homozygous or ckn^K.Δ324-331/Df(2R)BSC330 mutant embryos display consistent motor axon projection defects, exhibiting classic ISNb 'bypass' phenotypes, where ISNb axons fail to innervate the ventrolateral muscle field. These axons appear to defasciculate normally from the primary ISN branch at the exit junction, but fail to enter the ventral muscle field, instead bypassing their targets as they extend parallel to the primary ISN fascicle. Frequently, mis-targeted ISNb axons reach back from the dorsal edge of muscle 12 to innervate the ventrolateral muscle field. In the majority of affected nerves, ISNb axons are visible as a distinct fascicle next to the ISN.

ckn^K.Δ324-331 heterozygous embryos do not exhibit ISNb guidance defects.

(Weng et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

ckn^K.Δ324-331 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Ptp69D¹/Ptp69D[+]

(Weng et al., 2011)

ckn^K.Δ324-331 has abnormal neuroanatomy | embryonic stage phenotype, non-enhanceable by Ptp69D[+]/Ptp69D⁷

(Weng et al., 2011)

NOT suppressed by

Statement

Reference

ckn^K.Δ324-331 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Ptp69D¹/Ptp69D[+]

(Weng et al., 2011)

ckn^K.Δ324-331 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Ptp69D[+]/Ptp69D⁷

(Weng et al., 2011)

Enhancer of

Statement

Reference

ckn^K.Δ324-331 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Lar^5.5

(Weng et al., 2011)

Other

Statement

Reference

ckn^K.Δ324-331, dock³ has abnormal neuroanatomy | embryonic stage phenotype

(Weng et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

ckn^K.Δ324-331 has larval intersegmental nerve | embryonic stage phenotype, non-enhanceable by Ptp69D¹/Ptp69D[+]

(Weng et al., 2011)

ckn^K.Δ324-331 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-enhanceable by Ptp69D¹/Ptp69D[+]

(Weng et al., 2011)

ckn^K.Δ324-331 has larval intersegmental nerve | embryonic stage phenotype, non-enhanceable by Ptp69D[+]/Ptp69D⁷

(Weng et al., 2011)

ckn^K.Δ324-331 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-enhanceable by Ptp69D[+]/Ptp69D⁷

(Weng et al., 2011)

NOT suppressed by

Statement

Reference

ckn^K.Δ324-331 has larval intersegmental nerve | embryonic stage phenotype, non-suppressible by Ptp69D¹/Ptp69D[+]

(Weng et al., 2011)

ckn^K.Δ324-331 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-suppressible by Ptp69D¹/Ptp69D[+]

(Weng et al., 2011)

ckn^K.Δ324-331 has larval intersegmental nerve | embryonic stage phenotype, non-suppressible by Ptp69D[+]/Ptp69D⁷

(Weng et al., 2011)

ckn^K.Δ324-331 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-suppressible by Ptp69D[+]/Ptp69D⁷

(Weng et al., 2011)

Enhancer of

Statement

Reference

ckn^K.Δ324-331 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of dock³

(Weng et al., 2011)

ckn^K.Δ324-331 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Lar^5.5

(Weng et al., 2011)

ckn^K.Δ324-331/ckn[+] is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of dock³

(Weng et al., 2011)

Other

Statement

Reference

ckn^K.Δ324-331, dock³ has larval intersegmental nerve branch ISNd of A1-7 phenotype

(Weng et al., 2011)

ckn^K.Δ324-331, dock³ has lateral longitudinal fascicle | embryonic stage phenotype

(Weng et al., 2011)

ckn^K.Δ324-331, dock³ has intermediate longitudinal fascicle | embryonic stage phenotype

(Weng et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Lar^5.5 ckn^K.Δ324-331 double heterozygous embryos display bypass phenotypes with 39% penetrance.

A heterozygous or homozygous Ptp69D¹ background fails to affect the penetrance of the ISNb bypass phenotype found in ckn^K.Δ324-331 heterozygotes or homozygotes.

A heterozygous or homozygous Ptp69D⁷ background fails to affect the penetrance of the ISNb bypass phenotype found in ckn^K.Δ324-331 heterozygotes or homozygotes.

ISNb pathfinding is delayed in approximately 23% of dock³ ckn^K.Δ324-331 double heterozygotes.

A heterozygous ckn^K.Δ324-331 background enhances ISNb pathfinding defects in dock³ homozygotes, from 6% to 43% of hemisegments.

dock³ ckn^K.Δ324-331 double homozygotes exhibit delayed 'immature' ISNb axons in 65% of hemisegments. Motor axons in the affected nerves are loosely organised with multiple projections and resemble wild-type axons at earlier stages. Furthermore, the ISNd branch is frequently absent or reduced in size. Examination of the ISNb/d choice point reveals defects in ISNb/d branch segregation. The lateral two longitudinal Fas2-positive fascicles are poorly fasciculated and discontinuous in these double mutants.

(Weng et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ckn^K.Δ324-331/Scer\GAL4^elav.PLu rescues ckn^UAS.cWa

(Weng et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ckn^K.Δ324-331

ckn^K

(Weng et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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