FlyBase Allele Report: Dmel\w[RNAi.GMR]

General Information

Symbol

Dmel\w^RNAi.GMR

Species

D. melanogaster

Name

FlyBase ID

FBal0265706

Feature type

allele

Associated gene

Dmel\w

Associated Insertion(s)

Carried in Construct

P{GMR-w.RNAi}

Also Known As

GMR-wIR, GMR-w^IR

Key Links

Transgenic product class

RNAi_reagent

(Lee et al., 2004, Lee and Carthew, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lee et al., 2004, Lee and Carthew, 2003)

Mutagen

in vitro construct

(Lee et al., 2004, Lee and Carthew, 2003)

Progenitor genotype

Carried in construct

P{GMR-w.RNAi}

Cytology

Description

Consists of two copies of the w exon 3 in opposite orientation to each other and separated by a w intron. The construct is terminated with a Hsp70 terminator. The resulting transcript after intron splicing, is predicted to create a perfect hairpin dsRNA of length 629 bp.

(Lee et al., 2004)

The GMR promoter drives expression of a tail-to-tail w repeat. The repeat comprises the 629bp third exon of w ligated to the 74bp second intron of w, ligated to another copy of the w third exon in the opposite orientation to the first copy.

(Lee and Carthew, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color

(Lim et al., 2014, Lee et al., 2009, Lim et al., 2008, Zhou et al., 2008, Pelisson et al., 2007, Kupsco et al., 2006, van Rij et al., 2006, Forstemann et al., 2005, Lee and Carthew, 2003)

abnormal eye color | semidominant

(Lee et al., 2020)

visible

(Fukunaga et al., 2012, Berry et al., 2009, Lee and Carthew, 2003)

Phenotype Manifest In

eye

(Lim et al., 2014, Fukunaga et al., 2012, Marques et al., 2010, Berry et al., 2009, Lee et al., 2009, Lim et al., 2008, Pelisson et al., 2007, Kupsco et al., 2006, Lee et al., 2004)

pigment cell

(Zhou et al., 2008, van Rij et al., 2006, Forstemann et al., 2005, Lee et al., 2004, Lee and Carthew, 2003)

Detailed Description

Statement

Reference

Expression of w^dsRNA.GMR/+ leads to pale orange eye color while expression of w^dsRNA.GMR/w^dsRNA.GMR leads to almost white eye color.

(Lee et al., 2020)

Two copies of the P{GMR-w.IR} transgene leads to an almost complete loss of eye pigmentation, while a single copy results in a less stringent eye phenotype, with a light yellow or orange eye color.

(Lim et al., 2014)

w^dsRNA.GMR produces white eyes in a wild-type genetic background.

(Fukunaga et al., 2012)

Flies carrying w^dsRNA.GMR show a 90-fold reduction in drosopterin pigment levels compared to wild type.

(Harris et al., 2011)

Flies expressing two copies of w^IR.GMR exhibit white eyes due to complete silencing of the w gene.

(Marques et al., 2010)

Expression of w^dsRNA.GMR suppresses eye pigmentation.

(Berry et al., 2009)

The adult eyes of w^IR.GMR flies are lightened from bright red to light orange.

(Lee et al., 2009)

Flies carrying one copy of the w^IR.GMR transgene have a pale orange eye colour.

(Lim et al., 2008)

Flies carrying w^IR.GMR have a pale eye colour.

(Zhou et al., 2008)

w^IR.GMR heterozygous flies exhibit a pale orange eye colour.

(Kim et al., 2007)

Flies carrying one copy of w^dsRNA.GMR in a wild-type background have yellowish eyes.

(Pelisson et al., 2007)

Flies carrying w^dsRNA.GMR have faint orange eyes.

(Kupsco et al., 2006)

Eye colour: flies expressing w^IR.GMR have orange eyes.

(van Rij et al., 2006)

Eye colour: flies carrying P{GMR-wIR} show silencing of the w gene, which is proportionate to the number of copies of P{GMR-wIR}, resulting in orange eyes in flies carrying one copy of P{GMR-wIR} and white eyes in flies carrying two copies of P{GMR-wIR}.

(Forstemann et al., 2005)

One copy of P{GMR-wIR} produces pale orange eyes, 2 copies white eyes.

(Lee et al., 2004)

Adult females transfected with one copy of w^dsRNA.GMR have yellow or pale yellow eyes, depending on the insertion. All flies from each line exhibit a uniform eye colour phenotype, indicating strong penetrance and expressivity of the RNAi effect. Adults transfected with two copies of w^dsRNA.GMR have white eyes, like flies that carry null w alleles.

(Lee and Carthew, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

w^RNAi.GMR has abnormal eye color phenotype, enhanceable by HPS4^Q119X

(Lee et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, enhanceable by HPS4^E133X

(Lee et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, enhanceable by HPS4^Q259X

(Lee et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, enhanceable by HPS4^W515X

(Lee et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, enhanceable by HPS4^Q664X

(Lee et al., 2009)

Dcr-1^Q1147X, w^RNAi.GMR has abnormal size phenotype, enhanceable by FBXO11^Q803R

(Lee et al., 2009)

NOT Enhanced by

Statement

Reference

w^RNAi.GMR has abnormal eye color | semidominant phenotype, non-enhanceable by Hsap\CMTR1^UAS.cLa/Scer\GAL4^GMR.PFa, Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color | semidominant phenotype, non-enhanceable by Cmtr1^UAS.cLa/Scer\GAL4^GMR.PFa, Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel6058/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel6062/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel9015/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel7138/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel6077/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by +/Df(2R)Exel7173

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3L)Exel9000/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3L)Exel7210/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel9014/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2L)Exel6027/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by +/Df(1)Exel6221

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2L)Exel6036/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by +/Df(2R)Exel7094

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel6056/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel7162/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel6072/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Exel7171/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by +/Df(3L)Exel6279

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3L)Exel6123/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3L)Exel6132/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3L)Exel6135/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(1)Exel6223/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6145/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6148/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6153/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6154/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6265/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6157/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6162/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6170/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6275/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6180/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(1)Exel6255/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel6199/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(3R)Exel8178/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by E(Pc)[+]/E(Pc)^w3

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Psc[+]/Psc¹

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Psc[+]/Psc^e23

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Psc^e25/Psc[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Psc^h28/Psc[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2R)Su(z)2-1.b8/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2^k06344/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2^4d1/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2L)Exel7002/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2^s15/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2[+]/Su(z)2^s20

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2^s95/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Su(z)2^sM/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2L)Exel8003/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2L)Exel6002/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by +/Df(2L)Exel6006

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Df(2L)Exel6049/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-enhanceable by Snp^c00465/Snp^c00465

(Kupsco et al., 2006)

Suppressed by

Statement

Reference

w^RNAi.GMR has abnormal eye color | semidominant phenotype, suppressible by Hsap\CMTR1^K239A.UAS/Scer\GAL4^GMR.PFa, Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color | semidominant phenotype, suppressible by Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color | semidominant phenotype, suppressible by AGO2⁴¹⁴/AGO2⁴¹⁴

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color | semidominant phenotype, suppressible by Cmtr1^K179A.UAS/Scer\GAL4^GMR.PFa, Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Df(3L)ED4978/+

(Lim et al., 2014)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by CRIF^EY03252/CG7172[+]

(Lim et al., 2014)

w^RNAi.GMR has visible phenotype, suppressible by loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, suppressible by loqs^PB.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, suppressible by loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, suppressible by Scer\GAL4^GMR.PF/FHV\B2^{UASp.Tag:FLAG}

(Berry et al., 2009)

w^RNAi.GMR has visible phenotype, suppressible by DCV\1A^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has visible phenotype, suppressible by TBSV\P19^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has visible phenotype, suppressible by PCV\P15^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has visible phenotype, suppressible by BYV\P21^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by FBXO11^Q803R

(Lee et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-1^Q1147X

(Lee et al., 2009)

Dcr-1^Q1147X, w^RNAi.GMR has abnormal eye color phenotype, suppressible by FBXO11^Q803R

(Lee et al., 2009)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^P8S

(Lim et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^A1453T

(Lim et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^R269W

(Lim et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^P365L

(Lim et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^P1496L

(Lim et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^L188F

(Lim et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by bel⁶

(Zhou et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^L811fsX/Dcr-2^L811fsX

(Pelisson et al., 2007)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by Dcr-2^unspecified/Dcr-2^unspecified

(Kupsco et al., 2006)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by DCV\1A^UASp.cvRa/Scer\GAL4^GMR.PF

(van Rij et al., 2006)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by loqs^f00791/loqs^f00791

(Forstemann et al., 2005)

w^RNAi.GMR has abnormal eye color phenotype, suppressible by r2d2^unspecified/r2d2^unspecified

(Forstemann et al., 2005)

NOT suppressed by

Statement

Reference

w^RNAi.GMR has abnormal eye color | semidominant phenotype, non-suppressible by Hsap\CMTR1^UAS.cLa/Scer\GAL4^GMR.PFa, Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has abnormal eye color | semidominant phenotype, non-suppressible by Cmtr1^UAS.cLa/Scer\GAL4^GMR.PFa, Cmtr1^W231X/Cmtr1^W231X

(Lee et al., 2020)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs^PB.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has visible phenotype, non-suppressible by loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by +/Df(1)Exel6221

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(1)Exel6223/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(1)Exel6255/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2L)Exel7002/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2L)Exel8003/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2L)Exel6002/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by +/Df(2L)Exel6006

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2L)Exel6049/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel6058/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel6062/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel9015/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel7138/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel6077/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by +/Df(2R)Exel7173

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3L)Exel9000/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3L)Exel7210/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel9014/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2L)Exel6027/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2L)Exel6036/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by +/Df(2R)Exel7094

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel6056/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel7162/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel6072/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Exel7171/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by +/Df(3L)Exel6279

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3L)Exel6123/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3L)Exel6132/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3L)Exel6135/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6145/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6148/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6153/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6154/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6265/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6157/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6162/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6170/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6275/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6180/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel6199/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(3R)Exel8178/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by E(Pc)[+]/E(Pc)^w3

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Psc[+]/Psc¹

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Psc[+]/Psc^e23

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Psc^e25/Psc[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Psc^h28/Psc[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Df(2R)Su(z)2-1.b8/+

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2^k06344/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2^4d1/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2^s15/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2[+]/Su(z)2^s20

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2^s95/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Su(z)2^sM/Su(z)2[+]

(Ji et al., 2012)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Hsc70-4^54.1

(Zhou et al., 2008)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by aub^HN2/aub^N11

(Pelisson et al., 2007)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by piwi¹

(Pelisson et al., 2007)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by piwi⁰⁶⁸⁴³

(Pelisson et al., 2007)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by Scer\GAL4^GMR.PF/DCV\1A^KK73AA.UASp

(van Rij et al., 2006)

w^RNAi.GMR has abnormal eye color phenotype, non-suppressible by r2d2[+]/r2d2^unspecified

(Forstemann et al., 2005)

Other

Statement

Reference

Dcr-1^Q1147X, w^RNAi.GMR has abnormal size phenotype

(Lee et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

w^RNAi.GMR has eye phenotype, enhanceable by HPS4^Q119X

(Lee et al., 2009)

w^RNAi.GMR has eye phenotype, enhanceable by HPS4^E133X

(Lee et al., 2009)

w^RNAi.GMR has eye phenotype, enhanceable by HPS4^Q259X

(Lee et al., 2009)

w^RNAi.GMR has eye phenotype, enhanceable by HPS4^W515X

(Lee et al., 2009)

w^RNAi.GMR has eye phenotype, enhanceable by HPS4^Q664X

(Lee et al., 2009)

Dcr-1^Q1147X, w^RNAi.GMR has eye phenotype, enhanceable by FBXO11^Q803R

(Lee et al., 2009)

w^RNAi.GMR has eye phenotype, enhanceable by E(GMR-wIR)A28^A28

(Lee et al., 2004)

w^RNAi.GMR has pigment cell phenotype, enhanceable by E(GMR-wIR)A28^A28

(Lee et al., 2004)

NOT Enhanced by

Statement

Reference

w^RNAi.GMR has eye phenotype, non-enhanceable by Snp^c00465/Snp^c00465

(Kupsco et al., 2006)

Suppressed by

Statement

Reference

w^RNAi.GMR has eye phenotype, suppressible by loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, suppressible by loqs^PB.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, suppressible by loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^L812bsX

(Marques et al., 2010)

w^RNAi.GMR has eye phenotype, suppressible by loqs^KO

(Marques et al., 2010)

w^RNAi.GMR has eye phenotype, suppressible by loqs^f00791

(Marques et al., 2010)

w^RNAi.GMR has eye phenotype, suppressible by r2d2^S165fsX

(Marques et al., 2010)

w^RNAi.GMR has eye phenotype, suppressible by r2d2^S165fsX/loqs^KO

(Marques et al., 2010)

w^RNAi.GMR has eye phenotype, suppressible by loqs^f00791/r2d2¹

(Marques et al., 2010)

w^RNAi.GMR has eye phenotype, suppressible by Scer\GAL4^GMR.PF/FHV\B2^{UASp.Tag:FLAG}

(Berry et al., 2009)

w^RNAi.GMR has eye phenotype, suppressible by DCV\1A^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has eye phenotype, suppressible by TBSV\P19^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has eye phenotype, suppressible by PCV\P15^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has eye phenotype, suppressible by BYV\P21^{UASp.Tag:FLAG}/Scer\GAL4^GMR.PF

(Berry et al., 2009)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-1^Q1147X

(Lee et al., 2009, Lee et al., 2004)

w^RNAi.GMR has eye phenotype, suppressible by FBXO11^Q803R

(Lee et al., 2009)

Dcr-1^Q1147X, w^RNAi.GMR has eye phenotype, suppressible by FBXO11^Q803R

(Lee et al., 2009)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^L811fsX/Dcr-2^L811fsX

(Pelisson et al., 2007)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^unspecified/Dcr-2^unspecified

(Kupsco et al., 2006)

w^RNAi.GMR has pigment cell phenotype, suppressible by DCV\1A^UASp.cvRa/Scer\GAL4^GMR.PF

(van Rij et al., 2006)

w^RNAi.GMR has pigment cell phenotype, suppressible by r2d2^unspecified/r2d2^unspecified

(Forstemann et al., 2005)

w^RNAi.GMR has pigment cell phenotype, suppressible by loqs^f00791/loqs^f00791

(Forstemann et al., 2005)

w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^C473Y

(Lee et al., 2004)

w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^G173E

(Lee et al., 2004)

w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^R416X

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^E1210V.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^E1371K.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^E1617K.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^E1210V.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^E1371K.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^E1617K.t7.2

(Lee et al., 2004)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^A500V

(Lee et al., 2004)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^C473Y

(Lee et al., 2004)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^G173E

(Lee et al., 2004)

w^RNAi.GMR has eye phenotype, suppressible by Dcr-2^R416X

(Lee et al., 2004)

w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-1^Q1147X

(Lee et al., 2004)

w^RNAi.GMR has pigment cell phenotype, suppressible by Dcr-2^A500V

(Lee et al., 2004)

NOT suppressed by

Statement

Reference

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs^PB.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs[+]/loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by loqs^PB.Tag:FLAG/loqs^PA.Tag:FLAG/loqs^KO/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

w^RNAi.GMR has eye phenotype, non-suppressible by piwi¹/piwi¹

(Pelisson et al., 2007)

w^RNAi.GMR has eye phenotype, non-suppressible by piwi⁰⁶⁸⁴³/piwi⁰⁶⁸⁴³

(Pelisson et al., 2007)

w^RNAi.GMR has eye phenotype, non-suppressible by aub^HN2/aub^N11

(Pelisson et al., 2007)

w^RNAi.GMR has pigment cell phenotype, non-suppressible by Scer\GAL4^GMR.PF/DCV\1A^KK73AA.UASp

(van Rij et al., 2006)

w^RNAi.GMR has pigment cell phenotype, non-suppressible by r2d2[+]/r2d2^unspecified

(Forstemann et al., 2005)

Dcr-2^L811fsX, w^RNAi.GMR has eye phenotype, non-suppressible by Dcr-2^{E1371K.E1617K.t7.2}

(Lee et al., 2004)

Dcr-2^L811fsX, w^RNAi.GMR has pigment cell phenotype, non-suppressible by Dcr-2^{E1371K.E1617K.t7.2}

(Lee et al., 2004)

Other

Statement

Reference

Dcr-1^Q1147X, w^RNAi.GMR has eye phenotype

(Lee et al., 2009, Lee et al., 2004)

Dcr-1^Q1147X, w^RNAi.GMR has ommatidium phenotype

(Lee et al., 2004)

Dcr-1^Q1147X, w^RNAi.GMR has interommatidial bristle phenotype

(Lee et al., 2004)

Dcr-1^Q1147X, w^RNAi.GMR has microchaeta phenotype

(Lee et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The eyes of w^dsRNA.GMR transgenic female flies homozygous for any of AGO2⁴¹⁴, Dcr-2^L811fsX, Dcr-2^G31R or r2d2¹ are similar to that of wild-type.

(Fukunaga et al., 2012)

The reduction in drosopterin pigment levels seen in flies carrying w^dsRNA.GMR is enhanced manyfold if they are also mutant for one of g¹, HPS4^W515X, p¹ or ca¹.

The reduction in drosopterin pigment levels seen in flies carrying w^dsRNA.GMR is not enhanced by car¹.

(Harris et al., 2011)

Dcr-2^L812bsX mutant flies carrying two copies of w^IR.GMR fail to silence the w gene and exhibit red eyes.

AGO2⁴¹⁴ mutant flies carrying two copies of w^IR.GMR fail to silence the w gene and exhibit red eyes.

loqs^KO mutant flies carrying two copies of w^IR.GMR exhibit impaired w silening and display orange-red eyes.

loqs^f00791 mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

r2d2¹ mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

r2d2^S165fsX mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^f00791 r2d2¹ double mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^KO r2d2^S165fsX double mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

(Marques et al., 2010)

The lighter colour of w^IR.GMR eyes is enhanced when eyes are also made homozygous mutant for HPS4^Q119X, HPS4^E133X, HPS4^Q259X, HPS4^W515X or HPS4^Q664X (using the "eyFLP/FRT" system).

The lighter colour of w^IR.GMR eyes is partially suppressed when eyes are also made homozygous mutant for FBX011^Q803R or Dcr-1^Q1147X (using the "eyFLP/FRT" system). w^IR.GMR, Dcr-1^Q1147X are also smaller than w^IR.GMR eyes.

w^IR.GMR eyes made homozygous for both FBX011^Q803R and Dcr-1^Q1147X (using the "eyFLP/FRT" system) show a greater suppression of the eye pigmentation phenotype but an enhancement of the small eye phenotype seen in w^IR.GMR, Dcr-1^Q1147X eyes.

(Lee et al., 2009)

w^IR.GMR eyes that are also homozygous for Dcr-2^L811fsX show strong suppression of the pale eye colour phenotype. w^IR.GMR eyes that are also homozygous for Dcr-2^P8S, Dcr-2^A1453T, Dcr-2^R269W, Dcr-2^P365L, Dcr-2^P1496L or Dcr-2^L188F show stronger eye pigmentation compared to w^IR.GMR eyes.

(Lim et al., 2008)

Flies carrying w^IR.GMR in which the eyes are homozygous for Dcr-2^L811fsX have dark red eyes.

Flies carrying w^IR.GMR in which the eyes are homozygous for bel⁶ have small, rough eyes. The eyes contain patches of increased pigmentation compared to w^IR.GMR single mutant flies.

Flies carrying w^IR.GMR in which the eyes are homozygous for Hsc70-4^54.1 have small, rough eyes. There is no increase in eye pigmentation compared to w^IR.GMR single mutant flies.

(Zhou et al., 2008)

Adult flies with one copy of w^IR.GMR and AGO2^V966M exhibit a pale orange eye colour.

Adult flies with two copies of w^IR.GMR and that are homozygous for AGO2^V966M exhibit a relatively wild-type eye colouration.

Adult flies with two copies of w^IR.GMR and that are homozygous for AGO2⁴¹⁴ exhibit a relatively wild-type eye colouration.

Adult flies with two copies of w^IR.GMR and that are transheterozygous for AGO2⁴¹⁴ and AGO2^V966M exhibit a relatively wild-type eye colouration.

Adult flies with two copies of w^IR.GMR and that are transheterozygous for AGO2⁴¹⁴ and AGO2^V966M exhibit a pale orange eye colouration in the presence of AGO2^+t10.1.

(Kim et al., 2007)

Flies carrying one copy of w^dsRNA.GMR in a homozygous Dcr-2^L811fsX background have red eyes.

The yellowish eye colour caused by expression of one copy of w^dsRNA.GMR in a wild-type background is not altered if the flies are also homozygous for either piwi¹ or piwi⁰⁶⁸⁴³ or are carrying aub^HN2/aub^N11.

(Pelisson et al., 2007)

Dcr-2^unspecified/Dcr-2^unspecified substantially restores eye pigmentation in flies carrying w^dsRNA.GMR.

Snp^c00465/Snp^c00465 does not enhance the loss of eye pigmentation seen in flies carrying w^dsRNA.GMR.

(Kupsco et al., 2006)

The silencing of the w gene seen in flies carrying P{GMR-wIR} is suppressed by r2d2^unspecified/r2d2^unspecified; resulting in flies with red eyes. The silencing of the w gene seen in flies carrying P{GMR-wIR} is less efficient if the flies are also homozygous for loqs^f00791; flies carrying two copies of P{GMR-wIR} and homozygous for loqs^f00791 do not have completely white eyes (in contrast to flies carrying two copies of P{GMR-wIR} in a wild-type background).

(Forstemann et al., 2005)

Dcr-1^Q1147X, w^IR.GMR animals have eyes that have reduced pigment, and are also reduced in size. The organisation of ommatidial facets is also disrupted and sensory bristles are missing over the eye surface. Other bristles, which flank the eye surface, are sometimes absent or exhibit hyperplasia.

(Lee et al., 2004)

Xenogenetic Interactions

Statement

Reference

Eye colour: the reduced eye pigmentation seen in flies expressing DCV is suppressed by expression of DCV\1A^{Scer\UAS.P\T.cvRa} under the control of Scer\GAL4^GMR.PF.

Eye colour: the reduced eye pigmentation seen in flies expressing DCV is not suppressed by expression of DCV\1A^{KK73AA.Scer\UAS.P\T} under the control of Scer\GAL4^GMR.PF.

(van Rij et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

32068

P{GMR-w.RNAi}13D; P{neoFRT}40A r2d2^S165fsX

32067

P{GMR-w.RNAi}13D; P{neoFRT}82B Dcr-1^Q1948X/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Transfected into flies to study the phenotypic consequences of dsRNA interference (RNAi) of the w gene.

(Lee and Carthew, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

w^IR.GMR

w^RNAi.GMR

w^dsRNA.GMR

Name Synonyms

Secondary FlyBase IDs

FBal0191476
FBal0154368

References (32)

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