FlyBase Recombinant Construct Report: P{GMR-w.RNAi}

General Information

Symbol

P{GMR-w.RNAi}

FlyBase ID

FBtp0070260

Feature type

transgenic_transposable_element

Component Allele(s)

w^RNAi.GMR

Also Known As

GMR-wIR, GMR-w^IR

Size

Expression Data

Associated insertion(s)

4 available

Molecular map

Description and Uses

Construct components

Component allele w^RNAi.GMR

Transgenic product class

RNAi_reagent

(Lee et al., 2004, Lee and Carthew, 2003)

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Description

The GMR promoter drives expression of a tail-to-tail w repeat. The repeat comprises the 629bp third exon of w ligated to the 74bp second intron of w, ligated to another copy of the w third exon in the opposite orientation to the first copy.

(Lee and Carthew, 2003)

Consists of two copies of the w exon 3 in opposite orientation to each other and separated by a w intron. The construct is terminated with a Hsp70 terminator. The resulting transcript after intron splicing, is predicted to create a perfect hairpin dsRNA of length 629 bp.

(Lee et al., 2004)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

w^RNAi.GMR

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

32068

P{GMR-w.RNAi}13D; P{neoFRT}40A r2d2^S165fsX

32067

P{GMR-w.RNAi}13D; P{neoFRT}82B Dcr-1^Q1948X/TM3, Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

GMR-w-RNAi

(Nitta et al., 2023)

GMR-w.IR

(Sun et al., 2024)

GMR-w^IR

(Donelick et al., 2020, Ji et al., 2012)

GMR-wIR

(Lee et al., 2020, Lei and Chinen, 2020.6.24, Lee et al., 2018, Chinen and Lei, 2017, Kandasamy et al., 2017, Kandasamy and Fukunaga, 2016, Lim et al., 2014, Fukunaga et al., 2012, Harris et al., 2011, Schneiderman et al., 2010, Lee et al., 2009, Lim et al., 2008, Zhou et al., 2008, Kim et al., 2007, Pelisson et al., 2007, Lee et al., 2004)

GMR>IR^w

(Berry et al., 2009)

GMRwIR

(Kupsco et al., 2006)

GMRwhite RNAi

(Richard et al., 2022)

IR-white

(Forstemann et al., 2005)

IR^white

(Nayak et al., 2010)

P[GMR-whiteIR]

(Lee and Carthew, 2003)

P{GMR-w.IR}

(FlyBase, 1996-)

P{GMR-w.RNAi}

(FlyBase, 1996-)

P{GMR-wIR}

(van Rij et al., 2006, FlyBase, 1996-)

p[GMR-wIR]

(Lim et al., 2014)

Secondary FlyBase IDs

FBtp0020657
FBtp0018219

References (33)

Report Sections

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