FlyBase Allele Report: Dmel\dar1[UAS.Tag:V5,Tag:polyHis]

General Information

Symbol

Dmel\dar1^{UAS.Tag:V5,Tag:polyHis}

Species

D. melanogaster

Name

Saccharomyces cerevisiae construct a of Ye

FlyBase ID

FBal0258678

Feature type

allele

Associated gene

Dmel\dar1

Associated Insertion(s)

Carried in Construct

P{UAS-dar1.Y}

Also Known As

UAS-Dar1

Key Links

Transgenic product class

wild_type

(Ye, 2016.9.6)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ye, 2016.9.6)

Mutagen

in vitro construct

(Ye, 2016.9.6, Ye et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-dar1.Y}

Cytology

Description

UAS regulatory sequences drive expression of dar1 tagged at the C-terminal end with Tag:V5 and Tag:polyHis.

(Ye, 2016.9.6)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷

(Ye et al., 2011)

abnormal neuroanatomy, with Scer\GAL4^eve.RN2

(Wang et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^P2.4.Pdf

(Wang et al., 2015)

abnormal neuroanatomy | somatic clone, with Scer\GAL4⁴⁷⁷

(Ye et al., 2011)

Phenotype Manifest In

adult Pdf neuron, with Scer\GAL4^P2.4.Pdf

(Wang et al., 2015)

dendrite | increased number, with Scer\GAL4^eve.RN2

(Wang et al., 2015)

dendrite | increased number, with Scer\GAL4^P2.4.Pdf

(Wang et al., 2015)

dendritic arborizing neuron, with Scer\GAL4⁴⁷⁷

(Ye et al., 2011)

dendritic arborizing neuron | somatic clone, with Scer\GAL4⁴⁷⁷

(Ye et al., 2011)

embryonic/larval motor neuron, with Scer\GAL4^eve.RN2

(Wang et al., 2015)

neurite | increased number, with Scer\GAL4^eve.RN2

(Wang et al., 2015)

Detailed Description

Statement

Reference

Expression of dar1^Scer\UAS.cYa under the control of Scer\GAL4^eve.RN2 (in unipolar motor neurons) leads to supernumerary neurites extending from the soma, and subsequent multipolar morphology of those neurons.

Expression of dar1^Scer\UAS.cYa under the control of Scer\GAL4^P2.4.Pdf (in adult unipolar PDF neurons) or in larval bipolar es neurons using Scer\GAL4^unspecified leads to an increased number of primary dendrites, and multipolar neuron morphology.

(Wang et al., 2015)

Overexpression of dar1^Scer\UAS.cYa with the driver Scer\GAL4⁴⁷⁷ causes dramatic overgrowth of dendrites in the class IV da neurons in early third-instar larvae. Similarly, the total dendritic length of these neurons is increased compared to controls. Sholl analysis shows that the increase in branch number and length takes place throughout the dendritic fields rather than being restricted to particular regions. No change in the size of dendritic fields or dendritic tiling is observed.

Using the Flip-out technique, overexpression of dar1^Scer\UAS.cYa in class IV da neuron or class I da neuron axons under the control of Scer\GAL4⁴⁷⁷ does not significantly affect axon growth. Overexpression in class III da neurons increases the number of long curvy terminal branches.

(Ye et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has abnormal neuroanatomy phenotype, suppressible | partially by sw¹/sw[+]

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has abnormal neuroanatomy phenotype, suppressible | partially by nudE[+]/nudE^11R2

(Wang et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has neurite | increased number phenotype, suppressible | partially by sw¹/sw[+]

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has dendrite | increased number phenotype, suppressible | partially by sw¹/sw[+]

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has neurite | increased number phenotype, suppressible | partially by Scer\GAL4^eve.RN2/nudC^HMS00258

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has dendrite | increased number phenotype, suppressible | partially by Scer\GAL4^eve.RN2/nudC^HMS00258

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has embryonic/larval motor neuron phenotype, suppressible | partially by Scer\GAL4^eve.RN2/nudC^HMS00258

(Wang et al., 2015)

Scer\GAL4^P2.4.Pdf, dar1^{UAS.Tag:V5,Tag:polyHis} has dendrite | increased number phenotype, suppressible | partially by sw¹/sw[+]

(Wang et al., 2015)

Scer\GAL4^P2.4.Pdf, dar1^{UAS.Tag:V5,Tag:polyHis} has adult Pdf neuron phenotype, suppressible | partially by sw¹/sw[+]

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has embryonic/larval motor neuron phenotype, suppressible | partially by sw¹/sw[+]

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has neurite | increased number phenotype, suppressible | partially by nudE[+]/nudE^11R2

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has dendrite | increased number phenotype, suppressible | partially by nudE[+]/nudE^11R2

(Wang et al., 2015)

Scer\GAL4^P2.4.Pdf, dar1^{UAS.Tag:V5,Tag:polyHis} has dendrite | increased number phenotype, suppressible | partially by nudE[+]/nudE^11R2

(Wang et al., 2015)

Scer\GAL4^P2.4.Pdf, dar1^{UAS.Tag:V5,Tag:polyHis} has adult Pdf neuron phenotype, suppressible | partially by nudE[+]/nudE^11R2

(Wang et al., 2015)

Scer\GAL4^eve.RN2, dar1^{UAS.Tag:V5,Tag:polyHis} has embryonic/larval motor neuron phenotype, suppressible | partially by nudE[+]/nudE^11R2

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The supernumerary dendrite phenotype seen in animals expressing dar1^Scer\UAS.cYa under the control of Scer\GAL4^eve.RN2 (in unipolar motor neurons) or Scer\GAL4^P2.4.Pdf (in adult unipolar PDF neurons) is greatly reduced in sw¹/+ mutants and nudE^11R2/+ mutants.

Coexpression of nudC^HMS00258 partially rescues the supernumerary dendrite phenotype seen in animals expressing dar1^Scer\UAS.cYa under the control of Scer\GAL4^eve.RN2 (in unipolar motor neurons).

(Wang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65261

w¹¹¹⁸; P{UAS-dar1.Y}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

dar1^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}

dar1^Scer\UAS.cYa

dar1^{UAS.Tag:V5,Tag:polyHis}

Name Synonyms

Saccharomyces cerevisiae construct a of Ye

Saccharyomyces cerevisiae construct a of Ye

Secondary FlyBase IDs

References (4)

Report Sections

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