FlyBase Allele Report: Dmel\piwi[HMS00606]

Expression of piwi^HMS00606 under the control of Scer\GAL4^GMR.PU does not result in any obvious phenotype in the adult eye or third instar larval wing disc, as compared to controls.

(Zhang et al., 2015)

The expression of piwi^HMS00606 under the control of Scer\GAL4^C587 at room temperature leads to germarium defects as compared to controls: germ line stem cells range from completely absent to increased in number, some locating away from cap cells; escort cells are severely decreased in number, show increases in DNA damage and in apoptosis, and lose their long cellular processes. Similar germ line cell defects are observed in adults kept at 18[o]C throughout development and shifted to 29[o]C upon eclosion, but not in adults kept continuously at 18[o]C throughout development and adulthood.

The expression of piwi^HMS00606 under the control of Scer\GAL4^VP16.nos.UTR results in moderate to severe decreases in the number of primordial germ cells in the third instar larval female gonad and results in a complete loss of germ line stem cells in the germarium of newly eclosed adults, as compared to controls.

The clonal expression of piwi^HMS00606 under the control of Scer\GAL4^{VP16.nos.-FRT} in the germarium leads to the progressive loss of germline stem cells within the clones (a significant proportion of germaria lack germ line stem cells 3 weeks after clone induction, but not 1 week after clone induction), as compared to controls; conversely, some of these germaria accumulate excessive germline stem cells outside of the clone region.

(Ma et al., 2014)

Females expressing piwi^HMS00606 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} do not show gross morphological defects in the ovary, but are sterile.

(Le Thomas et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has abnormal cell number | oogenesis phenotype, non-enhanceable by P{UAS-copia.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has abnormal cell number | oogenesis phenotype, non-enhanceable by lok^GD4190/Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has abnormal cell number | oogenesis phenotype, suppressible by P{UAS-gypsy.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^C587, piwi^HMS00606 has increased cell number | oogenesis phenotype, suppressible | partially by Scer\GAL4^C587/dpp^JF01371

(Ma et al., 2014)

Scer\GAL4^C587, piwi^HMS00606 has increased cell number | oogenesis phenotype, suppressible | partially by Scer\GAL4^C587/dpp^HMS00011

(Ma et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has abnormal cell number | oogenesis phenotype, non-suppressible by P{UAS-copia.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has abnormal cell number | oogenesis phenotype, non-suppressible by lok^GD4190/Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Suppressor of

Statement

Reference

piwi^HMS00606/Scer\GAL4^GMR.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP}

(Zhang et al., 2015)

piwi^HMS00606/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP}

(Zhang et al., 2015)

piwi^HMS00606/Scer\GAL4^en.PU is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^en.PU, yki^UAS.cHa

(Zhang et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has female germline stem cell phenotype, non-enhanceable by P{UAS-copia.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has germarium phenotype, non-enhanceable by P{UAS-copia.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has female germline stem cell phenotype, non-enhanceable by lok^GD4190/Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has germarium phenotype, non-enhanceable by lok^GD4190/Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has female germline stem cell phenotype, suppressible by P{UAS-gypsy.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has germarium phenotype, suppressible | partially by P{UAS-gypsy.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^C587, piwi^HMS00606 has germarium phenotype, suppressible | partially by Scer\GAL4^C587/dpp^JF01371

(Ma et al., 2014)

Scer\GAL4^C587, piwi^HMS00606 has female germline stem cell phenotype, suppressible | partially by Scer\GAL4^C587/dpp^JF01371

(Ma et al., 2014)

Scer\GAL4^C587, piwi^HMS00606 has germarium phenotype, suppressible | partially by Scer\GAL4^C587/dpp^HMS00011

(Ma et al., 2014)

Scer\GAL4^C587, piwi^HMS00606 has female germline stem cell phenotype, suppressible | partially by Scer\GAL4^C587/dpp^HMS00011

(Ma et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has female germline stem cell phenotype, non-suppressible by P{UAS-copia.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has germarium phenotype, non-suppressible by P{UAS-copia.RNAi}, Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has female germline stem cell phenotype, non-suppressible by lok^GD4190/Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^HMS00606 has germarium phenotype, non-suppressible by lok^GD4190/Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Suppressor of

Statement

Reference

piwi^HMS00606/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP}

(Zhang et al., 2015)

piwi^HMS00606/Scer\GAL4^en.PU is a suppressor | partially of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en.PU, yki^UAS.cHa

(Zhang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of piwi^HMS00606 rescues the eye overgrowth seen in adults expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU.

Co-expression of piwi^HMS00606 partially suppresses the wing disc posterior compartment overgrowth of larvae expressing yki^Scer\UAS.cHa under the control of Scer\GAL4^en.PU.

(Zhang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33724

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00606}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

piwi^HMS00606

Name Synonyms

Secondary FlyBase IDs

References (17)

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