FB2024_03 , released June 25, 2024
Allele: Hsap\SCRIBUAS.EGFP
Open Close
General Information
Symbol
Hsap\SCRIBUAS.EGFP
Species
H. sapiens
Name
FlyBase ID
FBal0256743
Feature type
allele
Associated gene
Hsap\SCRIB
Associated Insertion(s)
Carried in Construct
P{UAS-EGFP-hScrib}
Key Links
Transgenic product class
wild_type
(Dow et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Dow et al., 2003)
Mutagen
in vitro construct
(Dow et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-EGFP-hScrib}
Cytology
Description

UAS regulatory sequences drive expression of N-terminally tagged full-length Hsap\SCRIB coding sequences (composed of GenBank accessions D63481 and NM_015356).

(Dow et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\SCRIB
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA with scribj7B3
      (Dow et al., 2003)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP within wild-type clones of cells (under the control of Scer\GAL4αTub84B.PL) does not affect differentiation.

      (Dow et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Scer\GAL4Act.PU/Hsap\SCRIBUAS.EGFP is a suppressor of lethal | larval stage phenotype of scrib1/scribj7B3

      (Dow et al., 2003)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4αTub84B.PL) within MARCM-generated scrib1 clones largely restores the differentiation and patterning of the photoreceptors in the eye. Hsap\ScribScer\UAS.T:Avic\GFP-EGFP expression also suppresses the tissue organisation defects associated with scrib1 mutant tissue.

      scrib1 mutant clones expressing Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4αTub84B.PL) restores the columnar architecture and monolayer organization of the epithelial cells, while the cytoskeleton appears normal.

      Ubiquitous expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4Act.PU) effectively suppresses the larval overgrowth phenotype found in scrib1/scribj7B3 mutants. Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP in scrib1/scribj7B3 mutant third instar imaginal wings suppresses the tumorous overgrowth of the epithelial tissues and allows the formation of a monolayered epithelial structure closely resembling that of wild-type discs.

      Expression of Hsap\ScribScer\UAS.T:Avic\GFP-EGFP (under the control of Scer\GAL4Act.PU) rescues larval lethality in scrib1/scribj7B3 mutants to allow 59% to form adult structures (pharate adults) and 23% to eclose as mature adult flies. These adult flies exhibit some developmental defects in the eyes and wings on a proportion of rescued animals as well as ectopic scutellar bristles, suggesting the rescue effect is not fully penetrant. Both sexes appear to be sterile.

      (Dow et al., 2003)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\SCRIBScer\UAS.T:Avic\GFP-EGFP
      Hsap\SCRIBUAS.EGFP
      Hsap\ScribScer\UAS.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)