FlyBase Allele Report: Dmel\hh[HMS00492]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\hh^HMS00492

General Information

Symbol

Dmel\hh^HMS00492

Species

D. melanogaster

Name

FlyBase ID

FBal0248244

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00492}

Also Known As

UAS-hh^RNAi

Key Links

Genomic Maps

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00492}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^hh-Gal4

(Kim et al., 2014)

visible, with Scer\GAL4^hh-Gal4

(Kim et al., 2014)

Phenotype Manifest In

egg chamber, with Scer\GAL4^109-30

(Sahai-Hernandez and Nystul, 2013)

egg chamber, with Scer\GAL4^bab1-Agal4-5, Scer\GAL4^tj.PU

(Sahai-Hernandez and Nystul, 2013)

egg chamber, with Scer\GAL4^GMR13C06

(Sahai-Hernandez and Nystul, 2013)

egg chamber, with Scer\GAL4^tj.PU

(Sahai-Hernandez and Nystul, 2013)

epithelium | female, with Scer\GAL4^bab1-Agal4-5

(Sahai-Hernandez and Nystul, 2013)

epithelium | female, with Scer\GAL4^GMR13C06

(Sahai-Hernandez and Nystul, 2013)

germarium, with Scer\GAL4^109-30

(Sahai-Hernandez and Nystul, 2013)

germarium, with Scer\GAL4^bab1-Agal4-5

(Sahai-Hernandez and Nystul, 2013)

germarium, with Scer\GAL4^GMR13C06

(Sahai-Hernandez and Nystul, 2013)

ovariole, with Scer\GAL4^109-30

(Sahai-Hernandez and Nystul, 2013)

ovariole, with Scer\GAL4^bab1-Agal4-5

(Sahai-Hernandez and Nystul, 2013)

ovariole, with Scer\GAL4^bab1-Agal4-5, Scer\GAL4^tj.PU

(Sahai-Hernandez and Nystul, 2013)

ovariole, with Scer\GAL4^GMR13C06

(Sahai-Hernandez and Nystul, 2013)

ovariole, with Scer\GAL4^tj.PU

(Sahai-Hernandez and Nystul, 2013)

wing blade, with Scer\GAL4^hh-Gal4

(Kim et al., 2014)

wing blade anterior compartment, with Scer\GAL4^hh-Gal4

(Kim et al., 2014)

wing blade posterior compartment, with Scer\GAL4^hh-Gal4

(Kim et al., 2014)

Detailed Description

Statement

Reference

Under starvation conditions, the adulthood-only expression of hh^HMS00492 under the control of Scer\GAL4^bab1-Pgal4-2 (and Gal80[ts] for temporal control of expression) does not significantly affect the mitotic index of the follicular epithelium (phospho-H3 immunofluorescence), as compared to controls.

(Çiçek et al., 2016)

Expression of hh^HMS00492 driven by Scer\GAL4^hh-Gal4 significantly decreases posterior (P) and anterior (A) wing compartment size (P/A ratio remains similar to controls); there is slight curving of the wing toward the posterior compartment.

(Kim et al., 2014)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^hh-Gal4, hh^HMS00492 has visible phenotype, suppressible by mir-14^Δ1/mir-14^Δ1

(Kim et al., 2014)

hh^HMS00492 has abnormal size | adult stage phenotype, suppressible by mir-14^Δ1/mir-14^Δ1

(Kim et al., 2014)

Suppressor of

Statement

Reference

hh^HMS00492/Scer\GAL4^hh-Gal4 is a suppressor | partially of visible phenotype of mir-14^Δ1

(Kim et al., 2014)

hh^HMS00492/Scer\GAL4^hh-Gal4 is a suppressor | partially of abnormal size | adult stage phenotype of mir-14^Δ1

(Kim et al., 2014)

hh^HMS00492/Scer\GAL4^hh-Gal4 is a suppressor of increased cell number | third instar larval stage phenotype of mir-14^Δ1

(Kim et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^hh-Gal4, hh^HMS00492 has wing blade phenotype, suppressible by mir-14^Δ1/mir-14^Δ1

(Kim et al., 2014)

Scer\GAL4^hh-Gal4, hh^HMS00492 has wing blade posterior compartment phenotype, suppressible by mir-14^Δ1/mir-14^Δ1

(Kim et al., 2014)

Suppressor of

Statement

Reference

hh^HMS00492/Scer\GAL4^hh-Gal4 is a suppressor | partially of wing blade phenotype of mir-14^Δ1

(Kim et al., 2014)

hh^HMS00492/Scer\GAL4^hh-Gal4 is a suppressor of embryonic/larval dorsal tracheal branch | third instar larval stage phenotype of mir-14^Δ1

(Kim et al., 2014)

hh^HMS00492/Scer\GAL4^hh-Gal4 is a suppressor of terminal tracheal cell | third instar larval stage phenotype of mir-14^Δ1

(Kim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

mir-14^Δ1/mir-14^Δ1 significantly suppresses the wing curvature and decreases in posterior wing compartment size seen in Scer\GAL4^hh-Gal4>hh^HMS00492 flies; these flies actually show a significant increase in posterior wing compartment size (and P/A wing size ratio) compared to controls. Expression of hh^HMS00492 driven by Scer\GAL4^hh-Gal4 significantly (partially) suppresses the increases in wing size seen in mir-14^Δ1 mutants.

Expression of hh^HMS00492 driven by Scer\GAL4^hh-Gal4 significantly suppresses the increased percentage of mir-14^Δ1/mir-14^Δ1 mutant dorsal tracheal branches that have excess (>2) terminal cells.

(Kim et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

32489

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00492}attP2/TM3, Sb¹

HMS00492

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00492}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hh^HMS00492

hh^RNAi

(Velarde et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections