UAS regulatory sequences drive expression of a short inverted repeat.
Under starvation conditions, the adulthood-only expression of hhHMS00492 under the control of Scer\GAL4bab1-Pgal4-2 (and Gal80[ts] for temporal control of expression) does not significantly affect the mitotic index of the follicular epithelium (phospho-H3 immunofluorescence), as compared to controls.
Expression of hhHMS00492 driven by Scer\GAL4hh-Gal4 significantly decreases posterior (P) and anterior (A) wing compartment size (P/A ratio remains similar to controls); there is slight curving of the wing toward the posterior compartment.
Scer\GAL4hh-Gal4, hhHMS00492 has visible phenotype, suppressible by mir-14Δ1/mir-14Δ1
hhHMS00492 has abnormal size | adult stage phenotype, suppressible by mir-14Δ1/mir-14Δ1
hhHMS00492/Scer\GAL4hh-Gal4 is a suppressor | partially of visible phenotype of mir-14Δ1
hhHMS00492/Scer\GAL4hh-Gal4 is a suppressor | partially of abnormal size | adult stage phenotype of mir-14Δ1
hhHMS00492/Scer\GAL4hh-Gal4 is a suppressor of increased cell number | third instar larval stage phenotype of mir-14Δ1
Scer\GAL4hh-Gal4, hhHMS00492 has wing blade phenotype, suppressible by mir-14Δ1/mir-14Δ1
Scer\GAL4hh-Gal4, hhHMS00492 has wing blade posterior compartment phenotype, suppressible by mir-14Δ1/mir-14Δ1
hhHMS00492/Scer\GAL4hh-Gal4 is a suppressor | partially of wing blade phenotype of mir-14Δ1
hhHMS00492/Scer\GAL4hh-Gal4 is a suppressor of embryonic/larval dorsal tracheal branch | third instar larval stage phenotype of mir-14Δ1
hhHMS00492/Scer\GAL4hh-Gal4 is a suppressor of terminal tracheal cell | third instar larval stage phenotype of mir-14Δ1
mir-14Δ1/mir-14Δ1 significantly suppresses the wing curvature and decreases in posterior wing compartment size seen in Scer\GAL4hh-Gal4>hhHMS00492 flies; these flies actually show a significant increase in posterior wing compartment size (and P/A wing size ratio) compared to controls. Expression of hhHMS00492 driven by Scer\GAL4hh-Gal4 significantly (partially) suppresses the increases in wing size seen in mir-14Δ1 mutants.
Expression of hhHMS00492 driven by Scer\GAL4hh-Gal4 significantly suppresses the increased percentage of mir-14Δ1/mir-14Δ1 mutant dorsal tracheal branches that have excess (>2) terminal cells.