FlyBase Allele Report: Dmel\Egfr[U.A887T.UAS]

General Information

Symbol

Dmel\Egfr^U.A887T.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0246628

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{UAS-Egfr.U.A887T}

Also Known As

UAS-EGFR^A887T

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Yan et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Egfr.U.A887T}

Cytology

Description

UAS regulatory sequences drive expression of activated Egfr.

(Yan et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cardiomyopathy

CEA

(Yu et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | male, with Scer\GAL4^C587

(Eun et al., 2014)

increased cell size, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Nie et al., 2015)

visible, with Scer\GAL4^bbg-C96

(Settle et al., 2003)

visible, with Scer\GAL4^GMR.PU

(Yan et al., 2010)

Phenotype Manifest In

adult heart, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

adult heart muscle cell, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

adult midgut, with Scer\GAL4^NP5130

(Nie et al., 2015)

cardial cell, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

eye, with Scer\GAL4^GMR.PU

(Yan et al., 2010)

midline glial cell | embryonic stage 16 | increased number, with Scer\GAL4^sim.P3.7

(Settle et al., 2003)

myofilament, with Scer\GAL4^tin.CΔ4

(Yu et al., 2013)

posterior wing margin, with Scer\GAL4^bbg-C96

(Settle et al., 2003)

testis, with Scer\GAL4^C587

(Eun et al., 2014)

Detailed Description

Statement

Reference

Expression of Egfr^{U.A887T.Scer\UAS} under the control of Scer\GAL4^esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant increase in the number of mitotic cells in the midgut, as compared to controls.

(Nie et al., 2015)

Adult Scer\GAL4^tin.CΔ4>Egfr^{U.A887T.Scer\UAS} flies have smaller heart chambers with reduced end-diastolic dimensions compared with controls. Histological analysis of cardiac chambers from the adult transgenic flies show heart wall thicknesses that are two to three times compared with controls. Hearts isolated from the transgenic flies show abnormal cardiac morphology with myofiber disarray compared with controls. The number of cells in the transgenic hearts is similar to that in wild-type, indicating that the increased wall thickness is the result of cardiomyocyte hypertrophy, not an increase in cell proliferation.

(Yu et al., 2013)

Expression of Egfr^{U.A887T.Scer\UAS} in differentiating eye cells driven by Scer\GAL4^GMR.PU causes rough eyes of reduced size.

(Yan et al., 2010)

Expression of Egfr^{U.A887T.Scer\UAS} under the control of Scer\GAL4^sim.P3.7 results in an increased number of midline glia cells compared to wild type at stage 16 of embryogenesis.

Flies expressing Egfr^{U.A887T.Scer\UAS} under the control of Scer\GAL4^bbg-C96 have notches on the posterior half of the wing margin.

(Settle et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Egfr^U.A887T.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU

(Yan et al., 2010)

Other

Statement

Reference

Egfr^U.A887T.UAS, Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU has lethal phenotype

(Yan et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^U.A887T.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU

(Yan et al., 2010)

NOT suppressed by

Statement

Reference

Egfr^U.A887T.UAS, Scer\GAL4^sim.P3.7 has midline glial cell | increased number | embryonic stage 16 phenotype, non-suppressible by Rat\Neu^KDYD.UAS, Scer\GAL4^sim.P3.7

(Settle et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of bun^GD1392 or Madm^GD7155 suppresses the increased number of mitotic cells seen in the midguts of flies expressing Egfr^{U.A887T.Scer\UAS} under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

Co-expression of Rabex-5^VDRC.cUa significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Egfr^{U.A887T.Scer\UAS} driven by Scer\GAL4^GMR.PU.

(Yan et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of Rat\Neu^{KDYD.Scer\UAS} does not significantly reduce the increased number of midline glia cells at stage 16 of embryogenesis which are seen in animals expressing Egfr^{U.A887T.Scer\UAS} under the control of Scer\GAL4^sim.P3.7.

(Settle et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

This line is originally from the Bloomington Stock Center, so it could correspond to either Egfr^{1.A887T.Scer\UAS} or Egfr^{2.A887T.Scer\UAS}.

(Yan et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DEgfr^A887T

(Settle et al., 2003)

Egfr^{U.A887T.Scer\UAS}

Egfr^U.A887T.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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