FlyBase Allele Report: Dmel\klhl10[UAS.cKa]

General Information

Symbol

Dmel\klhl10^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kaplan

FlyBase ID

FBal0246328

Feature type

allele

Associated gene

Dmel\klhl10

Associated Insertion(s)

Carried in Construct

P{UAS-klhl10.K}

Key Links

Transgenic product class

wild_type

(Kaplan et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kaplan et al., 2010)

Mutagen

in vitro construct

(Kaplan et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-klhl10.K}

Cytology

Description

UAS regulatory sequences drive expression of klhl10.

(Kaplan et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

klhl10

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^GMR.PS

(Aram et al., 2016)

visible, with Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Aram et al., 2016)

eye, with Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

ommatidium, with Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

Detailed Description

Statement

Reference

Expression of klhl10^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PS results in reduced eye size.

(Aram et al., 2016)

Expression of klhl10^Scer\UAS.cKa in the eye driven by Scer\GAL4^GMR.PU results in highly reduced eye size with disorganised ommatidia.

(Kaplan et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has abnormal size phenotype, enhanceable by Cul3^UAS.T, Scer\GAL4^GMR.PS

(Aram et al., 2016)

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has abnormal size phenotype, enhanceable by Scer\GAL4^GMR.PS/ScsβA^UAS.T

(Aram et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has abnormal size phenotype, suppressible | partially by Cul3[+]/Cul3⁰⁶⁴³⁰

(Aram et al., 2016)

Scer\GAL4^GMR.PU, klhl10^UAS.cKa has visible phenotype, suppressible by soti^UAS.cKa/Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has abnormal size phenotype, non-suppressible by Cul3^mds1/Cul3[+]

(Aram et al., 2016)

Other

Statement

Reference

Cul3^UAS.T, Scer\GAL4^GMR.PS, ScsβA^UAS.T, klhl10^UAS.cKa has lethal - all die before end of P-stage phenotype

(Aram et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has eye phenotype, enhanceable by Cul3^UAS.T, Scer\GAL4^GMR.PS

(Aram et al., 2016)

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has eye phenotype, enhanceable by Scer\GAL4^GMR.PS/ScsβA^UAS.T

(Aram et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has eye phenotype, suppressible | partially by Cul3[+]/Cul3⁰⁶⁴³⁰

(Aram et al., 2016)

Scer\GAL4^GMR.PU, klhl10^UAS.cKa has eye phenotype, suppressible by soti^UAS.cKa/Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

Scer\GAL4^GMR.PU, klhl10^UAS.cKa has ommatidium phenotype, suppressible by soti^UAS.cKa/Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PS, klhl10^UAS.cKa has eye phenotype, non-suppressible by Cul3^mds1/Cul3[+]

(Aram et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of skap^Scer\UAS.T or Cul3^Scer\UAS.T enhances the reduced eye phenotype of flies expressing klhl10^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PS.

Cul3⁰⁶⁴³⁰/+, but not Cul3^mds1/+, partially suppresses the reduced eye phenotype of flies expressing klhl10^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PS.

Coexpression of Cul3^Scer\UAS.T, skap^Scer\UAS.T and klhl10^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PS results in lethality, with flies being unable to eclose.

(Aram et al., 2016)

Co-expression of soti^Scer\UAS.cKa completely suppresses the eye phenotype resulting from the expression of klhl10^Scer\UAS.cKa driven by Scer\GAL4^GMR.PU

(Kaplan et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

klhl10^Scer\UAS.cKa

klhl10^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kaplan

Secondary FlyBase IDs

References (2)

Report Sections

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