Expression of Pvf2UAS.cMa under the control of Scer\GAL4Hml.Δ leads to lethality in animals raised on poor diet.
Expression of Pvf2UAS.cMa under the control of Scer\GAL4Hml.Δ leads to smaller lipid droplets with reduced intensity in fat body of larvae.
Expression of Pvf2Scer\UAS.cMa under the control of Scer\GAL4crq.PO does not perturb hemocyte distribution in embryos.
Expression of Pvf2Scer\UAS.cMa under the control of either Scer\GAL4da.G32 or Scer\GAL4e33C results in a dramatic increase in the number of hemocytes in third instar larvae.
Crystal cells are absent in larvae expressing Pvf2Scer\UAS.cMa under the control of Scer\GAL4e33C. The blood cells that are present are prohemocytes or plasmatocytes. Only 5% of the blood cells are fully mature plasmatocytes (assayed by their ability to phagocytose injected India ink) in the mutant larvae, compared to 80% in controls.
Pvf2UAS.cMa/Scer\GAL4crq.PO partially rescues Df(2L)Pvf2-3
Expression of Pvf2Scer\UAS.cMa under the control of Scer\GAL4crq.PO partially rescues the embryonic lethality of Df(2L)Pvf2-3/Df(2L)Pvf2-3 mutants and fully rescues the decrease in hemocyte numbers observed in stage 13 mutant embryos as well as the perturbed posterior migration of ventral hemocytes but fails to restore the invasion of the extended germband in mutant embryos.
Expression of Pvf2Scer\UAS.cMa under the control of Scer\GAL4byn-Gal4 rescues the inability of hemocytes in the Df(2L)Pvf2-3 mutant embryos to penetrate the extended germband and restores their invasive migration.
