UASp regulatory sequences drive expression of a mutated form of Dg in which both the SH3bsI and SH3bsII protein binding sites in the proline-rich C-terminus have been disrupted by proline to alanine substitutions.
Scer\GAL4VP16.nanos.UTR/DgPA.UASp fails to rescue Dg323
Expression of DgPA.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 does not rescue the oocyte polarity and egg chamber growth defects of Dg323 mutants.