FlyBase Allele Report: Dmel\Dys[8-2]

General Information

Symbol

Dmel\Dys^8-2

Species

D. melanogaster

Name

FlyBase ID

FBal0244097

Feature type

allele

Associated gene

Dmel\Dys

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Shcherbata et al., 2007)

Progenitor genotype

Dys^EP3397

(Shcherbata et al., 2007)

Cytology

Description

Imprecise excision of P{EP}Dys^EP3397 resulting in a small deletion.

(Shcherbata et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of muscular dystrophy

CEA

(Shcherbata et al., 2007)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Dys^8-2 exhibits muscular dystrophy-like phenotypes when in combination with Df(3R)Dl-X43

(Shcherbata et al., 2007)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

abnormal locomotor behavior | adult stage | conditional (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

abnormal neuroanatomy | third instar larval stage (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

abnormal neuroanatomy | third instar larval stage (with Dys^E6)

(Shcherbata et al., 2007)

Phenotype Manifest In

adult heart chamber (with Df(3R)Delta-X43)

(Taghli-Lamallem et al., 2008)

adult heart muscle cell (with Df(3R)Delta-X43)

(Taghli-Lamallem et al., 2008)

eye disc | third instar larval stage (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

eye disc | third instar larval stage (with Dys^E6)

(Shcherbata et al., 2007)

eye photoreceptor cell (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

eye photoreceptor cell | third instar larval stage (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

eye photoreceptor cell | third instar larval stage (with Dys^E6)

(Shcherbata et al., 2007)

indirect flight muscle cell (with Df(3R)Delta-X43)

(Kucherenko et al., 2008)

indirect flight muscle cell | conditional (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

lamina plexus | third instar larval stage (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

lamina plexus | third instar larval stage (with Dys^E6)

(Shcherbata et al., 2007)

oocyte (with Df(3R)Delta-X43)

(Shcherbata et al., 2007)

Detailed Description

Statement

Reference

Dys^8-2/Df(3R)Dl-X43 mutant flies exhibit mild age-dependent indirect flight muscle degeneration.

(Kucherenko et al., 2008)

With age, Dys^8-2/Df(3R)Dl-X43 flies exhibit shorter diastolic

intervals, resulting in increased heart rates and fewer asystoles, and produce a more regular heartbeat than wild-type controls.

Throughout most of their adult life, Dys^8-2/Df(3R)Dl-X43 flies have a significantly wider diastolic and systolic diameter, accompanied (from 3 weeks onwards) by a reduced fractional shortening compared to controls.

(Taghli-Lamallem et al., 2008)

Dys^8-2/Df(3R)Dl-X43 mutant ovaries show oocyte polarity defects.

Dys^8-2/Df(3R)Dl-X43 mutant flies show comparable climbing ability at the beginning of adult life to control flies. However, the ability of these animals to climb declines significantly faster over time compared to controls. Animals exhibit mostly normal muscle architecture at 3 days after eclosion. By 12 days, age-dependent muscle degeneration is observed including loss of muscle fibre organisation, vacuolisation and the absence of some muscles.

Retinal photoreceptor cells are not elongated in Dys^8-2/Df(3R)Dl-X43 mutant eyes. In third instar larval eye discs, neuronal projections from photoreceptor neurons to the brain optic lobes are disturbed. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly.

Dys^8-2/Dys^E6 mutant third instar larval eye discs develop aberrant neuronal projections from photoreceptor neurons to the brain optic lobes. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly. This axon projection phenotype not observed in Dys^8-2/+ mutant discs.

(Shcherbata et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Dg³²³, Dys[+]/Dys^8-2 has abnormal cell polarity phenotype

(Shcherbata et al., 2007)

Dg³²³, Dys[+]/Dys^8-2 has abnormal neuroanatomy | third instar larval stage phenotype

(Shcherbata et al., 2007)

Phenotype Manifest In

Other

Statement

Reference

Dg³²³, Dys[+]/Dys^8-2 has oocyte phenotype

(Shcherbata et al., 2007)

Dg³²³, Dys[+]/Dys^8-2 has eye disc | third instar larval stage phenotype

(Shcherbata et al., 2007)

Dg³²³, Dys[+]/Dys^8-2 has eye photoreceptor cell | third instar larval stage phenotype

(Shcherbata et al., 2007)

Dg³²³, Dys[+]/Dys^8-2 has lamina plexus | third instar larval stage phenotype

(Shcherbata et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Dg³²³/+; Dys^8-2/+ double heterozygous oocytes show significant polarity defects indistinguishable from Dg³²³/Dg³²³ or Dys^8-2/Df(3R)Dl-X43 mutants.

Dg³²³/+; Dys^8-2/+ double heterozygous mutant third instar larval eye discs show disrupted neuronal projections from photoreceptor neurons to the brain optic lobes, showing breaks in the lamina surface.

dock¹³⁴²¹/+; Dys^8-2/+ or dock⁰⁴⁷²³/+; Dys^8-2/+ double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

InR³⁴/Dys^8-2 or Dys^8-2/InR^ex52.1 double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

(Shcherbata et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dys8-2

(Kucherenko et al., 2008)

Dys^8-2

(Shcherbata et al., 2007)

dys^8-2

(Taghli-Lamallem et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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