FB2024_03 , released June 25, 2024
Allele: Dmel\Diap1PRAP.UAS.Venus
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General Information
Symbol
Dmel\Diap1PRAP.UAS.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0244059
Feature type
allele
Associated gene
Dmel\Diap1
Associated Insertion(s)
Carried in Construct
P{UAS-DIAP1.PRAP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Koto et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-DIAP1.PRAP}
Cytology
Description

Amino acid replacement: N117K.

(Koto et al., 2009)

UASt regulatory sequences drive expression of an in vivo reporter of Diap1 protein turnover. The reporter consists of Diap1 coding sequence that is tagged with the Venus fluorescent protein at its C-terminus. In addition, to ensure that the protein does not interfere with caspase-dependent cell death signaling when it is expressed, it contains containing two point mutations (amino acid replacements N117K and H283Y) which have been shown to block binding to activated Drice and Dcp-1 and impair binding to pro-Dronc respectively.

(Koto et al., 2009)

Amino acid replacement: H283Y.

(Koto et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Diap1
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Diap1PRAP.UAS.Venus, Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF, rprUAS.cZa

      (Koto et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of thN117K.H283Y.Scer\UAS.T:Avic\GFP-YFP.Venus in the developing eye under the control of Scer\GAL4GMR.PF fails to suppress the 'small-eye' phenotype found in rprScer\UAS.cZa; Scer\GAL4GMR.PF flies.

      (Koto et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Diap1PRAP.UAS.Venus/Scer\GAL4sca-109-68 rescues Diap1RNAi.UAS.cOa

      (Koto et al., 2009)
      Comments

      The loss-of-bristle phentoype observed in thdsRNA.cOa.Scer\UAS-Scer\GAL4sca-109-68 flies is suppressed though expression of thN117K.H283Y.Scer\UAS.T:Avic\GFP-YFP.Venus.

      (Koto et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Diap1N117K.H283Y.Scer\UAS.T:Avic\GFP-YFP.Venus
      Diap1PRAP.UAS.Venus
      thN117K.H283Y.Scer\UAS.T:Avic\GFP-YFP.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (2)