FlyBase Allele Report: Dmel\Nct[ST3]

General Information

Symbol

Dmel\Nct^ST3

Species

D. melanogaster

Name

FlyBase ID

FBal0243772

Feature type

allele

Associated gene

Dmel\Nct

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Zhang et al., 2005)

Mutagen

ethyl methanesulfonate

(Zhang et al., 2005)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Zhang et al., 2005)

Amino acid replacement: W156term.

(Zhang et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:24,852,292..24,852,292 [+]

Nucleotide change:

G24852292A

Reported nucleotide change:

G?A

Amino acid change:

W156term | nct-PA; W156term | nct-PC; W156term | nct-PD; W156term | nct-PE

Reported amino acid change:

W156term

Comment:

TGG to TAG nonsense mutationi in codon W156.

(Zhang et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Nct^ST1)

(Zhang et al., 2005)

increased cell number (with Nct^ST1)

(Zhang et al., 2005)

lethal - all die before end of pupal stage

(Zhang et al., 2005)

lethal - all die before end of pupal stage (with Nct^ST1)

(Zhang et al., 2005)

some die during pupal stage

(Zhang et al., 2005)

some die during pupal stage (with Nct^ST1)

(Zhang et al., 2005)

Phenotype Manifest In

antennal disc | wandering third instar larval stage (with Nct^ST1)

(Zhang et al., 2005)

external sensory organ precursor cell | increased number | wandering third instar larval stage (with Nct^ST1)

(Zhang et al., 2005)

eye disc | somatic clone | wandering third instar larval stage

(Zhang et al., 2005)

eye disc | wandering third instar larval stage (with Nct^ST1)

(Zhang et al., 2005)

eye photoreceptor cell | somatic clone | increased number | wandering third instar larval stage

(Zhang et al., 2005)

eye photoreceptor cell | somatic clone | wandering third instar larval stage

(Zhang et al., 2005)

eye photoreceptor cell | wandering third instar larval stage (with Nct^ST1)

(Zhang et al., 2005)

leg disc | wandering third instar larval stage (with Nct^ST1)

(Zhang et al., 2005)

photoreceptor cell R8 | somatic clone | increased number | wandering third instar larval stage

(Zhang et al., 2005)

Detailed Description

Statement

Reference

Antennal discs from nct^ST1/nct^ST3 late third instar larvae display supernumerary sensory organ precursors. In the eye disc, a lack of photoreceptor neuron formation is observed, and the disc is reduced in size compared to wild-type. Mutant leg discs display supernumerary sensory organ precursors and neurons.

Homozygous nct^ST3 large mutant eye disc clones (generated in a Minute/+ background) display very few neurons, and an overall reduction in disc size is observed. On the contrary, small nct^ST3 clones (generated in a wild-type background) display supernumerary neurons, and extra R8 neurons are present.

(Zhang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

N^int.U.UAS, Nct^ST3/Nct^ST1, Scer\GAL4^dpp.blk1 has increased cell number phenotype

(Zhang et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nct^ST3/Nct^ST1 has eye photoreceptor cell | wandering third instar larval stage phenotype, suppressible by N^int.U.UAS/Scer\GAL4^dpp.blk1

(Zhang et al., 2005)

Nct^ST3/Nct^ST1 has eye disc | wandering third instar larval stage phenotype, suppressible by N^int.U.UAS/Scer\GAL4^dpp.blk1

(Zhang et al., 2005)

Suppressor of

Statement

Reference

Nct^ST3/Nct^ST1 is a suppressor of ommatidial precursor cluster | wandering third instar larval stage phenotype of N^int.U.UAS, Scer\GAL4^dpp.blk1

(Zhang et al., 2005)

Other

Statement

Reference

N^int.U.UAS, Nct^ST3/Nct^ST1, Scer\GAL4^dpp.blk1 has eye disc | wandering third instar larval stage phenotype

(Zhang et al., 2005)

N^int.U.UAS, Nct^ST3/Nct^ST1, Scer\GAL4^dpp.blk1 has photoreceptor cell R8 | increased number | wandering third instar larval stage phenotype

(Zhang et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 in a nct^ST1/nct^ST3 mutant background results in larger third instar eye discs compared to wild-type. The missing neuronal clusters observed in N^{int.U.Scer\UAS}; Scer\GAL4^dpp.blk1 eye discs are restored in a nct^ST1/nct^ST3 mutant background. However, R8 cells are not as regularly arrayed as in wild-type or N^{int.U.Scer\UAS}; Scer\GAL4^dpp.blk1 eye discs. An increase R8 cell number is also observed, including more than one R8 cell per cluster.

(Zhang et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Nct^ST1

(Zhang et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nct^ST3

nct^ST3

(Zhang et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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