Amino acid replacement: Q24term.
C15815990T
Q24term | fwe-PA; Q24term | fwe-PB; Q24term | fwe-PC
Q24term
Nucleotide substitution inferred from reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator.
lethal | adult stage (with fweEY08496)
lethal | pupal stage (with fweEY08496)
NMJ bouton | heat sensitive (with fweDB25)
NMJ bouton | larval stage (with fweDB25)
synaptic vesicle | larval stage (with fweDB25)
Synaptic boutons of fweDB56/fweDB25 mutant larval neuromuscular junctions exhibit impaired Clathrin-mediated endocytosis (but not exocytosis), impaired activity-dependent bulk endocytosis, a significant decrease in bulk cisternae induction after high K[+] treatment, and a significant decrease in the number of synaptic vesicles as well as increased average vesicle size, as compared to controls, and these larvae also exhibit early larval lethality.
The NMJs of fweDB25/fweDB56 mutant larvae raised at 25[o]C have smaller and more numerous boutons compared to controls, often arrayed in bunches, and are compromised in synaptic transmission. Rearing fweDB25/fweDB56 larvae at 18°C significantly reduces synaptic bouton number and arrangement to resemble wild-type.
flowerDB56 eye somatic clone mutants are morphologically normal. However, they display impaired 'on-off' transients in electroretinograms and slightly reduced depolarizations. The synaptic patterns of R7 and R8 photoreceptor subtypes of flowerDB56 mutants and wild-type are indistinguishable. The number of synaptic vesicles is reduced in flowerDB56 mutants compared to controls. They also exhibit aberrant sizes and a varying number of capitate projections.
flowerDB56 mutants display numerous extra boutons which are often small, clustered, and flowery in nature. However, the total number of active zones and the total NMJ surface area is similar to controls.
Evoked EJPs are 0.2Hz in 0.5mm or 1.0mM amplitudes in flowerDB56 mutants are similar to controls. At 10Hz in 0.5mM '[Ca[2+]'], wild-type controls display facilitation, whereas mutants display a rundown. Post-tetanic potentiation is severely impaired in flowerDB56 mutants and the EJPs recover slowly.Increased extracellular Ca[2+] concentrations can partially rescue the endocytic defects.
There is an increase in the quantal size and a decrease in the frequency of mEJPs in flowerDB56 mutants. The junctional quantal content estimated from dividing EJP by mEJP in flowerDB56 mutants is decreased by approximately 29%. flowerDB56 mutants exhibit a 41% reduction in FM1-43 dye uptake as compared to controls.
flowerDB56 mutant boutons display a severe reduction in number of synaptic vesicles (SV). SVs are also more heterogeneous in size, and their diameter is increased when compared to control boutons. There are significantly more endocytic intermediates, such as omega structures and collared pits, after stimulation.
There is a significant decrease in resting intracellular Ca[2+] levels in flowerDB56 mutants compared to controls.
fweDB56/fweDB25 has abnormal neuroanatomy | larval stage phenotype, suppressible by Mmus\Cacfd1UAS.2.Tag:FLAG,Tag:HA/Scer\GAL4nSyb.PS
fweDB56/fweDB25 has lethal - all die before end of larval stage phenotype, suppressible by Mmus\Cacfd1UAS.2.Tag:FLAG,Tag:HA/Scer\GAL4nSyb.PS
fweDB56/fweDB25 has NMJ bouton | larval stage phenotype, suppressible by Mmus\Cacfd1UAS.2.Tag:FLAG,Tag:HA/Scer\GAL4nSyb.PS
fweDB56/fweDB25 has synaptic vesicle | larval stage phenotype, suppressible by Mmus\Cacfd1UAS.2.Tag:FLAG,Tag:HA/Scer\GAL4nSyb.PS
shi1/flowerDB56 mutants are synthetic lethal.
Expression of Mmus\Cacfd1Scer\UAS.2.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS rescues the Clathrin-mediated endocytosis defects, the activity-dependent bulk endocytosis defects, and the lowered synaptic vesicle numbers seen in larval neuromuscular junctions of fweDB25/fweDB56 mutants, and also rescues the early larval lethality observed in these mutants.
fweDB56/fweDB25 is rescued by fweRB.UAS.Tag:HA.Tag:FLAG/Scer\GAL4nSyb.PS
fweDB56/fweDB25 is rescued by Scer\GAL4elav.PU/fweRB.UAS.Tag:HA.Tag:FLAG
fweDB56 is rescued by Scer\GAL4elav-C155/fweRA.UAS.Tag:HA
fweDB56 is rescued by fweRA.UAS.Tag:HA/Scer\GAL4GMR.PF
fweDB56 is rescued by Scer\GAL4elav-C155/fweRB.UAS.Tag:HA.Tag:FLAG
fweDB56 is rescued by fweRB.UAS.Tag:HA.Tag:FLAG/Scer\GAL4GMR.PF
fweDB56 is rescued by fwe+8.2.RA.Tag:FLAG.RB.Tag:HA.RC.Tag:MYC
fweDB56 is rescued by fwe+8.2.RA.YFP.RB.GFP.RC.mRFP(Unk)
fweDB56/fweDB25 is rescued by fwe+8.2.RA.Tag:FLAG.RB.Tag:HA.RC.Tag:MYC
fweDB56/fweDB25 is rescued by fwe+8.2.RA.YFP.RB.GFP.RC.mRFP(Unk)
fweDB56/fweDB25 is partially rescued by fweRB.E79Q.UAS.Tag:HA.Tag:FLAG/Scer\GAL4nSyb.PS
fweDB56 is not rescued by Scer\GAL4elav-C155/fweRC.UAS.Tag:FLAG
Expression of fweRB.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4nSyb.PS or Scer\GAL4elav.PU completely rescues the Clathrin-mediated endocytosis defects, and when expressed under the control of Scer\GAL4nSyb.PS, rescues the activity-dependent bulk endocytosis and lowered synaptic vesicle numbers and the increased vesicle size seen in larval neuromuscular junctions of fweDB25/fweDB56 mutants.
Expression of fweRB.E79Q.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4nSyb.PS completely rescues the Clathrin-mediated endocytosis defects, the activity-dependent bulk endocytosis defects, the lowered synaptic vesicle numbers, and the increased vesicle size, but does not rescue the decreased bulk cisternae, seen in larval neuromuscular junctions of fweDB25/fweDB56 mutants.