FlyBase Allele Report: Dmel\fwe[DB56]

General Information

Symbol

Dmel\fwe^DB56

Species

D. melanogaster

Name

FlyBase ID

FBal0241889

Feature type

allele

Associated gene

Dmel\fwe

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yao et al., 2009)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q24term.

(Yao et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:15,815,990..15,815,990 [-]

Nucleotide change:

C15815990T

Amino acid change:

Q24term | fwe-PA; Q24term | fwe-PB; Q24term | fwe-PC

Reported amino acid change:

Q24term

Comment:

Nucleotide substitution inferred from reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator.

(Yao et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | heat sensitive (with fwe^DB25)

(Baxter et al., 2014)

abnormal neuroanatomy | larval stage (with fwe^DB25)

(Yao et al., 2017)

increased cell death | dominant | larval stage

(Merino et al., 2022)

lethal | adult stage (with fwe^EY08496)

(Yao et al., 2009)

lethal | pupal stage (with fwe^EY08496)

(Yao et al., 2009)

lethal - all die before end of first instar larval stage

(Yao et al., 2009)

lethal - all die before end of first instar larval stage (with Df(3L)X-21.2)

(Yao et al., 2009)

lethal - all die before end of larval stage (with fwe^DB25)

(Yao et al., 2017)

lethal - all die before end of pupal stage (with fwe^DB25)

(Yao et al., 2009)

some die during first instar larval stage

(Yao et al., 2009)

some die during first instar larval stage (with Df(3L)X-21.2)

(Yao et al., 2009)

some die during pupal stage (with fwe^DB25)

(Yao et al., 2009)

Phenotype Manifest In

NMJ bouton | heat sensitive (with fwe^DB25)

(Baxter et al., 2014)

NMJ bouton | larval stage (with fwe^DB25)

(Yao et al., 2017)

synaptic vesicle | larval stage (with fwe^DB25)

(Yao et al., 2017)

wing disc | larval stage

(Merino et al., 2022)

Detailed Description

Statement

Reference

Synaptic boutons of fwe^DB56/fwe^DB25 mutant larval neuromuscular junctions exhibit impaired Clathrin-mediated endocytosis (but not exocytosis), impaired activity-dependent bulk endocytosis, a significant decrease in bulk cisternae induction after high K[+] treatment, and a significant decrease in the number of synaptic vesicles as well as increased average vesicle size, as compared to controls, and these larvae also exhibit early larval lethality.

(Yao et al., 2017)

The NMJs of fwe^DB25/fwe^DB56 mutant larvae raised at 25[o]C have smaller and more numerous boutons compared to controls, often arrayed in bunches, and are compromised in synaptic transmission. Rearing fwe^DB25/fwe^DB56 larvae at 18°C significantly reduces synaptic bouton number and arrangement to resemble wild-type.

(Baxter et al., 2014)

flower^DB56 eye somatic clone mutants are morphologically normal. However, they display impaired 'on-off' transients in electroretinograms and slightly reduced depolarizations. The synaptic patterns of R7 and R8 photoreceptor subtypes of flower^DB56 mutants and wild-type are indistinguishable. The number of synaptic vesicles is reduced in flower^DB56 mutants compared to controls. They also exhibit aberrant sizes and a varying number of capitate projections.

flower^DB56 mutants display numerous extra boutons which are often small, clustered, and flowery in nature. However, the total number of active zones and the total NMJ surface area is similar to controls.

Evoked EJPs are 0.2Hz in 0.5mm or 1.0mM amplitudes in flower^DB56 mutants are similar to controls. At 10Hz in 0.5mM '[Ca[2+]'], wild-type controls display facilitation, whereas mutants display a rundown. Post-tetanic potentiation is severely impaired in flower^DB56 mutants and the EJPs recover slowly.Increased extracellular Ca[2+] concentrations can partially rescue the endocytic defects.

There is an increase in the quantal size and a decrease in the frequency of mEJPs in flower^DB56 mutants. The junctional quantal content estimated from dividing EJP by mEJP in flower^DB56 mutants is decreased by approximately 29%. flower^DB56 mutants exhibit a 41% reduction in FM1-43 dye uptake as compared to controls.

flower^DB56 mutant boutons display a severe reduction in number of synaptic vesicles (SV). SVs are also more heterogeneous in size, and their diameter is increased when compared to control boutons. There are significantly more endocytic intermediates, such as omega structures and collared pits, after stimulation.

There is a significant decrease in resting intracellular Ca[2+] levels in flower^DB56 mutants compared to controls.

(Yao et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

fwe^DB56/fwe^DB25 has abnormal neuroanatomy | larval stage phenotype, suppressible by Mmus\Cacfd1^{UAS.2.Tag:FLAG,Tag:HA}/Scer\GAL4^nSyb.PS

(Yao et al., 2017)

fwe^DB56/fwe^DB25 has lethal - all die before end of larval stage phenotype, suppressible by Mmus\Cacfd1^{UAS.2.Tag:FLAG,Tag:HA}/Scer\GAL4^nSyb.PS

(Yao et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

fwe^DB56/fwe^DB25 has NMJ bouton | larval stage phenotype, suppressible by Mmus\Cacfd1^{UAS.2.Tag:FLAG,Tag:HA}/Scer\GAL4^nSyb.PS

(Yao et al., 2017)

fwe^DB56/fwe^DB25 has synaptic vesicle | larval stage phenotype, suppressible by Mmus\Cacfd1^{UAS.2.Tag:FLAG,Tag:HA}/Scer\GAL4^nSyb.PS

(Yao et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

shi¹/flower^DB56 mutants are synthetic lethal.

(Yao et al., 2009)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Cacfd1^{Scer\UAS.2.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^nSyb.PS rescues the Clathrin-mediated endocytosis defects, the activity-dependent bulk endocytosis defects, and the lowered synaptic vesicle numbers seen in larval neuromuscular junctions of fwe^DB25/fwe^DB56 mutants, and also rescues the early larval lethality observed in these mutants.

(Yao et al., 2017)

Complementation and Rescue Data

Fails to complement

fwe^EY08496

(Yao et al., 2009)

Rescued by

fwe^DB56/fwe^DB25 is rescued by fwe^{RB.UAS.Tag:HA.Tag:FLAG}/Scer\GAL4^nSyb.PS

(Yao et al., 2017)

fwe^DB56/fwe^DB25 is rescued by Scer\GAL4^elav.PU/fwe^{RB.UAS.Tag:HA.Tag:FLAG}

(Yao et al., 2017)

fwe^DB56 is rescued by Scer\GAL4^elav-C155/fwe^{RA.UAS.Tag:HA}

(Yao et al., 2009)

fwe^DB56 is rescued by fwe^{RA.UAS.Tag:HA}/Scer\GAL4^GMR.PF

(Yao et al., 2009)

fwe^DB56 is rescued by Scer\GAL4^elav-C155/fwe^{RB.UAS.Tag:HA.Tag:FLAG}

(Yao et al., 2009)

fwe^DB56 is rescued by fwe^{RB.UAS.Tag:HA.Tag:FLAG}/Scer\GAL4^GMR.PF

(Yao et al., 2009)

fwe^DB56 is rescued by fwe^{+8.2.RA.Tag:FLAG.RB.Tag:HA.RC.Tag:MYC}

(Yao et al., 2009)

fwe^DB56 is rescued by fwe^{+8.2.RA.YFP.RB.GFP.RC.mRFP(Unk)}

(Yao et al., 2009)

fwe^DB56/fwe^DB25 is rescued by fwe^{+8.2.RA.Tag:FLAG.RB.Tag:HA.RC.Tag:MYC}

(Yao et al., 2009)

fwe^DB56/fwe^DB25 is rescued by fwe^{+8.2.RA.YFP.RB.GFP.RC.mRFP(Unk)}

(Yao et al., 2009)

Partially rescued by

fwe^DB56/fwe^DB25 is partially rescued by fwe^{RB.E79Q.UAS.Tag:HA.Tag:FLAG}/Scer\GAL4^nSyb.PS

(Yao et al., 2017)

Not rescued by

fwe^DB56 is not rescued by Scer\GAL4^elav-C155/fwe^{RC.UAS.Tag:FLAG}

(Yao et al., 2009)

Comments

Expression of fwe^{RB.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} under the control of Scer\GAL4^nSyb.PS or Scer\GAL4^elav.PU completely rescues the Clathrin-mediated endocytosis defects, and when expressed under the control of Scer\GAL4^nSyb.PS, rescues the activity-dependent bulk endocytosis and lowered synaptic vesicle numbers and the increased vesicle size seen in larval neuromuscular junctions of fwe^DB25/fwe^DB56 mutants.

Expression of fwe^{RB.E79Q.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} under the control of Scer\GAL4^nSyb.PS completely rescues the Clathrin-mediated endocytosis defects, the activity-dependent bulk endocytosis defects, the lowered synaptic vesicle numbers, and the increased vesicle size, but does not rescue the decreased bulk cisternae, seen in larval neuromuscular junctions of fwe^DB25/fwe^DB56 mutants.

(Yao et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51610

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}80B fwe^DB56/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DB25

(Yao et al., 2009)

flower^DB56

fwe^DB56

(Merino et al., 2022, Li et al., 2020, Ozdowski et al., 2020, Yao et al., 2017, Baxter et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

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