FlyBase Allele Report: Dmel\Axud1[UAS.cGa]

General Information

Symbol

Dmel\Axud1^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Glavic

FlyBase ID

FBal0241583

Feature type

allele

Associated gene

Dmel\Axud1

Associated Insertion(s)

Carried in Construct

P{UAS-Axud1.G}

Key Links

Transgenic product class

cDNA

(Glavic et al., 2009)

wild_type

(Glavic et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Glavic et al., 2009)

wild_type

(Glavic et al., 2009)

Mutagen

in vitro construct

(Glavic et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Axud1.G}

Cytology

Description

UAS regulatory sequences drive expression of an Axud1 cDNA.

(Glavic et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Axud1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage, with Scer\GAL4^salm.EPv

(Glavic et al., 2009)

abnormal size, with Scer\GAL4^salm.EPv

(Glavic et al., 2009)

increased cell death, with Scer\GAL4^GMR.PFa

(Glavic et al., 2009)

visible, with Scer\GAL4^ey.PH

(Glavic et al., 2009)

visible, with Scer\GAL4^MD-638

(Glavic et al., 2009)

visible, with Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Glavic et al., 2009)

eye disc, with Scer\GAL4^GMR.PFa

(Glavic et al., 2009)

submarginal cell, with Scer\GAL4^salm.EPv

(Glavic et al., 2009)

wing blade, with Scer\GAL4^MD-638

(Glavic et al., 2009)

wing blade, with Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^salm-EPv-driven expression of Axud1^Scer\UAS.cGa in the central region of the wing (L2 to L4) produces a non-allometric reduction in size and the collapse of the L2-L3 intervein.

Eye size is reduced as a result of Scer\GAL4^ey.PH-driven expression of Axud1^Scer\UAS.cGa.

The number of cells in S-phase is severely reduced, while the number of mitotic cells is increased when Axud1^Scer\UAS.cGa is ectopically expressed with Scer\GAL4^salm-EPv in the larval wing imaginal disc.

Scer\GAL4^MD-638-driven expression of Axud1^Scer\UAS.cGa strongly impairs wing development.

Larval eye imaginal disc cells expressing Scer\GAL4^GMR.PFa-driven Scer\GAL4[GMR.PFa] enter apoptosis as result of mitotic blockage.

(Glavic et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has visible phenotype, enhanceable by puc^E69/puc[+], Scer\GAL4^salm.EPv

(Glavic et al., 2009)

NOT Enhanced by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has abnormal cell cycle phenotype, non-enhanceable by BacA\p35^UAS.cHa

(Glavic et al., 2009)

Suppressed by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has visible phenotype, suppressible by bsk^J27/bsk[+], Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^MD-638 has visible phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^MD-638

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has visible phenotype, suppressible by hep[+]/hep^r75, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

NOT suppressed by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has abnormal cell cycle phenotype, non-suppressible by BacA\p35^UAS.cHa

(Glavic et al., 2009)

Other

Statement

Reference

Axud1^UAS.cGa, BacA\p35^UAS.cHa, Scer\GAL4^salm.EPv has visible phenotype

(Glavic et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing blade phenotype, enhanceable by puc^E69/puc[+], Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing phenotype, enhanceable by puc^E69/puc[+], Scer\GAL4^salm.EPv

(Glavic et al., 2009)

NOT Enhanced by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing blade phenotype, non-enhanceable by BacA\p35^UAS.cHa

(Glavic et al., 2009)

Suppressed by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^MD-638 has wing blade phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^MD-638

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^MD-638 has wing phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^MD-638

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing blade phenotype, suppressible by hep[+]/hep^r75, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing blade phenotype, suppressible by bsk^J27/bsk[+], Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing phenotype, suppressible by hep[+]/hep^r75, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing phenotype, suppressible by bsk^J27/bsk[+], Scer\GAL4^salm.EPv

(Glavic et al., 2009)

NOT suppressed by

Statement

Reference

Axud1^UAS.cGa, Scer\GAL4^salm.EPv has wing blade phenotype, non-suppressible by BacA\p35^UAS.cHa

(Glavic et al., 2009)

Other

Statement

Reference

Axud1^UAS.cGa, BacA\p35^UAS.cHa, Scer\GAL4^salm.EPv has wing blade phenotype

(Glavic et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Axud1^Scer\UAS.cGa with Scer\GAL4^salm-EPv in a heterozygous puc^E69 background significantly enhances the reduction in wing size.

Expression of Axud1^Scer\UAS.cGa with Scer\GAL4^salm-EPv in a heterozygous hep^r75 background results in a partial suppression of the ectopic Axud1^Scer\UAS.cGa expression wing phenotype.

Expression of Axud1^Scer\UAS.cGa with Scer\GAL4^salm-EPv in a heterozygous bsk^J27 background results in a partial suppression of the ectopic Axud1^Scer\UAS.cGa expression wing phenotype.

The wing impairment resulting from the ectopic expression of Scer\GAL4^MD-638-driven Axud1^Scer\UAS.cGa is suppressed by the co-expression of cdc2^{Scer\UAS.T:Hsap\MYC}.

(Glavic et al., 2009)

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^salm-EPv-driven co-expression of BacA\p35^Scer\UAS.cHa with Axud1^Scer\UAS.cGa produces epithelial extrusion in the adult wing.

The cell cycle abnormalities of the larval wing imaginal disc cells resulting from the Scer\GAL4^salm-EPv-driven expression of Axud1^Scer\UAS.cGa are not modified by the co-expression of BacA\p35^Scer\UAS.cHa.

(Glavic et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Axud1^Scer\UAS.cGa

Axud1^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Glavic

Secondary FlyBase IDs

References (1)

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