FB2024_03 , released June 25, 2024
Allele: Zzzz\CAG63Q.UAS.Tag:HA
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General Information
Symbol
Zzzz\CAG63Q.UAS.Tag:HA
Species
a. artificial
Name
FlyBase ID
FBal0241468
Feature type
allele
Associated gene
Zzzz\CAG
Associated Insertion(s)
Carried in Construct
P{UAS-Zzzz\CAG.63Q}
Also Known As
UAS-63Q
Transgenic product class
trinucleotide_repeat_expansion
(Kazemi-Esfarjani and Benzer, 2002)
Mutagen
Nature of the Allele
Transgenic product class
trinucleotide_repeat_expansion
(Kazemi-Esfarjani and Benzer, 2002)
Mutagen
in vitro construct
(Kazemi-Esfarjani and Benzer, 2002)
Progenitor genotype
Carried in construct
P{UAS-Zzzz\CAG.63Q}
Cytology
Description

UAS regulatory sequences drive expression of 63 Zzzz\CAG poly(Q) repeats, fused at the C-terminal end with Tag:HA.

(Kazemi-Esfarjani and Benzer, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Zzzz\CAG
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU results in a rough eye defect.

      (Chen et al., 2012)

      Females expressing Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a maintain their vitality and numbers up to 8 days posteclosion, after which they begin a decline in both vitality and numbers that inevitably decimates the whole population by day 20.

      Retinas appear disorganised in 5 week old flies expressing Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ninaE.PD.

      (Fayazi et al., 2006)

      Expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 in the developing eye under the control of Scer\GAL4GMR.PF results in severely collapsed eyes that lack pigmentation. An accumulation of poly(Q) clumps is detected in mutant eyes.

      Expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 in the developing nervous system under the control of Scer\GAL4Appl.G1a results in pre-adult lethality in many cases, with only one transgenic line giving rise to female adults.

      Flies carrying Scer\GAL4Appl.G1a plus a heterozygous insertion of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 become progressively lethargic by day 12, with almost all the flies dying before day 20. Flies first become unable to climb the walls of the plastic vial, and die within a few hours. Aggregates form in the neuronal cell bodies of the cortices surrounding the neuropils of the brain and the thoracic ganglion in 1-day old adults. No signs of neuronal degeneration are visible in either the brain or the thoracic ganglion, even in the last flies surviving up to 20 days.

      (Kazemi-Esfarjani and Benzer, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The rough eye defect caused by expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is not rescued by co-expression of either Hsp27Scer\UAS.T:Ivir\HA1 or Atg7Scer\UAS.cCa.

      The rough eye defect caused by expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is not enhanced by co-expression of either Hsp27GD11327 or Atg7GD11671.

      (Chen et al., 2012)

      Co-expression of mrjScer\UAS.T:Zzzz\FLAG significantly increases the survival of females expressing Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a, such that the decline the population after eclosion is delayed.

      Co-expression of DnaJ-1Scer\UAS.cKa suppresses the short-lived phenotype of females expressing Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a.

      (Fayazi et al., 2006)

      Expression of MlfScer\UAS.cKa under the control of Scer\GAL4Appl.G1a has a protective effect against Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 toxicity. At day 20, 55% of flies expressing Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 along with MlfScer\UAS.cKa (both under the control of Scer\GAL4Appl.G1a) are still active, while those expressing Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 alone are dead.

      Co-expression of DrefCR1.Scer\UAS with Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 in the eye under the control of Scer\GAL4GMR.PF does not suppress the eye degeneration observed upon expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 alone.

      Co-expression of zenScer\UAS.cKa with Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 in the eye under the control of Scer\GAL4GMR.PF does not suppress the eye degeneration observed upon expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 alone.

      (Kazemi-Esfarjani and Benzer, 2002)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1
      Zzzz\CAG63Q.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (4)