FlyBase Allele Report: Dmel\Smn[RNAi.FL.UAS.WIZ]

General Information

Symbol

Dmel\Smn^{RNAi.FL.UAS.WIZ}

Species

D. melanogaster

Name

FlyBase ID

FBal0241135

Feature type

allele

Associated gene

Dmel\Smn

Associated Insertion(s)

Carried in Construct

P{UAS-Smn.RNAi.FL}

Also Known As

UAS-Smn-RNAi^FL26B

Key Links

Transgenic product class

RNAi_reagent

(Chang et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Chang et al., 2008)

Mutagen

in vitro construct

(Chang et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Smn.RNAi.FL}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of sequences corresponding to the entire Smn cDNA.

(Chang et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Smn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of childhood spinal muscular atrophy

CEA

(Chang et al., 2008)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

NMJ bouton, with Scer\GAL4^Act.PU

(Chang et al., 2008)

NMJ bouton, with Scer\GAL4^elav.PLu

(Chang et al., 2008)

NMJ bouton, with Scer\GAL4^how-24B

(Chang et al., 2008)

NMJ bouton | third instar larval stage, with Scer\GAL4^Tub.PU

(Sen et al., 2013)

Detailed Description

Statement

Reference

Expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} driven by Scer\GAL4^Mef2.PR does not significantly affect flight ability in flies.

(Borg et al., 2015)

Expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU results in a fully penetrant pupal lethality, with approximately 40% of the pupae reaching a pigmented developmental stage.

Expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU in a Smn^X7 background results in a fully penetrant pupal lethality, with none of the pupae reaching a pigmented developmental stage.

Larvae expressing Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU show a reduction in bouton number at the neuromuscular junction.

(Sen et al., 2013)

Expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of one of Scer\GAL4^Act, Scer\GAL4^elav.PLu or Scer\GAL4^how-24B results in a reduction in bouton number per muscle area at the neuromuscular junction of third instar larvae compared to wild type.

(Chang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by PBac{PB}CG6000^c03607/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has lethal - all die during pupal stage phenotype, enhanceable by CG13775^f05549

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has lethal - all die during pupal stage phenotype, enhanceable by Atf6^c05057

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by shrb^d02738/shrb[+]

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Sod[+]/Sod1^c02456

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by PBac{RB}e01468/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by P{XP}d00200/+

(Sen et al., 2013)

Scer\GAL4^αTub84B.PL, Smn^{RNAi.FL.UAS.WIZ} has some die during P-stage phenotype, enhanceable by btl^f02864

(Sen et al., 2011)

Suppressed by

Statement

Reference

+:, Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by PBac{WH}sel^f04257/PBac{WH}PAPLA1^f04257/+/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by eIF3m^c05501/Tango7[+]

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by 14-3-3ε^c06705/14-3-3epsilon[+]

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by PBac{RB}e03932/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has lethal - all die during pupal stage phenotype, suppressible by wit^d02492

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has lethal - all die during pupal stage phenotype, suppressible by Eip75B^d09801

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by P{XP}d04314/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by PBac{WH}CG7091^f05023/+

(Sen et al., 2013)

Other

Statement

Reference

Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR, Smn^{RNAi.FL.UAS.WIZ} has lethal phenotype

(Borg et al., 2015)

Scer\GAL4^αTub84B.PL, Smn^{RNAi.FL.UAS.WIZ}, btl^λ.UAS has lethal phenotype

(Sen et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, enhanceable by PBac{PB}CG6000^c03607/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, enhanceable by shrb^d02738/shrb[+]

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, enhanceable by Sod[+]/Sod1^c02456

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, enhanceable by PBac{RB}e01468/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, enhanceable by P{XP}d00200/+

(Sen et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, suppressible by P{XP}d04314/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, suppressible by PBac{WH}CG7091^f05023/+

(Sen et al., 2013)

+:, Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, suppressible by PBac{WH}sel^f04257/PBac{WH}PAPLA1^f04257/+/+

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, suppressible by eIF3m^c05501/Tango7[+]

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, suppressible by 14-3-3ε^c06705/14-3-3epsilon[+]

(Sen et al., 2013)

Scer\GAL4^Tub.PU, Smn^{RNAi.FL.UAS.WIZ} has NMJ bouton | third instar larval stage phenotype, suppressible by PBac{RB}e03932/+

(Sen et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gem3^ΔN.Scer\UAS and Smn^{dsRNA.FL.Scer\UAS.WIZ} driven by Scer\GAL4^Mef2.PR results in lethality.

(Borg et al., 2015)

wit^d02492 suppresses the pupal lethality caused by expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU : the proportion of pupae reaching a pigmented stage is increased.

Eip75B^d09801 suppresses the pupal lethality caused by expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU : the proportion of pupae reaching a pigmented stage is increased.

CG13775^f05549 enhances the pupal lethality caused by expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU : the proportion of pupae reaching a pigmented stage is reduced.

Atf6^c05057 enhances the pupal lethality caused by expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU : the proportion of pupae reaching a pigmented stage is reduced.

Heterozygosity for either PBac{PB}CG6000^c03607, shrb^d02738, Sod^c02456, PBac{RB}e01468 or P{XP}d00200 enhances the reduction in bouton number at the neuromuscular junction seen in third instar larvae expressing Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU.

Heterozygosity for either P{XP}d04314, PBac{WH}CG7091^f05023, Tango7^c05501, 14-3-3ε^c06705 or PBac{RB}e03932 suppresses the reduction in bouton number at the neuromuscular junction seen in third instar larvae expressing Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU.

Heterozygosity for the "f04257" line (which carries the insertions PBac{WH}sel^f04257 and PBac{WH}CG8552^f04257 suppresses the reduction in bouton number at the neuromuscular junction seen in third instar larvae expressing Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU.

(Sen et al., 2013)

Co-expression of btl^{Scer\UAS.T:λ\cI-DD} leads to complete pupal lethality in files with expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} driven by Scer\GAL4^αTub84B.PL. Presence of btl^f02864 increases the amount of pupal lethality seen in Scer\GAL4^αTub84B.PL>Smn^{dsRNA.FL.Scer\UAS.WIZ} flies.

(Sen et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Smn^{RNAi.FL.UAS.WIZ} is rescued by Scer\GAL4^Tub.PU/Smn^UAS.GFP

(Sen et al., 2013)

Comments

Co-expression of Smn^{Scer\UAS.T:Avic\GFP} rescues the pupal lethality caused by expression of Smn^{dsRNA.FL.Scer\UAS.WIZ} under the control of Scer\GAL4^tub.PU.

(Sen et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Smn^{RNAi.FL.UAS.WIZ}

Smn^{dsRNA.FL.Scer\UAS.WIZ}

Smn^{dsRNA.FL.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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