FlyBase Allele Report: Dmel\tsh[JF02856]

General Information

Symbol

Dmel\tsh^JF02856

Species

D. melanogaster

Name

FlyBase ID

FBal0240826

Feature type

allele

Associated gene

Dmel\tsh

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02856}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02856}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02856

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

short lived, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

some die during pupal stage, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

visible, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Phenotype Manifest In

adult abdomen, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

adult Malpighian tubule, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

adult Malpighian tubule Type II cell, with Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Detailed Description

Statement

Reference

Expression of tsh^JF02856 in the fat body under the control of Scer\GAL4^DcG results in significant hyperglycaemia.

(Ugrankar et al., 2015)

Only 12% of animals expressing tsh^JF02856 under the control of Scer\GAL4^tsh-c724 emerge as adults, and the adults ares short lived compared to controls. 1 week old adults have grossly distended abdomens due to fluid retention.

Malpighian tubules from adults expressing tsh^JF02856 under the control of Scer\GAL4^tsh-c724 show significantly lower basal secretion rates than control tubules. Addition of cAMP increases the secretion rate in the mutant tubules, however the peak rate is significantly lower than control tubules. Addition of Leucokinin in addition to cAMP has no further stimulatory effect on the mutant tubules, in contrast to controls which show a further doubling of the secretion rate.

Uric acid crystals accumulate in the lumen of the Malpighian tubules in animals expressing tsh^JF02856 under the control of Scer\GAL4^tsh-c724.

The number of stellate cells in the anterior and posterior Malpighian tubules is normal in adults expressing tsh^JF02856 under the control of Scer\GAL4^tsh-c724 and the spacing of stellate cells in these tubules is indistinguishable from wild type. The morphogenetic transformations that usually occur in the stellate and bar-shaped cells of the Malpighian tubules during pupation do not occur in the mutant animals; the cells remain cuboidal both in the main and initial/transitional regions of the adult tubules. The mutant adult stellate cells have less than half the surface area of stellate cells in wild-type tubules.

(Denholm et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^tsh-c724, tsh^JF02856 has partially lethal phenotype, suppressible | partially by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Scer\GAL4^tsh-c724, tsh^JF02856 has visible phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^tsh-c724, tsh^JF02856 has adult Malpighian tubule Type II cell phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Scer\GAL4^tsh-c724, tsh^JF02856 has adult abdomen phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^tsh-c724

(Denholm et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tio^Scer\UAS.cLa restores stellate cell morphology is restored in adults expressing tsh^JF02856 under the control of Scer\GAL4^tsh-c724. In addition, adult viability is partially rescued (22% of adults eclose) and the adults that emerge do not have bloated abdomens.

(Denholm et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28022

y¹ v¹; P{TRiP.JF02856}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tsh^JF02856

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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