FlyBase Allele Report: Dmel\Ubqn[KK100857]

General Information

Symbol

Dmel\Ubqn^KK100857

Species

D. melanogaster

Name

FlyBase ID

FBal0230953

Feature type

allele

Associated gene

Dmel\Ubqn

Associated Insertion(s)

Carried in Construct

P{KK100857}

Also Known As

UAS-dUbqnIR_107-494

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100857}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100857

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Jantrapirom et al., 2018)

model of frontotemporal dementia

CEA

(Jantrapirom et al., 2018)

model of neurodegenerative disease

CEA

(Jantrapirom et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^HM05194

(Jantrapirom et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Scer\GAL4^elav.PU

(Jantrapirom et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018, Jantrapirom et al., 2018)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Toll-6-D42

(Jantrapirom et al., 2018)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018, Jantrapirom et al., 2018)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Jantrapirom et al., 2018)

abnormal memory | adult stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

abnormal size | third instar larval stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

abnormal smell perception | adult stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

decreased rate of movement | adult stage, with Scer\GAL4^elav.PU

(Jantrapirom et al., 2020)

lethal - all die before end of P-stage, with Scer\GAL4^da.G32

(Lipinszki et al., 2011)

short lived, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

some die during pharate adult stage, with Scer\GAL4^da.G32

(Lipinszki et al., 2011)

Phenotype Manifest In

embryonic/larval brain | third instar larval stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^elav.PU

(Jantrapirom et al., 2020)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Jantrapirom et al., 2018)

eye, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

eye, with Scer\GAL4^GMR.PS

(Jantrapirom et al., 2018)

mushroom body | third instar larval stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

NMJ bouton | decreased number | larval stage, with Scer\GAL4^elav.PU

(Jantrapirom et al., 2020)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav.PLu

(Jantrapirom et al., 2018)

NMJ bouton | third instar larval stage, with Scer\GAL4^Toll-6-D42

(Jantrapirom et al., 2018)

synapse, with Scer\GAL4^elav.PU

(Jantrapirom et al., 2020)

Detailed Description

Statement

Reference

The expression of Ubqn^KK100857 under the control of Scer\GAL4^GMR.PS results in rough eye morphology in adult flies. The expression under the control of Scer\GAL4^elav.PLu leads to locomotive dysfunction in both L3 larvae (crawling speed and distance) and in adult flies (climbing score).

(Jantrapirom et al., 2018)

The expression of Ubqn^KK100857 under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^Toll-6-D42 leads to significantly impaired locomotion in adults (in climbing assays) and in third instar larvae (in crawling assays), as compared to controls. Expression under the control of Scer\GAL4^elav.PLu results in adults displaying significant eye degeneration, a decreased preference for the odorant n-amyl acetate and a significant decrease in olfactory learning, as compared to controls. Expression under the control of Scer\GAL4^elav.PLu, but not Scer\GAL4^Toll-6-D42, leads to a small decrease in lifespan, as compared to controls.

Third instar larval expressing Ubqn^KK100857 under the control of Scer\GAL4^elav.PLu present brain morphology defects, ranging from mild (60% of individuals show an asymmetrical distribution of MBs with a midline crossing) to severe (36% of individuals lack some distinctive mushroom body components, such as cx and peduncle); these larvae also present defective neuromuscular junctions, which exhibit a significant decrease in the length of the main branch, but not in the number of branches, and show a significant decrease in the number of terminal boutons, and a reciprocal increase in their size, as compared to controls. Third instar larval expressing Ubqn^KK100857 under the control of Scer\GAL4^Toll-6-D42 present defective neuromuscular junctions, which exhibit a significant decrease in the number of branches, but not in the length of the main branch, and show a significant decrease in the number of boutons, but not in their size, as compared to controls.

(Jantrapirom et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, Ubqn^KK100857 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Scer\GAL4^elav.PLu/TER94^UAS.cHa

(Jantrapirom et al., 2018)

Scer\GAL4^elav.PLu, Ubqn^KK100857 has abnormal locomotor behavior | adult stage phenotype, suppressible by Scer\GAL4^elav.PLu/TER94^UAS.cHa