FlyBase Allele Report: Dmel\p115[KK100066]

General Information

Symbol

Dmel\p115^KK100066

Species

D. melanogaster

Name

FlyBase ID

FBal0230563

Feature type

allele

Associated gene

Dmel\p115

Associated Insertion(s)

Carried in Construct

P{KK100066}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100066}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100066

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

increased cell death | third instar larval stage, with Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

(Ibar and Glavic, 2017)

visible | adult stage, with Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Phenotype Manifest In

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

wing posterior compartment | adult stage, with Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Detailed Description

Statement

Reference

Expression of p115^KK100066 under the control of Scer\GAL4^tub.PU results in lethality before pupation.

Expression of p115^KK100066 under the control of Scer\GAL4^en-e16E leads to a reduction in the posterior compartment size of adult wings, but no obvious disturbance in venation or wing patterning, as compared to controls.

Expression of p115^KK100066 under the control of Scer\GAL4^hh.PU in the posterior compartment of third instar wing discs results in a significant increase in the proportion of cells in S phase and in mitosis in the posterior compartment, as compared to the anterior compartment, and a significant increase in cell death in the posterior compartment, but no significant difference in cell size, as compared to controls.

(Ibar and Glavic, 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, enhanceable by Df(1)ct4b1/+

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, enhanceable by Df(1)ct4b1/+

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, enhanceable by Grasp65^HMS01093, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, enhanceable by Grasp65^HMS01093, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^hh.PU, p115^KK100066 has increased occurrence of cell division | third instar larval stage phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

Scer\GAL4^hh.PU, p115^KK100066 has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, suppressible | partially by Myt1^KK102559, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, suppressible | partially by Myt1^KK102559, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, p115^KK100066 has visible | adult stage phenotype, non-suppressible by Scer\GAL4^en-e16E/stg^UAS.cNa

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has abnormal size | adult stage phenotype, non-suppressible by Scer\GAL4^en-e16E/stg^UAS.cNa

(Ibar and Glavic, 2017)

Scer\GAL4^hh.PU, p115^KK100066 has increased cell death | third instar larval stage phenotype, non-suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

Scer\GAL4^hh.PU, p115^KK100066 has increased occurrence of cell division | third instar larval stage phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Ibar and Glavic, 2017)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, p115^KK100066 has abnormal mitotic cell cycle | third instar larval stage phenotype

(Ibar and Glavic, 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, p115^KK100066 has increased cell size | third instar larval stage phenotype

(Ibar and Glavic, 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, enhanceable by Df(1)ct4b1/+

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, enhanceable by Grasp65^HMS01093, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, suppressible | partially by Myt1^KK102559, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, suppressible by Cdk1^UAS.Tag:MYC, Scer\GAL4^en-e16E

(Ibar and Glavic, 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, p115^KK100066 has wing posterior compartment | adult stage phenotype, non-suppressible by Scer\GAL4^en-e16E/stg^UAS.cNa

(Ibar and Glavic, 2017)

Other

Statement

Reference

Cdk1^UAS.Tag:MYC, Scer\GAL4^hh.PU, p115^KK100066 has wing disc posterior compartment | third instar larval stage phenotype

(Ibar and Glavic, 2017)

BacA\p35^UAS.cHa, Scer\GAL4^hh.PU, p115^KK100066 has wing disc posterior compartment | third instar larval stage phenotype

(Ibar and Glavic, 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The wing posterior compartment size reduction seen in flies expressing p115^KK100066 under the control of Scer\GAL4^en-e16E is enhanced in a Df(1)ct4b1/+ background, or when Dcr-2^Scer\UAS.cDa or Grasp65^HMS01093 is co-expressed; but is suppressed when Cdk1^{Scer\UAS.T:Hsap\MYC} is co-expressed, and partially suppressed when Myt1^KK102559 is co-expressed.

Third instar wing discs expressing both p115^KK100066 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^nub-AC-62 do not show any overt changes in the structure of mitotic spindles, as compared to controls.

Co-expression of stg^Scer\UAS.cNa fails to suppress the wing posterior compartment size reduction seen in flies expressing p115^KK100066 under the control of Scer\GAL4^en-e16E.

Co-expression of Cdk1^{Scer\UAS.T:Hsap\MYC} suppresses the increased proportion of cells in S phase or mitosis in the posterior compartment of third instar wing discs expressing p115^KK100066 under the control of Scer\GAL4^hh.PU, but fails to suppress the increase cell death in these wing discs; no significant changes in cell size or DNA content are seen in these specimens as compared to controls.

(Ibar and Glavic, 2017)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa leads to enhancement of the wing posterior compartment size reduction phenotype seen in adult wings of flies expressing p115^KK100066 under the control of Scer\GAL4^en-e16E.

Co-expression of BacA\p35^Scer\UAS.cHa suppresses the increase cell death, but does not suppress the increased numbers of cells in S phase and mitosis seen in third instar wing discs expressing p115^KK100066 under the control of Scer\GAL4^hh.PU, and additionally leads to an average increase in cell size and an overall increase in the DNA content in the cells in the posterior wing disc, as compared to controls.

(Ibar and Glavic, 2017)

Complementation and Rescue Data

Rescued by

p115^KK100066 is rescued by Scer\GAL4^en-e16E/p115^UAS.Tag:HA

(Ibar and Glavic, 2017)

Comments

The wing posterior compartment size reduction seen in flies expressing p115^KK100066 under the control of Scer\GAL4^en-e16E is rescued when p115^{Scer\UAS.T:Ivir\HA1} is co-expressed.

(Ibar and Glavic, 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103350

P{KK100066}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

p115^KK100066

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close