FlyBase Allele Report: Dmel\PsGEF[Δ21]

General Information

Symbol

Dmel\PsGEF^Δ21

Species

D. melanogaster

Name

FlyBase ID

FBal0221617

Feature type

allele

Associated gene

Dmel\PsGEF

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Higuchi et al., 2009)

Progenitor genotype

P{GawB}NP5114

(Higuchi et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 2.7kb deletion which removes PsGEF exons 1 and 2.

(Higuchi et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Higuchi et al., 2009)

abnormal neuroanatomy | somatic clone

(Higuchi et al., 2009)

fertile

(Higuchi et al., 2009)

viable

(Higuchi et al., 2009)

Phenotype Manifest In

adult mushroom body alpha-lobe

(Higuchi et al., 2009)

adult mushroom body alpha-lobe | somatic clone

(Higuchi et al., 2009)

Detailed Description

Statement

Reference

Egg chambers in PsGEF^Δ21/PsGEF^Δ55 females are morphologically normal and there are no defects in border cell migration.

(Aranjuez et al., 2012)

The alpha lobes of the mushroom bodies are thinner in PsGEF^Δ21 mutants than in wild type. Visualisation of late born alpha/beta lobe neurons indicates that the alpha lobes are thinner than normal in the mutants because the alpha lobes are often short, and their positions along the beta lobes vary.

34% of late-born PsGEF^Δ21 alpha/beta neuron single cell clones have branching defects, with the axon that normally grows towards the alpha lobe being missing.

The morphology of the alpha'/beta' lobes of the mushroom bodies is normal in mutant flies.

(Higuchi et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PsGEF^Δ21 has abnormal neuroanatomy phenotype, enhanceable by Rac1^J11/Rac1[+]

(Higuchi et al., 2009)

NOT Enhanced by

Statement

Reference

PsGEF^Δ21 has abnormal neuroanatomy phenotype, non-enhanceable by Cdc42[+]/Cdc42⁴

(Higuchi et al., 2009)

PsGEF^Δ21 has abnormal neuroanatomy phenotype, non-enhanceable by Rho1^E3.10/Rho1[+]

(Higuchi et al., 2009)

PsGEF^Δ21 has abnormal neuroanatomy phenotype, non-enhanceable by Rac2^Δ/Rac2[+]

(Higuchi et al., 2009)

PsGEF^Δ21 has abnormal neuroanatomy phenotype, non-enhanceable by Mtl[+]/Mtl^Δ

(Higuchi et al., 2009)

PsGEF^Δ21 has abnormal neuroanatomy phenotype, non-enhanceable by Pak[+]/Pak⁶

(Higuchi et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

PsGEF^Δ21 has mushroom body phenotype, enhanceable by Rac1^J11/Rac1[+]

(Higuchi et al., 2009)

NOT Enhanced by

Statement

Reference

PsGEF^Δ21 has adult mushroom body alpha-lobe phenotype, non-enhanceable by Cdc42[+]/Cdc42⁴

(Higuchi et al., 2009)

PsGEF^Δ21 has adult mushroom body alpha-lobe phenotype, non-enhanceable by Rho1^E3.10/Rho1[+]

(Higuchi et al., 2009)

PsGEF^Δ21 has adult mushroom body alpha-lobe phenotype, non-enhanceable by Rac2^Δ/Rac2[+]

(Higuchi et al., 2009)

PsGEF^Δ21 has adult mushroom body alpha-lobe phenotype, non-enhanceable by Mtl[+]/Mtl^Δ

(Higuchi et al., 2009)

PsGEF^Δ21 has adult mushroom body alpha-lobe phenotype, non-enhanceable by Pak[+]/Pak⁶

(Higuchi et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for one of Cdc42⁴, Rho1^E3.10, Rac2^Δ, Pak⁶ or Mtl^Δ has no effect on the phenotype in the alpha lobes of the mushroom bodies that is seen in PsGEF^Δ21 animals.

Heterozygosity for Rac1^J11 enhances the phenotype in the alpha lobes of the mushroom bodies that is seen in PsGEF^Δ21 animals; a pair of alpha/beta lobes is missing in 90% of the double mutants.

(Higuchi et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36343

y¹ PsGEF^Δ21 w^* P{neoFRT}19A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PsGEF^Δ21

(Aranjuez et al., 2012)

dmPsGEF^Δ21

(Higuchi et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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