FlyBase Allele Report: Scer\GAL4[Hand.PA]

General Information

Symbol

Scer\GAL4^Hand.PA

Species

S. cerevisiae

Name

Hand promoter construct of Albrecht

FlyBase ID

FBal0221346

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Hand-GAL4.A}

Also Known As

hand-Gal4, HandC-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Albrecht et al., 2006)

Progenitor genotype

Carried in construct

P{Hand-GAL4.A}

Cytology

Description

Hand regulatory sequences control expression of a GAL4 driver.

(Albrecht et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

Hand

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 17

embryonic cardioblast

(Albrecht et al., 2006)

embryonic pericardial cell

(Albrecht et al., 2006)

embryonic stage 13 -- 14

embryonic dorsal vessel

(Amodio et al., 2012)

embryonic stage 15 -- 16

embryonic/larval dorsal vessel

(Amodio et al., 2012)

larval stage

embryonic/larval heart

(Sénatore et al., 2010)

adult stage

adult pericardial cell

(Hartley et al., 2016, Catterson et al., 2013)

cardial cell

(Lim et al., 2014, Catterson et al., 2013)

enterocyte

(Catterson et al., 2013)

Additional Information

Statement

Reference

In adults, ScerGAL4^Hand.PA drives expression in cardiomyocytes (cardial cells), as well as in pericardial nephrocytes and enterocytes of the gut.

(Catterson et al., 2013)

ScerGAL4^Hand.PA drives expression in all heart cells (cardioblasts and pericardial cells) from embryonic stage 12.

(Albrecht et al., 2006)

Marker for

Reflects expression of

Hand

(Hartley et al., 2016, Lim et al., 2014, Catterson et al., 2013, Albrecht et al., 2006)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Cat^GD1291

(Lim et al., 2014)

abnormal oxidative stress response, with Cat^UAS.cAa

(Lim et al., 2014)

viable | female, with Tmem43^S333L.UAS

(Klinke et al., 2022)

viable | female, with Tmem43^WT.UAS

(Klinke et al., 2022)

Phenotype Manifest In

adult heart, with Klf15^JF02420

(Hartley et al., 2016)

adult heart, with l(2)efl^GD11341

(Wójtowicz et al., 2015)

adult heart, with l(2)efl^{R120G.UASp.Venus}

(Wójtowicz et al., 2015)

adult heart, with l(2)efl^UASp.Venus

(Wójtowicz et al., 2015)

adult heart | male limited, with Tmem43^S333L.UAS

(Klinke et al., 2022)

adult pericardial cell, with Klf15^JF02420

(Ivy et al., 2015)

adult pericardial cell, with Klf15^KK108739

(Ivy et al., 2015)

cardial cell | adult stage, with mys^GD15002

(Catterson et al., 2013)

embryonic/larval heart | third instar larval stage, with loh^GD5502

(Drechsler et al., 2013)

embryonic/larval heart | third instar larval stage, with loh^NIG.6232R

(Drechsler et al., 2013)

embryonic/larval heart | third instar larval stage, with prc^GD6085

(Drechsler et al., 2013)

embryonic/larval heart | third instar larval stage, with prc^KK106057

(Drechsler et al., 2013)

embryonic/larval pericardial cell | third instar larval stage, with loh^GD5502

(Drechsler et al., 2013)

embryonic/larval pericardial cell | third instar larval stage, with loh^NIG.6232R

(Drechsler et al., 2013)

embryonic/larval pericardial cell | third instar larval stage, with prc^GD6085

(Drechsler et al., 2013)

embryonic/larval pericardial cell | third instar larval stage, with prc^KK106057

(Drechsler et al., 2013)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sod2^UAS.cMa/Scer\GAL4^Hand.PA is an enhancer of abnormal oxidative stress response phenotype of Scer\GAL4^Ugt36A1.PK, Sod1^UAS.cWa

(Lim et al., 2014)

Scer\GAL4^Hand.PA/Sod1^UAS.cWa is an enhancer of abnormal oxidative stress response phenotype of Scer\GAL4^Ugt36A1.PK, Sod2^UAS.cMa

(Lim et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Fit1^GD17050, Scer\GAL4^Hand.PA has cardial cell | adult stage phenotype, enhanceable by Fit2^GD1300, Scer\GAL4^Hand.PA

(Catterson et al., 2013)

Enhancer of

Statement

Reference

Fit2^GD1300, Scer\GAL4^Hand.PA is an enhancer of cardial cell | adult stage phenotype of Fit1^GD17050, Scer\GAL4^Hand.PA

(Catterson et al., 2013)

NOT Enhancer of

Statement

Reference

Sod2^UAS.cMa/Scer\GAL4^Hand.PA is a non-enhancer of adult heart phenotype of Scer\GAL4^Ugt36A1.PK, Sod1^UAS.cWa

(Lim et al., 2014)

Scer\GAL4^Hand.PA/Sod1^UAS.cWa is a non-enhancer of adult heart phenotype of Scer\GAL4^Ugt36A1.PK, Sod2^UAS.cMa

(Lim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^Hand.PA/Creld^UAS.Tag:HA fails to rescue Creld^Δ51

(Paradis et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Hand.PA

Name Synonyms

Hand promoter construct of Albrecht

Secondary FlyBase IDs

References (18)

Report Sections

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