FlyBase Allele Report: Dmel\Cdk8[K185]

Imprecise excision of P{EP}I-2^GE26681, resulting in retention of the P{EP} extremity in I-2 and deletion of 882 bp proximal to the original P{EP}I-2^GE26681 insertion, including the C-terminal one third of Cdk8 protein-coding sequences.

(Loncle et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:9,836,757..9,837,638 [-]

Comment:

Approximate endpoints of an 882 bp deletion associated with the imprecise excision of P{EP}I-2^GE26681.

(Loncle et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of developmental and epileptic encephalopathy 87

CEA

(Chung et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of developmental and epileptic encephalopathy 87

is ameliorated by Hsap\CDK19^UAS.Tag:HA

(Chung et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior pupal spiracle

(Xie et al., 2015)

leg | somatic clone - Minute background

(Loncle et al., 2007)

mesothoracic bristle | somatic clone

(Loncle et al., 2007)

mesothoracic bristle | somatic clone - Minute background

(Loncle et al., 2007)

prothoracic tarsal segment | somatic clone

(Loncle et al., 2007)

sex comb tooth | somatic clone

(Loncle et al., 2007)

Detailed Description

Statement

Reference

Pupariation is delayed by approximately 2-3 days in homozygous animals. This developmental delay is slightly rescued by feeding with 20-hydroxyecdysone.

96% of homozygous prepupae fail to evert their anterior spiracles, and the prepupae are partially separated from the pupal case.

The level of ecdysteroids is significantly lower than normal in homozygous wandering third instar larvae and white prepupae.

(Xie et al., 2015)

Homozygous larvae have a significantly increased triglyceride content compared to controls.

(Zhao et al., 2012)

Generation of Cdk8^K185 clones specifically in the leg (using Scer\GAL4^Dll-em212 and Scer\FLP1^Scer\UAS.cUa) and in a Minute background results in severely stunted legs. In an other wild type background, these clones result in tarsal segmentation defects in all three paris of adult legs. Male prothoracic (T1) legs exhibit sex combs with slightly elevated teeth number and discontinuities in the tooth alignment, leading to irregularly grouped, fragmented sex combs. Leg size is relatively unaffected, but the most proximal tarsal joint is usually abnormal.

Generation of large Cdk8^K185 clones specifically in the eye (using Scer\FLP1^ey.PB) in a Minute background results in full-sized eyes with mostly normal differentiated ommatidia.

Heat-induced Cdk8^K185 clones in the adult notum are associated with loss of bristles.

Generation of large Cdk8^K185 clones specifically in the eye (using Scer\GAL4^ap-md544 and Scer\FLP1^Scer\UAS.cUa) in a Minute background results in extensive loss of notal macrochaetes.

(Loncle et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(3L)AC1/Cdk8^K185 has increased mortality during development phenotype, suppressible by Hsap\CDK19^UAS.Tag:HA/Scer\GAL4^Act5C.PI

(Chung et al., 2020)

NOT suppressed by

Statement

Reference

Df(3L)AC1/Cdk8^K185 has lethal phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Hsap\CDK19^{T196A.UAS.Tag:HA}

(Chung et al., 2020)

Df(3L)AC1/Cdk8^K185 has lethal phenotype, non-suppressible by Hsap\CDK19^{Y32H.UAS.Tag:HA}/Scer\GAL4^Act5C.PI

(Chung et al., 2020)

Phenotype Manifest In

Other

Statement

Reference

Cdk8^K185, kto^T241 has sex comb tooth | somatic clone phenotype

(Loncle et al., 2007)

Cdk8^K185, kto^T241 has prothoracic tarsal segment | somatic clone phenotype

(Loncle et al., 2007)

Cdk8^K185, kto^T241 has eye | somatic clone - Minute background phenotype

(Loncle et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The phenotypes of Cdk8^K185 CycC^Y5 double mutants are similar to those of the single mutants.

(Xie et al., 2015)

The distal leg phenotypes provoked by Cdk8^K185, kto^T241 double mutant clones induced specifically in the leg (using Scer\GAL4^Dll-em212 and Scer\FLP1^Scer\UAS.cUa) are indistinguishable from kto^T241 alone but distinct from Cdk8^K185 alone, both for sex comb differentiation and for overall leg structure.

The phenotypes of Cdk8^K185, kto^T241 double mutant clones induced specifically in the eye (using Scer\FLP1^ey.PB) in a Minute background are indiscernible from kto^T241 alone.

(Loncle et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3L)AC1/Cdk8^K185 is rescued by Scer\GAL4^Act5C.PI/Cdk8^UAS.cUa

(Chung et al., 2020)

Cdk8^K185 is rescued by Cdk8^EGFP

(Xu et al., 2020)

Cdk8^K185 is rescued by Cdk8^EGFP

(Xie et al., 2015)

Cdk8^K185 is rescued by Cdk8^Ubi-p63E.PL

(Loncle et al., 2007)

Comments

Cdk8^{T:Avic\GFP-EGFP} rescues the developmental delay to pupariation, the failure of prepupal anterior spiracle eversion and the prepupal lethality of Cdk8^K185 animals.

(Xie et al., 2015)

Cdk8^K185 homozygotes are fully rescued to yield viable, morphologically normal, fertile adults by expression of Cdk8^Ubi-p63E.PL.

(Loncle et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CDK8^K185

(Tang et al., 2018)

Cdk8^K185

(Chung et al., 2020, Zhao et al., 2012, de Ayala Alonso et al., 2007, Loncle et al., 2007)

cdk8^K185

(Kuang et al., 2020, Li et al., 2020, Xu et al., 2020, Xie et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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